MicroRNAs (miRNAs) are small, subtypes of RNA that are 22-25 nucleotides in length that regulate the gene expression by targeting homologous sequences in messenger RNA (mRNA). Their unusual expressions have been observed in various types of cancers. In this paper we first briefly outline of multiple pairwise alignment of primiRNA- 125 of three organisms and secondary structure. We then discuss in detail on binding sites of miRNA-125 with different target genes to known the binding energy using computational tool. Finally, we discuss on mechanism of miRNA-125 over-expression causes different types of leukemia due to abnormal expression of gene present on chromosome 21. Thus we have reported that one of the major causes of leukemia is abnormal expression of miRNA- 125.
Ful
