422 research outputs found
Basic Concepts in Conservation Education
Get PDFWhenever wildlife conservation is discussed there is usually almost unanimous agreement that education offers the best if not the only solution to many of the conservation problems still only partly solved. This in spite of the fact that during the past fifteen years our colleges and universities have been turning out scores oÂŁ wildlife technicians each year. During this same period the grade and high schools have included more and more conservation teaching in their curricula, and every other educational medium has been used to some extent to present the principles and preach the practices of conservation
The nonlinear anomalous lattice elasticity associated with the high-pressure phase transition in spodumene: A high precission static compression study
Full text linkThe high-pressure behavior of the lattice elasticity of spodumene, LiAlSi2O6,
was studied by static compression in a diamond-anvil cell up to 9.3 GPa.
Investigations by means of single-crystal XRD and Raman spectroscopy within the
hydrostatic limits of the pressure medium focus on the pressure ranges around
similar to 3.2 and similar to 7.7 GPa, which have been reported previously to
comprise two independent structural phase transitions. While our measurements
confirm the well-established first-order C2/c-P2(1)/c transformation at 3.19
GPa (with 1.2% volume discontinuity and a hysteresis between 0.02 and 0.06
GPa), both unit-cell dimensions and the spectral changes observed in
high-pressure Raman spectra give no evidence for structural changes related to
a second phase transition. Monoclinic lattice parameters and unit-cell volumes
at in total 59 different pressure points have been used to re-calculate the
lattice-related properties of spontaneous strain, volume strain, and the bulk
moduli as a function of pressure across the transition. A modified Landau free
energy expansion in terms of a one component order parameter has been developed
and tested against these experimentally determined data. The Landau solution
provides a much better reproduction of the observed anomalies than any
equation-of-state fit to data sets truncated below and above P (tr), thus
giving Landau parameters of K (0) = 138.3(2) GPa, K' = 7.46(5), lambda (V) =
33.6(2) GPa, a = 0.486(3), b = -29.4(6) GPa and c = 551(11) GPa
Intentions to Smoke Cigarettes Among Never-Smoking US Middle and High School Electronic Cigarette Users: National Youth Tobacco Survey, 2011–2013
Get PDFIntroduction: Electronic cigarette (e-cigarette) use is increasing rapidly, and the impact on youth is unknown. We assessed associations between e-cigarette use and smoking intentions among US youth who had never smoked conventional cigarettes. Methods: We analyzed data from the nationally representative 2011, 2012, and 2013 National Youth Tobacco Surveys of students in grades 6–12. Youth reporting they would definitely not smoke in the next year or if offered a cigarette by a friend were defined as not having an intention to smoke; all others were classified as having positive intention to smoke conventional cigarettes. Demographics, pro-tobacco advertisement exposure, ever use of e-cigarettes, and ever use of other combustibles (cigars, hookah, bidis, kreteks, and pipes) and noncombustibles (chewing tobacco, snuff, dip, snus, and dissolvables) were included in multivariate analyses that assessed associations with smoking intentions among never-cigarette-smoking youth. Results: Between 2011 and 2013, the number of never-smoking youth who used e-cigarettes increased 3-fold, from 79,000 to more than 263,000. Intention to smoke conventional cigarettes was 43.9% among ever e-cigarette users and 21.5% among never users. Ever e-cigarette users had higher adjusted odds for having smoking intentions than never users (adjusted odds ratio = 1.70, 95% confidence interval = 1.24–2.32). Those who ever used other combustibles, ever used noncombustibles, or reported pro-tobacco advertisement exposure also had increased odds for smoking intentions. Conclusion: In 2013, more than a quarter million never-smoking youth used e-cigarettes. E-cigarette use is associated with increased intentions to smoke cigarettes, and enhanced prevention efforts for youth are important for all forms of tobacco, including e-cigarettes
A Rapid Flp-In System for Expression of Secreted H5N1 Influenza Hemagglutinin Vaccine Immunogen in Mammalian Cells
Get PDFContinuing transmissions of highly pathogenic H5N1 viruses in poultry and humans underscores the need for a rapid response to potential pandemic in the form of vaccine. Recombinant technologies for production of immunogenic hemagglutinin (HA) could provide an advantage over the traditional inactivated vaccine manufacturing process. Generation of stably transfected mammalian cells secreting properly folded HA proteins is important for scalable controlled manufacturing.We have developed a Flp-In based 293 stable cell lines through targeted site-specific recombination for expression of secreted hemagglutinin (HA) proteins and evaluated their immunogenicity. H5N1 globular domain HA1(1-330) and HA0(1-500) proteins were purified from the supernatants of 293 Flp-In stable cell lines. Both proteins were properly folded as confirmed by binding to H5N1-neutralizing conformation-dependent human monoclonal antibodies. The HA0 (with unmodified cleavage site) was monomeric, while the HA1 contained oligomeric forms. Upon rabbit immunization, both HA proteins elicited neutralizing antibodies against the homologous virus (A/Vietnam/1203/2004, clade 1) as well as cross-neutralizing antibodies against heterologous H5N1 clade 2 strains, including A/Indonesia/5/2005. These results exceeded the human antibody responses against the inactivated sub-virion H5N1 vaccine.Our data suggest that the 293 Flp-In system could serve as a platform for rapid expression of HA immunogens in mammalian cells from emerging influenza strains
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A Search for Dark Higgs Bosons
Get PDFRecent astrophysical and terrestrial experiments have motivated the proposal
of a dark sector with GeV-scale gauge boson force carriers and new Higgs
bosons. We present a search for a dark Higgs boson using 516 fb-1 of data
collected with the BABAR detector. We do not observe a significant signal and
we set 90% confidence level upper limits on the product of the Standard
Model-dark sector mixing angle and the dark sector coupling constant.Comment: 7 pages, 5 postscript figures, published version with improved plots
for b/w printin
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