Caracterización de un modelo de sistema de información interorganizacional para el sector de la edificación domótica

El sector de la edificación es uno de los principales sectores económicos con grandes repercusiones en el conjunto de la sociedad y en los valores culturales que entraña el patrimonio arquitectónico y, sin embargo, carece de una regulación acorde. La imparable evolución de las TIC y la cultura de la competencia han provocado la aparición de nuevos servicios y operadores. Por ello, el presente estudio, basado en un trabajo de investigación empírico, propone la caracterización de un sistema de información interorganizacional (SIIO) entre los diversos agentes que configuran la cadena de valor del sector de la edificación. El objetivo es la mejora de gestión en las interrelaciones entre los diferentes agentes de cara a abordar proyectos de edificación más complejos, contribuyendo, en particular, a la mejora de la calidad de la vivienda y, por tanto, a la calidad de vida de la sociedad en general

Protein O-Fucosyltransferase 1 Undergoes Interdomain Flexibility in Solution

Protein O-fucosyltransferase 1 (PoFUT1) is a GT-B fold enzyme that fucosylates proteins containing EGF-like repeats. GT-B glycosyltransferases have shown a remarkable grade of plasticity adopting closed and open conformations as a way of tuning their catalytic cycle, a feature that has not been observed for PoFUT1. Here, we analyzed Caenorhabditis elegans PoFUT1 (CePoFUT1) conformational behavior in solution by atomic force microscopy (AFM) and single-molecule fluorescence resonance energy transfer (SMF-FRET). Our results show that this enzyme is very flexible and adopts mainly compact conformations and to a lesser extend a highly dynamic population that oscillates between compact and highly extended conformations. Overall, our experiments illustrate the inherent complexity of CePoFUT1 dynamics, which might play a role during its catalytic cycle.ARAID: MEC (CTQ2013-44367-C2-2-P, BFU2016-75633-P and PID2019-105451GBI00 to RH-G, CTQ2017-85658-R and CTQ2014-56370-R to AO)Gobierno de Aragón (E35_R20 and LMP58_18)FEDER (2014-2020) funds for ‘Building Europe from Aragón’Juan de la Cierva fellowship IJCI-2017-3287

A Quantum Dot-Based FLIM Glucose Nanosensor

In the last few years, quantum dot (QD) nanoparticles have been employed for bioimaging and sensing due to their excellent optical features. Most studies have used photoluminescence (PL) intensity-based techniques, which have some drawbacks, especially when working with nanoparticles in intracellular media, such as fluctuations in the excitation power, fluorophore concentration dependence, or interference from cell autofluorescence. Some of those limitations can be overcome with the use of time-resolved spectroscopy and fluorescence lifetime imaging microscopy (FLIM) techniques. In this work, CdSe/ZnS QDs with long decay times were modified with aminophenylboronic acid (APBA) to achieve QD-APBA conjugates, which can act as glucose nanosensors. The attachment of the boronic acid moiety on the surface of the nanoparticle quenched the PL average lifetime of the QDs. When glucose bonded to the boronic acid, the PL was recovered and its lifetime was enhanced. The nanosensors were satisfactorily applied to the detection of glucose into MDA-MB-231 cells with FLIM. The long PL lifetimes of the QD nanoparticles made them easily discernible from cell autofluorescence, thereby improving selectivity in their sensing applications. Since the intracellular levels of glucose are related to the metabolic status of cancer cells, the proposed nanosensors could potentially be used in cancer diagnosis.This research was funded by grants CTQ2014-56370-R and CTQ2017-85658-R from the Spanish Ministerio de Ciencia, Innovación y Universidades, Agencia Estatal de Investigación (AEI), and the European Regional Development Fund (ERDF)

Circularly Polarized Luminescence of [6]Helicenes through Excited- State Intramolecular Proton Transfer

This project received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (Grant Agreements No. 716139 and No. 677023), the Swiss National Science Foundation (SNSF, PP00P2_170534, PP00P2_198900), Project PGC2018-101181-B-I00 funded by MCIN/AEI/ 10.13039/501100011033 FEDER "Una manera de hacer Europa", grant A-FQM-230-UGR20 funded by FEDER/Junta de Andalucia-Consejeria de Transformacion Economica, Industria, Conocimiento y Universidades and the German Academic Exchange Service (DAAD, postdoc fellowship to D. G.). Open access funding provided by the University of Zurich.We present the concept of combining circularly polarized luminescence (CPL) and excited-state intramolecular proton transfer (ESIPT) features into a single molecule as a strategy to generate high-performance ESIPT-based CPL materials. For this purpose, a [6]helicene bearing two ESIPT structural units was synthesized using a double Suzuki-Miyaura reaction and a double C(sp(2))-H hydroxylation approach. The photophysical properties of the doubly hydroxylated [6]helicene were studied in parallel with a non-hydroxylated [6]helicene control compound, revealing that the presence of a chiral [6]helicene unit results in a strong CPL response and the presence of the ESIPT units in a considerable red shift. The red-shifted emission along with the outstanding g(lum) (approximate to 10(-2)) and a large Stokes shift makes the doubly hydroxylated [6]helicene a promising candidate for use in optoelectronics.European Research Council (ERC) 716139 677023Swiss National Science Foundation (SNSF) PP00P2_170534 PP00P2_198900Deutscher Akademischer Austausch Dienst (DAAD)University of ZurichMCIN/AEI FEDER "Una manera de hacer Europa" PGC2018-101181-B-I00FEDER/Junta de Andalucia-Consejeria de Transformacion Economica, Industria, Conocimiento y Universidades A-FQM-230-UGR2

Mitochondrial pH Nanosensors for Metabolic Profiling of Breast Cancer Cell Lines

We thank the company Nanogetic S.L. (Granada, Spain) for support with the synthesis of the SS peptides and the Centro de Instrumentacion Cientifica (CIC) of the Universidad de Granada for use of the TEM facilities.The main role of mitochondria, as pivotal organelles for cellular metabolism, is the production of energy (ATP) through an oxidative phosphorylation system. During this process, the electron transport chain creates a proton gradient that drives the synthesis of ATP. One of the main features of tumoral cells is their altered metabolism, providing alternative routes to enhance proliferation and survival. Hence, it is of utmost importance to understand the relationship between mitochondrial pH, tumoral metabolism, and cancer. In this manuscript, we develop a highly specific nanosensor to accurately measure the intramitochondrial pH using fluorescence lifetime imaging microscopy (FLIM). Importantly, we have applied this nanosensor to establish differences that may be hallmarks of different metabolic pathways in breast cancer cell models, leading to the characterization of different metabophenotypes.Spanish Ministerio de Ciencia, Innovacion y Universidades CTQ2014-56370-R CTQ2017-86568-REuropean Union (EU)Spanish Agencia Estatal de InvestigacionFundacion Ramon Arece

Implementación de laboratorios 2.0 para mecánica de fluidos

Se ha desarrollado una serie de mejoras en aras de la digitalización de los laboratorios de Mecánica de Fluidos e Hidráulica que se cursan en las asignaturas de fluidos de 2º curso de diversos grados en la ETSIME de la Universidad Politécnica de Madrid. Esta experiencia se realiza durante el curso 2020-21, acelerado en parte debido a los problemas por la pandemia en el curso 2019-20. Se han creado unos videos resumen de las prácticas, actualizado los guiones y mantenido las sesiones presenciales en grupos reducidos. Para la entrega de resultados se han desarrollado unas aplicaciones en Matlab que el alumno debe emplear. En estas aplicaciones el alumno realiza o elige los cálculos o soluciones a lo visto en el laboratorio, realiza minijuegos y discute los resultados obtenidos. La mejora global en la entrega de resultados frente al método clásico en papel se sitúa en torno al 11%, siendo más importante en hombres que en mujeres. La apreciación de los alumnos, en base a encuestas, de la nueva metodología es bastante buena con valores superiores al 4 sobre 5 en todos los grupos en ambos géneros

Expression of Gls and Gls2 glutaminase isoforms in astrocytes

The expression of glutaminase in glial cells has been a controversial issue and matter of debate for many years. Actually, glutaminase is essentially considered as a neuronal marker in brain. Astrocytes are endowed with efficient and high capacity transport systems to recapture synaptic glutamate which seems to be consistent with the absence of glutaminase in these glial cells. In this work, a comprehensive study was devised to elucidate expression of glutaminase in neuroglia and, more concretely, in astrocytes. Immunocytochemistry in rat and human brain tissues employing isoform-specific antibodies revealed expression of both Gls and Gls2 glutaminase isozymes in glutamatergic and GABAergic neuronal populations as well as in astrocytes. Nevertheless, there was a different subcellular distribution: Gls isoform was always present in mitochondria while Gls2 appeared in two different locations, mitochondria and nucleus. Confocal microscopy and double immunofluorescence labeling in cultured astrocytes confirmed the same pattern previously seen in brain tissue samples. Astrocytic glutaminase expression was also assessed at the mRNA level, real-time quantitative RT-PCR detected transcripts of four glutaminase isozymes but with marked differences on their absolute copy number: the predominance of Gls isoforms over Gls2 transcripts was remarkable (ratio of 144:1). Finally, we proved that astrocytic glutaminase proteins possess enzymatic activity by in situ activity staining: concrete populations of astrocytes were labeled in the cortex, cerebellum and hippocampus of rat brain demonstrating functional catalytic activity. These results are relevant for the stoichiometry of the Glu/Gln cycle at the tripartite synapse and suggest novel functions for these classical metabolic enzymes

Application of new information and communication methodologies (ICT) for laboratory teaching in applied physics and physical chemistry

En este artículo se presenta un trabajo de innovación en metodología docente que tiene como objetivo la mejora en el aprendizaje de las enseñanzas prácticas de laboratorio de la asignatura de Física Aplicada y Fisicoquímica de la Licenciatura de Farmacia de la Universidad de Granada. La enseñanza práctica de diversas asignaturas de esta facultad tradicionalmente se ha realizado con la colaboración de los “monitores de prácticas”, los cuales se encargan de ayudar a sus compañeros en la realización de las prácticas de laboratorio, estando siempre coordinados y supervisados por un profesor. Puesto que este sistema tiene sus carencias y limitaciones, con la metodología presentada en este trabajo se pretende que desaparezca la figura del monitor de prácticas persiguiendo al mismo tiempo que el alumno sea más receptivo y muestre mayor interés. La experiencia nos demuestra que resulta fundamental la visualización de la manipulación y las operaciones necesarias para la realización de una práctica. Las nuevas tecnologías audiovisuales de la información y la comunicación (TIC) ponen al servicio de la enseñanza herramientas muy didácticas que hacen más atractivo el aprendizaje. Con tal fin, se ha elaborado para la asignatura antes mencionada un vídeo de cada práctica en formato DVD, en los que, con objeto de guiar al alumno en el trabajo experimental, se muestra la manipulación del material y reactivos empleados en cada práctica, uso de la instrumentación específica, buenas prácticas en un laboratorio y adquisición y análisis de datos. El objetivo que se persigue es que el alumno realice la práctica simultáneamente al desarrollo del vídeo, con la opción de interactuar con el vídeo y con el propio profesor presente en la sesión. En definitiva, mediante esta metodología de prácticas innovadora se pretende la captación de la atención del alumno, su motivación, promoción del trabajo autónomo y capacidad de iniciativa facilitando de esta forma su capacidad de autoaprendizaje. Todo lo comentado, contribuye a potenciar la adaptación de los actuales estudios universitarios al Espacio Europeo de Educación Superior.The aim of this innovative teaching work is the improvement in the learning process of laboratory practical sessions of the subject ‘Applied Physics and Physical Chemistry’ of the Pharmacy degree syllabuses at the University of Granada. The laboratory practical sessions of several subjects in this Faculty have been traditionally carried out with the collaboration of ‘laboratory demonstrators’, who help to their classmates in the performance of the experiments, being always coordinated and project, we expect on one hand the figure of ‘laboratory demonstrators’ to disappear, and on the other hand to achieve that the student is more receptive and shows a higher interest in the practical sessions. Our experience proves that it is very important for the student to visualize the manipulation and methodology needed for the performance of the experiments in the laboratory. The new audiovisual Information and Communication Technologies (ICT) provide very didactic tools, which make the learning more attractive. With this purpose, we have prepared a DVD movie of each experiment planned in the practical sessions of the subject. These movies show the manipulation of the material and reagents, the use of specific instrumentation, good behaviour in the laboratory, and data acquisition and analysis, with the aim of guiding the student in the experimental work. The student will perform the experiments simultaneously to the movie projection, with the possibility of interacting with the movie itself and the senior professor present during the session. In short, we expect to enhance the motivation and own-initiative of the students, facilitating their self-learning, by means of an innovative teaching methodology that contributes to promote the adaptation to the European Space for Higher Education.El proyecto ha sido financiado por la Unidad de Innovación Docente de la Universidad de Granada (Proyecto de Innovación Docente, Código Nº 08-109