18 research outputs found

    Lamb Wave Mode Selection for Increased Sensitivity ot Interfacial Weaknesses of Adhesive Bonds

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    Interface quality between layers in a layered structure is critical in fracture and fatigue analysis. A theoretical and quantitative solution to the problem from a NDE point of view would be desirable in both manufacturing and for in-service investigation of a variety of different structures. For example a great need exists to develop a reliable and efficient inspection program of adhesive bond delamination and interfacial weakness detection in aging aircraft noting that the bond degradation generally preceeds cracking in the aluminum skin, starting at the rivet holes

    Development of sensitive polyclonal antibodies against dominant stored wheat grain fungus for its immunological detection: Presentation

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    Fungal infestation causes deterioration of stored food grains. Most fungal species produce secondary metabolites like aflatoxins which are highly toxic to animals and humans. Aspergillus flavus has been found to be the predominant contaminant in stored wheat grains collected from the godowns of Food Corporation of India, West Bengal. The present study focuses on the development of sensitive polyclonal antibodies (PAbs) for molecular immunological detection of dominant toxigenic fungus. Pure A. flavus isolate was cultured on coconut agar media and its spores were harvested and inactivated by 4% formaldehyde. The inactivated spores were injected into a rabbit along with Freund’s complete/incomplete adjuvant for the development of PAbs. Specificity of the raised antibodies in rabbit serum was examined by enzyme-linked immunosorbent assay (ELISA) using spore proteins as antigen obtained by bead beating method. Out of several proteins (ranging from 10 to 200 kDa present in spore, only two prominent proteins of around 76 kDa and 100 kDa were detected by western blot analysis using raised polyclonal antiserum. The PAbs were purified with protein A column followed by spore proteins conjugated CNBr activated sepharose column for its use in the detection of fungal antigens. This highly purified raised antibody can be used for the development of rapid, sensitive, and accurate techniques (such as dot blot/ELISA) for the detection of toxigenic fungi present in stored wheat grains.Fungal infestation causes deterioration of stored food grains. Most fungal species produce secondary metabolites like aflatoxins which are highly toxic to animals and humans. Aspergillus flavus has been found to be the predominant contaminant in stored wheat grains collected from the godowns of Food Corporation of India, West Bengal. The present study focuses on the development of sensitive polyclonal antibodies (PAbs) for molecular immunological detection of dominant toxigenic fungus. Pure A. flavus isolate was cultured on coconut agar media and its spores were harvested and inactivated by 4% formaldehyde. The inactivated spores were injected into a rabbit along with Freund’s complete/incomplete adjuvant for the development of PAbs. Specificity of the raised antibodies in rabbit serum was examined by enzyme-linked immunosorbent assay (ELISA) using spore proteins as antigen obtained by bead beating method. Out of several proteins (ranging from 10 to 200 kDa present in spore, only two prominent proteins of around 76 kDa and 100 kDa were detected by western blot analysis using raised polyclonal antiserum. The PAbs were purified with protein A column followed by spore proteins conjugated CNBr activated sepharose column for its use in the detection of fungal antigens. This highly purified raised antibody can be used for the development of rapid, sensitive, and accurate techniques (such as dot blot/ELISA) for the detection of toxigenic fungi present in stored wheat grains

    Effect of Geometric Parameters and Initial Imperfection on Global Buckling of Perforated Beams

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    This paper presents an extensive parametric study of elastic and inelastic buckling of cellular beams subjected to strong axis bending in order to investigate the effect of a variety of geometric parameters, and further generate mass data to validate and train a neural network-based formula. Python was employed to automate mass finite element (FE) analyses and reliably examine the influence of the parameters. Overall, 102,060 FE analyses were performed. The effects of the initial geometric imperfection, material nonlinearity, manufacture-introduced residual stresses, web opening diameter, web-post width, web height, flange width, web and flange thickness, end web-post width, and span of the beams and their combinations were thoroughly examined. The results are also compared with the current state-of-the-art design guidelines used in the UK. It was concluded that the critical elastic buckling load of perforated beams corresponds to the lateral movement of the compression flange while the most critical parameters are the web thickness and the geometry of the flange. However, from the inelastic analysis, the geometry and position of the web opening influence the collapse load capacity in a similar fashion to the geometry of the flange and thickness of the web. It was also concluded that the effect of the initial conditions was insignificant

    Elastic and inelastic buckling of steel cellular beams under strong-axis bending

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    This paper presents an extensive parametric study of elastic and inelastic buckling of cellular beams subjected to strong axis bending in order to investigate the effect of a variety of geometric parameters, and further generate mass data to validate and train a neural network-based formula. Python was employed to automate mass finite element (FE) analyses and reliably examine the influence of the parameters. Overall, 102,060 FE analyses were performed. The effects of the initial geometric imperfection, material nonlinearity, manufacture-introduced residual stresses, web opening diameter, web-post width, web height, flange width, web and flange thickness, end web-post width, and span of the beams and their combinations were thoroughly examined. The results are also compared with the current state-of-the-art design guidelines used in the UK. It was concluded that the critical elastic buckling load of perforated beams corresponds to the lateral movement of the compression flange while the most critical parameters are the web thickness and the geometry of the flange. However, from the inelastic analysis, the geometry and position of the web opening influence the collapse load capacity in a similar fashion to the geometry of the flange and thickness of the web. It was also concluded that the effect of the initial conditions was insignificant
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