An overview of composition, properties, and applications of Biodentine

A series of events leads to loss of tooth structure by dental caries, tooth wear and trauma, which is often replaced by inert dental materials that replace the bulk of the tooth. If pulp health is affected, a series of interventions need to be undertaken. Initially, the pulp vitality needs to be maintained. Later, elimination of infection and filling of the pulp space is necessary. When pulpal involvement occurs the choice of material has to change, and materials that interact with the pulp are indicated. Interactive materials used for dental procedures include calcium hydroxide in its various presentations and hydraulic calcium silicate cement.  Biodentine is a promising dentine substitute that has been recently introduced in dentistry. Although many other materials like Glass Ionomer Cement (GIC), composite and Mineral Trioxide Aggregate (MTA) are available for repair of dentin loss in tooth structure, none of them possesses ideal properties. Despite many advantages, MTA has been replaced by Biodentine, which is a new calcium silicate-based material, due to its limitations. It has good handling properties, short setting time, and improved mechanical properties. Biodentine was designed explicitly as a "dentine replacement," with applications ranging from endodontic repair to pulp capping

Disinfection of impression materials: A comprehensive review of disinfection methods

Impression making is one of the most common procedures that are performed by dentists in day-to-day practice. These impressions can act as vehicles of transmission and carry various types of microorganisms, which further cause diseases like Hepatitis B, C, HIV, Tuberculosis etc. This contamination and cross contamination of microorganisms can be prevented by disinfecting the impressions immediately after removing from the mouth and label them as disinfected. Usually the impressions are placed under running water to remove saliva and blood, but this will eliminate the disease-causing microorganisms, so a standard protocol to disinfect the impressions and casts should be known to dentists and dental personnel. Various methods of impression disinfection like chemical disinfection, Microwave, Autoclave, Ultraviolet radiation have been described in literature having their own advantages, disadvantages and effects on impression material and casts. Recently antimicrobials and nanoparticles have been incorporated into the impression material itself to make it self- disinfecting. This will not only disinfect the impression material from inside but also disinfect the impressions from the time it is inserted in patient’s mouth.  A broad search on the literature available was performed to provide knowledge about mechanism of action, concentration of usage along with commercial preparations available of different disinfectants. This review article will enhance the knowledge and improve the behavior of dental health care workers about impression disinfection

Prosthodontic rehabilitation of a mucormycosis patient: a case report

Maxillofacial defects can result from congenital disabilities, cancer surgery, trauma, infection, or disease. Facial deformities can affect how a person looks, feels about themselves, and interacts with others. It can significantly impair phonetics, mastication, and deglutition and cause facial deformation. Maxillectomy due to mucormycosis is one such maxillofacial defect and it becomes essential to rehabilitate these cases with modified techniques based on the extension of intraoral defect, the severity, the degree of resection, the type of mucormycosis, the stability of lesions over time, the presence of contiguous disease, the accessibility of dental and prosthetic resources, and patient expectations. The prosthetic reconstruction with a maxillofacial prosthesis can restore function and appearance, comfort, and quality of life. The prosthesis should be simple to handle, easy to maintain, biocompatible, light in weight, and convenient for future adjustments. The maxillofacial surgeon, oncologist, and reconstructive dentist should work together to develop a treatment plan based on these considerations. This case report provides the current treatment options for these patients and rehabilitation of the defect. It also discusses the issues that need to be addressed during the planning of prosthetic treatment and highlights some challenges the clinicians face in providing prosthetic treatment for mucormycosis patients

Resin-bonded fixed partial denture as a cost-effective prosthesis for missing maxillary lateral incisor in a cleft lip and palate patient: a case report

Literature suggests 66.7% of dental abnormalities among patients with cleft lip and palate (CLP). Besides posing functional difficulties, dental anomalies also raise significant aesthetic concerns among these patients, especially with missing teeth in the anterior region. Among various treatment choices like a removable partial denture, fixed and implant treatments available in the prosthetic rehabilitation of missing teeth in CLP subjects, Maryland bridges offer a conservative and cost-effective alternative in short-span edentulous spaces while carrying the advantages of supragingival marginal preparations and less intensive working times. The availability of advanced self-etch adhesive systems that chemically bond to both the tooth and metal surfaces offer these prostheses more longevity. This case report presents the fabrication of a resin-bonded fixed partial denture in the prosthetic rehabilitation of a missing maxillary lateral incisor in a 23-year-old female patient with CLP who was unwilling for orthodontic care

The effect of Fit-checking material and various subsequent cleaning methods on the wettability of the dentin surface: an in vitro study

Background: GC Fit-checker is a modified polyvinyl siloxane impression material exclusively used to check the internal fit and improve the marginal fit of indirect restorations. An unpolymerized organic film is known to be leftover on the bonding surfaces after the silicone disclosing procedure. Residual silicone film being hydrophobic may alter the wettability of the cement to the tooth/metal surface, thus having a detrimental effect on the bond strength and retention of the restoration.   Aim: This study aimed to evaluate the wetting of luting liquid (GIC) to tooth dentin surface after application of Fit-checker and evaluate the efficiency of various surface treatments in removing the residual silicone film. Materials and Methods: Extracted human molars were mounted on the acrylic block, and the tooth occlusal surface was ground flat till the dentin exposure. All the specimens were assigned into five groups: Group 1: without application of Fit-checker (control group); Group 2: without any surface treatment after peeling off Fit-checker; Group 3: surface treatment with wet pumice; Group 4: 37% phosphoric acid treatment; Group 5: 10% polyacrylic acid treatment. Later, Type 1 Glass Ionomer Cement (GIC) liquid drop was placed on the dentin and photographs were made horizontally using a standardized procedure. Contact angles were measured using AUTOCAD software. Obtained values were statistically analyzed using the One-way ANOVA test and Tukey’s Post hoc test. Samples of each group were examined using the scanning electron microscope. Results: Statistically significant difference was observed among all the groups except between Group 4 and Group 2 (p > 0.05). SEM images of various groups showed a significant difference in roughness patterns. Conclusion: Surface treatment with pumice and the rotary brush was an effective method among the three in cleaning the residual silicone film

Mineral Trioxide Aggregate: an overview of composition, properties and clinical applications

“Mineral trioxide aggregate” is a cementitious material, which is popular by its trade name MTA. It was first introduced in the year 1993 by Mohmoud Torabinejad at Loma Linda University in California, USA. MTA is a powder mixture of Portland cement clinker, bismuth oxide, and gypsum. It has gained a lot of importance in dentistry in recent years. This importance is because of its extensive use as apical restorative material as well as a medicament for Apexogenesis and Apexification treatment. As it sets by hydration process, the byproducts of insoluble calcium silicate hydrate and alkaline calcium hydroxide offer unique stability and potential to enhance hard tissue regeneration.  This article reviewed the composition, types, properties of MTA and also its applications in the practice of dentistry

An overview of advances in glass ionomer cements

Glass-ionomer dental cements (GICs) are aesthetic direct restorative materials with anticariogenic activity. Glass-ionomers are composed of alumino-silicate glass powder and poly acrylic acid liquid. The significant characteristics of GICs among restorative materials are their ability to bond to moist tooth structure without any pre-treatment and to provide a prolonged period of fluoride release, which prevents subsequent tooth decay (caries). These characteristics, along with the materials\u27 acceptable aesthetics and biocompatibility, make them popular and desirable for use in medical and dental applications. However, GICs exhibit poor mechanical qualities and moisture sensitivity. To improve their mechanical and physical qualities, the GIC powders have undergone extensive formulation and modification. This paper provides an overview of various fillers used to enhance the mechanical and physical properties of GICs

Rubisco-bis-phosphate oxygenase (RuBP)- A potential housekeeping gene for qPCR assays in tea

The present experiment is an effort to find a stable reference gene in Camellia sinensis and Camellia assamica under different biotic and abiotic stresses. This study evaluate the variation in gene expression across tea leaf tissues in nine experiments. The suitability of 18S rRNA, 26S rRNA, rubiscobis- phosphatase oxygenase (RuBP) and Camellia tubulin (CaT) as reference genes were validated by geNorm and BestKeeper programs. The finding reveals 18S rRNA and RuBP to be the most stably expressed housekeeping genes, the latter being the first report of its kind in tea. The finding paves the way for their application in accurate quantification of trait specific gene expression and other genomic studies in tea.Keywords: Camellia sinensis, Camellia assamica, qPCR, BestKeeper, geNorm, housekeeping gen

Next-generation sequencing using microfluidic PCR enrichment for molecular autopsy.

BACKGROUND: We aimed to determine the mutation yield and clinical applicability of "molecular autopsy" following sudden arrhythmic death syndrome (SADS) by validating and utilizing low-cost high-throughput technologies: Fluidigm Access Array PCR-enrichment with Illumina HiSeq 2000 next generation sequencing (NGS). METHODS: We validated and optimized the NGS platform with a subset of 46 patients by comparison with Sanger sequencing of coding exons of major arrhythmia risk-genes (KCNQ1, KCNH2, SCN5A, KCNE1, KCNE2, RYR2). A combined large multi-ethnic international SADS cohort was sequenced utilizing the NGS platform to determine overall molecular yield; rare variants identified by NGS were subsequently reconfirmed by Sanger sequencing. RESULTS: The NGS platform demonstrated 100% sensitivity for pathogenic variants as well as 87.20% sensitivity and 99.99% specificity for all substitutions (optimization subset, n = 46). The positive predictive value (PPV) for NGS for rare substitutions was 16.0% (27 confirmed rare variants of 169 positive NGS calls in 151 additional cases). The overall molecular yield in 197 multi-ethnic SADS cases (mean age 22.6 ± 14.4 years, 68% male) was 5.1% (95% confidence interval 2.0-8.1%), representing 10 cases carrying pathogenic or likely pathogenic risk-mutations. CONCLUSIONS: Molecular autopsy with Fluidigm Access Array and Illumina HiSeq NGS utilizing a selected panel of LQTS/BrS and CPVT risk-genes offers moderate diagnostic yield, albeit requiring confirmatory Sanger-sequencing of mutational variants

Mycobacterium tuberculosis ClpP Proteases Are Co-transcribed but Exhibit Different Substrate Specificities

PMCID: PMC3613350This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited