3D-QSAR study of pyrazolo[3,4-d]pyrimidines and 1,3,4-thiadiazoles as BCR-ABL1 inhibitors

The treatment of chronic myeloid leukemia (CML) was revolutionized by introducing Bcr-Abl1 inhibitors to the extent that today it could be considered as manageable chronic disease. Although, ATP-competitive Bcr-Abl1 inhibitors set the milestone for treatment of CML, resistance on therapy in significant number of patients still remains major challenge. 3D quantitative structure-activity relationship (3D-QSAR) model of selected Bcr-Abl1 inhibitors was built in order to gain insight into structural requirements for inhibitory activity. The 3D-QSAR model with best validation parameters was selected for further study and design of novel inhibitors.14th International Conference on Fundamental and Applied Aspects of Physical Chemistry, September 24–28, 2018, Belgrad

Results of the recent excavations of the Late Neolithic Site Kotlina, Baranya county: flint, stone and bone artefacts

The site of Kotlina was discovered in 2009 during a large archaeology prospection project of Baranya in NE Croatia. Systematic archaeological research of the site was initiated in 2018 and the project “Kotlina, the prehistoric site” encompassed non-invasive field survey and excavations. Until today, the area covering approximately 150 m2 was excavated, revealing diverse archaeological structures: post holes, rubbish pits and graves. The portable archaeological finds were rich and diverse and consist of pottery sherds, animal bones, bone artefacts, chipped stone artefacts, polished and abrasive stone artefacts as well as one bead made from mollusc shell. Also, one very small fragment of copper and a large quantity of obsidian tools were discovered during the excavations. Portable archaeological finds and absolute dates show that the site can be attributed to the Late Neolithic Lengyel culture. In this paper, the results of the analysis of flint, stone and bone artefacts using variety of techniques that focus on the raw materials selection, technology and typology will be presented. Both local and imported raw materials were used, thus showing a complex pattern of raw material acquisition and management. The study of archaeological implements provided important information about the Lengyel communities located to the south of the Drava River. It will be very interesting to compare these data with other Lengyel sites in the north.[https://www.e-a-a.org/eaa2022/] [javascript://[Uploaded files/EAA 2022/EAA 2022 Abstract Book 27 August.pdf]

Using virtual software applications in education

Centar za permanentno obrazovanje kao deo Instituta za nuklearne nauke "Vinča" se dugi niz godina bavi dopunskim stručnim obrazovanjem i osposobljavanjem stručnjaka multidisciplinarnih profila koji u svom radu koriste radioaktivne materijale i generatore jonizujućih zračenja. Zajedničko za sve kurseve u Centru, osim visokog kvaliteta obuke, je i stalno praćenje i uvoĎenje savremenih i novih metoda edukacije. Sve veći razvoj informacionih tehnologija uticao je i na razvoj metoda i tehnika u samomobrazovanju. Najnoviji trend u edukaciji je da se realne laboratorije zamenjuju virtuelnim laboratorijama. Zaposleni u Centru za permanentno obrazovanje odlučili su da svoje kurseve oplemene i obogate primenom virtuelnih programskih aplikacija koje će pomoći da se polaznicima kurseva na jednostavan, lak i zabavan način približe osnovni pojmovi vezani za siguran i bezbedan rad sa radioaktivnim izvorima i generatorima jonizujućih zračenja poštujući sve mere zaštite od jonizujućih zračenja. U ovom radu prikazane su neke od virtuelnih aplikacija koje se koristeu okviru praktičnih vežbi na kursevima iz oblasti osnova radijacione fizike i osnovnih principa zaštite od jonizujućih zračenja.Center for permanent education (CPE) of the "Vinča" Institute of nuclear sciences is a unique educational institution in Serbia. The main objective of the CPE is an additional and complementary education of various profiles of specialists who use radioactive isotopes and sources of ionizing radiation in their everyday work. The CPE aims to improve the quality of its services and strives to continuously follow and introduce modern and new methods of education. The increasing development of information technologies has influenced the development of methods and techniques in the education itself. The latest trend in education is to replace the real laboratories with the virtual ones. The employees of the Center for Permanent Education have, therefore, decided to enrich the CPE courses with virtual software applications. Their use explains the basic concepts of ionizing radiation protection and safety in a simple, easy and fun way. In this paper, we describe some of the virtual software applications that are used in the laboratory curriculum of the CPE courses aimed at furthering education of professionals who work with radioactive sources and generators of ionizing radiation.Proceedings: [http://vinar.vin.bg.ac.rs/handle/123456789/8681]XXX симпозијум ДЗЗСЦГ (Друштва за заштиту од зрачења Србије и Црне Горе), 2- 4. октобар 2019. године, Дивчибаре, Србиј

Using virtual software applications in education

Centar za permanentno obrazovanje kao deo Instituta za nuklearne nauke "Vinča" se dugi niz godina bavi dopunskim stručnim obrazovanjem i osposobljavanjem stručnjaka multidisciplinarnih profila koji u svom radu koriste radioaktivne materijale i generatore jonizujućih zračenja. Zajedničko za sve kurseve u Centru, osim visokog kvaliteta obuke, je i stalno praćenje i uvoĎenje savremenih i novih metoda edukacije. Sve veći razvoj informacionih tehnologija uticao je i na razvoj metoda i tehnika u samomobrazovanju. Najnoviji trend u edukaciji je da se realne laboratorije zamenjuju virtuelnim laboratorijama. Zaposleni u Centru za permanentno obrazovanje odlučili su da svoje kurseve oplemene i obogate primenom virtuelnih programskih aplikacija koje će pomoći da se polaznicima kurseva na jednostavan, lak i zabavan način približe osnovni pojmovi vezani za siguran i bezbedan rad sa radioaktivnim izvorima i generatorima jonizujućih zračenja poštujući sve mere zaštite od jonizujućih zračenja. U ovom radu prikazane su neke od virtuelnih aplikacija koje se koristeu okviru praktičnih vežbi na kursevima iz oblasti osnova radijacione fizike i osnovnih principa zaštite od jonizujućih zračenja.Center for permanent education (CPE) of the "Vinča" Institute of nuclear sciences is a unique educational institution in Serbia. The main objective of the CPE is an additional and complementary education of various profiles of specialists who use radioactive isotopes and sources of ionizing radiation in their everyday work. The CPE aims to improve the quality of its services and strives to continuously follow and introduce modern and new methods of education. The increasing development of information technologies has influenced the development of methods and techniques in the education itself. The latest trend in education is to replace the real laboratories with the virtual ones. The employees of the Center for Permanent Education have, therefore, decided to enrich the CPE courses with virtual software applications. Their use explains the basic concepts of ionizing radiation protection and safety in a simple, easy and fun way. In this paper, we describe some of the virtual software applications that are used in the laboratory curriculum of the CPE courses aimed at furthering education of professionals who work with radioactive sources and generators of ionizing radiation.Proceedings: [http://vinar.vin.bg.ac.rs/handle/123456789/8681]XXX симпозијум ДЗЗСЦГ (Друштва за заштиту од зрачења Србије и Црне Горе), 2- 4. октобар 2019. године, Дивчибаре, Србиј

Achieved results in radiation protection education of the Center for Permanent Education INN “Vinča”

The Center for permanent education (CPE) of the “Vinča” Institute of nuclear sciences is a unique educational institution in Serbia. The main objective of the CPE is an additional and complementary education of various profiles of specialists who use radioactive isotopes and sources of ionizing radiation in their everyday work in accordance with the Law on Radiation and Nuclear Safety and Security of Serbia. Application of ionizing radiation in various fields of activity (industry, medicine, scientific research) carries the appropriate risks to the health and safety of those who work with ionizing radiation sources. It is therefore crucial that the handling of radioactive sources and ionizing radiation generators, as well as the handling of nuclear waste, be in line with certain safety standards aimed at protecting those exposed to ionizing radiation. Center for Permanent Education is authorized by the Directorate for Radiation and Nuclear Safety and Security of Serbia to provide additional training and education for occupationally exposed persons and persons responsible for implementing measures radiation protection. Accordingly, the activities of the Center are focused on the organization of different types of courses, depending on the category of a particular radiation activity. Permanent education program defines: additional and appropriate training before beginning work with radiation sources, training of persons responsible for implementing radiation protection measures, and periodic renewal of knowledge provided for all occupationally exposed persons at least once in five years. The special type of courses refers to courses for work with open sources of radiation, where longer and more detailed training is required. The aim of this work is to present the achieved results related to the number of held courses and the number of participants between 2011 and 2020, as well as years with the most and the least held courses and years with the largest and the smallest number of participants. These numbers have been changed in accordance with the requirements of legal regulations related to radiation protection. The Center for permanent education has worked since 1956, but information about all courses and candidates have been kept within database program only since 2011. During that period, 1503 different courses were held related to radiation protection. The most courses (272) were held in 2018 and the least (43) in 2011. Regarding the number of candidates, 5805 candidates completed the courses in the period between 2011 and to 2020. The largest number of candidates (1036) were in 2018, and the smallest (185) in 2013. The CPE aims to improve the quality of its services and strives to continuously follow and introduce modern and new methods of education for the next period.IX International Conference on Radiation in Various Fields of Research : RAD 2021 : book of abstracts; June 14-18, 2021; Herceg Novi, Montenegr

Optimization of ultrasound-assisted extraction of total polyphenolic compounds from Juglans nigra L. leaves

The ultrasound-assisted extraction of Juglans nigra L. leaves was optimized with respect to total phenolic content (TP) of the extracts by varying the concentration of aqueous ethanol solution (E) and different solvent-to-solid ratio (S). The influence and optimum of the operating parameters (E and S) was examined using response surface methodology (RSM). The statistical criteria indicated the adequacy, reliability and precision of the developed RSM model. RSM showed that maximum extraction yield of TP 28.59 mg g(-1) of draw plant was achieved at the optimal values of 50% E and 20 kg kg(-1) S. Using the modelled optimized conditions, the detected relative difference between the predicted and the experimental yield was +/- 2.3 %. The determined TP content in the extracts varied from 12.54 to 29.26 mg GAE g(-1) of dry plant indicated that J. nigra is a valuable source of phenolic substances. The extracts of J. nigra leaves obtained under optimal conditions showed good antioxidant activity (IC50 = = 18.91 +/- 0.03 mu g cm(-3)) which was determined by the scavenging effect on 2,2-diphenyl-1-picrylhydrazyl radical. The optimization of the TP extraction process is the important step in improving techno-economics of the potential commercial preparation of J. nigra extracts, as natural source of antioxidants

Kinetic profile of the antioxidant activity of propolis extract: 2,2-diphenyl-1-picrylhydrazyl radical bleaching assay

The objective of this paper was to assess the antiradical effectiveness of propolis extract (PE) based on 2.2-diphenyl-1-picrylhydrazyl radical (DPPH center dot) bleaching assay kinetic profile. The kinetic profile of scavenging DPPH center dot for PE exhibited one kinetic period characterized by one kinetic constant. The second-order rate constant (k(2)) for the oxidation of PE by DPPH center dot, determined for the first time in this study, was 0.17 dm(3)g(-1)s(-1). The obtained k(2) value was compared to that of synthetic antioxidants and natural extracts used in the food industry. Kinetic analysis of PE antiradical effectiveness showed that the k(2) was within the range values for natural colorants of fruit extracts and should be considered as a fast acting natural antioxidant source. The k(2) parameter indicates the extent of oxidation inhibition that is based on all of the kinetic profiles of DPPH bleaching rather than single point measurements. For this reason, the kinetic analysis should become a necessary step for more precise antioxidative characterization of propolis

Optimization of Ultrasound-Assisted Extraction of Total Extractive Substances from Galium verum L.

Ultrasound-assisted extraction (US-AE) of total extractive substances from the herb Lady`s Bedstraw (Galium verum L.) is useful extraction method for its application in food manufacturing. The effect of different temperatures (T) and solvent-to-solid ratios (S) on the US-AE extraction yield was studied in this paper. The influence and optimum of the operating parameters (T and S) was investigated using response surface methodology (RSM). The T and S had impact on extraction yield. RSM showed that the optimal extraction parameters, which gave a maximum extraction yield of 18.7%, were T of 40 oC and S of 30 kg kg-1. Using modeled optimized conditions, detected relative difference between predicted and experimental yield was 3.3 %. RSM successfully predicted optimal parameters and proved as useful tool for standardization of extraction conditions for bioactive components from plants important for food industry

Optimization of Ultrasound-Assisted Extraction of Total Extractive Substances from Galium verum L

Ultrasound-assisted extraction (US-AE) of total extractive substances from the herb Lady's Bedstraw (Galium verum L.) is useful extraction method for its application in food manufacturing. The effect of different temperatures (T) and solventto-solid ratios (S) on the US-AE extraction yield was studied in this paper. The influence and optimum of the operating parameters (T and S) was investigated using response surface methodology (RSM). The T and S had impact on extraction yield. RSM showed that the optimal extraction parameters, which gave a maximum extraction yield of 18.7%, were T of 40 oC and S of 30 kg kg(-1). Using modeled optimized conditions, detected relative difference between predicted and experimental yield was 3.3 %. RSM successfully predicted optimal parameters and proved as useful tool for standardization of extraction conditions for bioactive components from plants important for food industry

Chemical Composition and Protective Possibilities of Juglans Nigra Leaves and Green Husks Extracts: DNA Binding and Micronucleus Assay in Human Lymphocytes

To better understand the mechanism of action of the compounds in the ethanolic extracts of J. nigra leaves and green husks, their binding to CT-DNA was investigated. This study was conducted to elucidate the in vitro protective effect of extracts against chromosomal damage in mitogen-induced human lymphocytes and investigate the possible application of selec+ted extracts as a natural source of polyphenolic compounds. Using HPLC-MS analysis, 103 different compounds were identified as having a higher number of active species, which is consistent with their activity. The frequency of micronuclei (MN) was scored in binucleated cells, and the nuclear proliferation index was calculated. Cyclic voltammetry experiments demonstrate that the nature of the interaction between extracts and CT-DNA is a synergy of electrostatic and intercalative modes, where leaves extracts showed a higher ability to bind to DNA. Extracts showed excellent antioxidant activity. At a concentration of only 4 μg/mL, extract of J. nigra leaves and the green husks reduced the incidence of MN by 58.2% and 64.5%, respectively, compared to control cell cultures