Who Is Balancing: Is It RBC or Acid-Base Status?

Hemoglobin is an important intracellular protein buffer present inside the red blood cells (RBC). When the partial pressure of carbon dioxide (pCO2) is increased, it freely diffuses into the RBC where it reacts with water molecules to form carbonic acid which dissociates to form bicarbonate and hydrogen ions by the enzyme carbonic anhydrase. Hydrogen ions liberated in this reaction are buffered by hemoglobin. Oxyhemoglobin is a stronger acid than deoxyhemoglobin. Oxygenation of hemoglobin causes an increase in net titratable hydrogen ion due to the Haldane effect. As the oxygen saturation of hemoglobin (sO2) increases, the base excess is changed in the acidic direction, or as the sO2 decreases, the base excess is changed in alkaline direction. The changes in the level of the enzyme carbonic anhydrase in RBC are related to the changes in pH, pCO2, and bicarbonate levels in the blood. The understanding of the acid-base balance is a challenging task, but at the same time, it has immense clinical value. The relationship of carbonic anhydrase enzyme present inside the RBC in maintaining the acid-base balance to the commonly employed arterial blood gas (ABG) parameters like pH, pCO2 bicarbonate, and base excess may help us for better understanding

A study on micronucleus frequency in cervical smear as biomarker for genetic damage in polycystic ovarian syndrome

Background: Polycystic ovary syndrome (PCOS) is a multifactorial endocrine disorder with metabolic and reproductive consequences. Genomic damage and susceptibility to cancer are noteworthy concerns in PCOS. Relationship between PCOS and endometrial and ovarian cancer has already been established. The presence of high body mass index, excess triglycerides, oxidative stress and occurrence of metabolic syndrome is a frequent occurrence in PCOS. This may succeed into genetic damage and susceptibility to cervical cancer as they are also known risk factors of the same. The aim of the study was to estimate the frequency of micronuclei in cervical smear and to determine if it can be used as a biomarker of genomic instability and susceptibility to cervical changes in the future in PCOS.Methods: This observational case control study included 38 subjects diagnosed with PCOS by Rotterdam’s criteria and 38 controls and was conducted between September 2018 to March 2020 in VIMS and RC. Data regarding age and anthropometric details and cervical smear samples was collected from all the subjects. The frequency of micronuclei in cervical smears was expressed as mean±standard deviation (SD). Differences between the PCOS group and the control group were examined for statistical significance using two-sample independent t-test. A p value of ≤0.05 denoted statistically signifi­cant difference.Results: The mean±SD of micronuclei frequencies in cervical smears was observed to be 1.69±0.69 and 0.33±0.18 (p value <0.0001) in the subjects with PCOS and control group, respectively.Conclusions: Micronuclei frequency was found to be elevated in cervical smears of women with PCOS when compared to controls indicating genetic instability and probable susceptibility to cervical cancer in the future in women with PCOS.

The abdominal aorta and its branches: anatomical variations and clinical implications

Background: Vascular variations regarding the branching pattern of the aorta are important in different laparoscopic surgeries, liver and kidney transplantation, oncologic resections, and various interventional radiological procedures in the abdominal region. The present work was undertaken on cadavers to examine the prevalence of vascular patterns of the important branches of the abdominal aorta. Material and methods: A total of 50 properly embalmed and formalin fixed cadavers were dissected in the abdominal region, and viscera were mobilised to expose the origin of important branches of the abdominal aorta. Celiac trunk, superior mesenteric, inferior mesenteric, right and left renal, left and right gonadal arteries, and the division of the abdominal aorta into right and left common iliac arteries were observed regarding their level of origin and for presence of any anatomical variations. Results: The celiac trunk origin was located at the level of the T12 vertebra in 64% of cadavers, superior mesenteric at L1 in 76%, inferior mesenteric at L3 in 68%, left and right renal at L1 in 82% and 80%, respectively, and left and right gonadal at L2 in 84% and 86%, respectively; whereas the aortic bifurcation was most common at the level of the L4 vertebra in 54% of cadavers. Important anatomical variations were photographed. Conclusions: Defective fusion of the vitelline arteries during the embryonic stage resulted in the aforementioned anatomical variations. Knowledge of aortic variations is useful for appropriate radio diagnostic interventions and is helpful to decrease complications like vascular bleeding while ligating and anastomosing blood vessels, which is an integral part of many abdominal surgeries. (Folia Morphol 2011; 70, 4: 282&#8211;286

GIS-MAP based Spatial Analysis of Rainfall Data of Andhra Pradesh and Telangana States Using R

The rainfall conditions across wide geographical location and varied topographic conditions of India throw challenge to researchers and scientists in predicting rainfall effectively. India is Agriculture based country and it mainly depends on rainfall. Seasons in India are divided into four, which is winter in January and February, summer is from March to May, monsoon is from June to September and post monsoon is from October to December. India is Agriculture based country and it mainly depends on rainfall. It is very difficult to develop suitable rainfall patterns from the highly volatile weather conditions. In this Paper, it is proposed that Map based Spatial Analysis of rainfall data of Andhra Pradesh and Telangana states is made using R software apart from Hybrid Machine learning techniques. A Study will be made on rainfall patterns based on spatial locations. The Visual analytics were also made for effective study using statistical methods and Data Mining Techniques. This paper also introduced Spatial mining for effective retrieval of Remote sensed Data to deal with retrieval of information from the database and presents them in the form of map using R software

Anomalous origin of the left vertebral artery from the arch of the aorta: review of the literature and a case report

The present report describes an anomalous case of the left vertebral artery arising from the aortic arch between the left common carotid artery and the left subclavian artery in a male cadaver during dissection in an anatomical laboratory. Aortic origin of the vertebral artery is a rare anatomic variant. Detailed knowledge of anomalous origin is important for patients who undergo four-vessel angiography. Normally, the vertebral artery arises from the first part of the subclavian artery on both sides. We also review the anomalous origin of the vertebral artery in the literature and discuss its clinical significance. (Folia Morphol 2010; 69, 4: 258-260

Enhanced anti-corrosion characteristics of epoxy-functionalized graphene oxide nanocomposite coatings on mild steel substrates.

© 2024 The Authors. Published by Elsevier B.V. This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial 4.0 International License (CC BY-NC), https://creativecommons.org/licenses/by-nc/4.0/This experimental investigation focused on coating of functionalized graphene oxide (FGO) on the mild steel to minimize corrosion, using a new method. Prior to the coating, the fundamental chemical and morphological structures of FGO were studied with aid of some characterization techniques: X-ray diffraction (XRD), Raman, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Various epoxy/FGO coating formulations with 10, 20, 30, 40 and 50 ppm of FGO were prepared and coated on the mild steel substrate, adopting dip coating method. Immersion test was carried out to observe the corrosion inhibition efficiency of the graphene. The adsorption isothermal behavior was also studied using various models and compared with the data obtained from the experimental results. From the results obtained, the formation of irregular rough patches and separation of platelets like zebra lines indicated the formation of graphene nanoparticles, as observed through morphological analysis. The occurrence of sharp peak at 24.3◦ in the XRD pattern depicted the existence of graphene in nanoscale. Electrochemical impedance (EI) and potentiodynamic polarization (PP) studies re-ported the maximum corrosion inhibition efficiency of 87% at 50 ppm concentration of epoxy/FGO. It was evident that an increasing concentration of graphene exhibited significant corrosion resistance in both studies. The bonding energy of randomly oriented graphene platelets reduced the removal of metal, delaying the crack propagation in tortuous path on the coated surface. These results established the significance and applicability of this study in various relevant industries.Peer reviewe

Effect of Ayurvedic Treatment Modalities on Recurrent Pregnancy Loss

Recurrent pregnancy Loss is defined as the sequence of 2 or more spontaneous abortions as documented by either sonography or on histopathology before 20 weeks. It is a relatively common event, occurring in 15%-25% of pregnancies, and increasing in prevalence with maternal age. The causes of recurrent abortion are complex &amp; obscure. More than one factor may operate in a case. Identification and treatment of problems significantly increases the successful outcome in most cases. Recurrent pregnancy loss can be correlated with Puthraghni Yonivyapath and Garbhasravivandhya explained in Ayurvedic classics. Puthraghni is a clinical entity characterised by repeated pregnancy loss due to excessive intake of Rooksha Ahara and Vihara which results in repeated pregnancy losses. Ayurveda advises to do Shodhana Karma or purificatory therapies ending with Uttara Vasthi in recurrent losses. The study design was Prospective single arm interventional study conducted in the OPD and IPD of Govt. Ayurveda college hospital for Women and Children, Poojapura, Thiruvananthapuram with the study population of females of age group 20-38, diagnosed with RPL. IP management was done for 1 month followed by internal administration of Phala Sarpis as Vicharana Snehapana 10ml twice daily morning and evening ½ hour before food and Vilwadi Gulika 1 tab twice daily after food was also given for 2 months. After 15 months after the follow up period, Statistical analysis was done and Percentage of live births was assessed. Even though percentage of live births is 42.3, the success rate can be considered as 46.15% as the 1 patient to be delivered has completed 34 weeks of gestation and successfully continuing the pregnancy

Preparation and properties of cellulose / tamarind nut powder green composites

Using biopolymer cellulose as the matrix and tamarind nut powder (TNP) obtained from agricultural waste of tamarind nuts as the filler, the green composites were made. Cellulose was dissolved in environmental friendly solvent of aq. 8 wt. % Lithium hydroxide and 15 wt. % urea which was precooled to −12 ° C. To the cellulose solutions, TNP was added in 5 wt. % to 25 wt. % of cellulose separately. Each solution was evenly spread on glass plates and the wet composites were prepared by regeneration method using ethyl alcohol coagulation bath. The wet films were dried in air at room temperature. The dried composite films were characterized by FTIR spectroscopy, X-ray diffraction, thermogravimetric analysis and also tested for their tensile properties. The tensile strength and the % elongation at break of the composites were higher than those of the matrix and increased with TNP content. While the matrix had a tensile strength of 111.8 MPa, the cellulose/TNP composite loaded with 25 wt.% TNP possessed a tensile strength of 125.4 MPa (12% increase). Though the thermal stability of the composites was lower than cellulose matrix, all the composites were stable up to a temperature of 350 °C

Tensile and Flexural properties of MMT-clay/ Unsaturated Polyester using Robust Design Concept

Abstract. The effect of Nano-clay on the mechanical properties of Isophthalic unsaturated polyester was studied with the help of robust design Concept. Organo modified MMT nano-clay (Nanomer 1.31PS) was used as reinforcement. The weight percentage of nano-clay, impeller blade design, mixing hours and mixing speed were taken as control factors. In Taguchi design of experiments, L9 orthogonal array was employed to investigate the effect of control factors on mechanical properties such as tensile and flexural strength. X-ray diffraction (XRD) and Atomic force microscopy (AFM) results show the intercalation /exfoliation of clays in the polyester matrix