Vibroacoustic performance of fiber metal laminates with delamination

In the present work, the numerical assessment of vibroacoustic (VA) performance of fiber metal laminates (FML) with mid-plane center delamination is presented. A fluid structure interaction study has been done using finite element method (FEM). Experimental validation is performed on an aluminium (AL) panel for verifying the correctness of finite element (FE) idealization procedure to simulate the fluid-structure interaction. Delamination is introduced in the FE model of FML panel and VA analysis is subsequently carried out. Sound transmission loss (STL) is computed on the panel with center delamination and without delamination. The overall sound pressure level (OASPL) shows that the presence of delamination (40% in total area) in FML has not changed the total energy of the transmitted sound when compared to aluminium and composites. However, in the narrow frequency bands (150–200 Hz, 200–250 Hz), the sound transmission nature has been significantly affected due to local delamination modes participating in the fluid-structure interaction process

Large patent ductus arteriosus in an adult complicated by pulmonary endarteritis and embolic lung abscess

Patent ductus arteriosus in the adult is an extremely rare clinical phenomenon. We report the case of a 34-year old man who developed pulmonary endarteritis and subsequent embolic lung abscess secondary to a large patent ductus arteriosus. This brief report also provides an overview of the natural history, potential complications, optimal therapy, and diagnostic dilemmas associated with this persistent congenital cardiac defect in adults

Development of a panel of recombinase polymerase amplification assays for detection of common bacterial urinary tract infection pathogens

Aims: To develop and evaluate the performance of a panel of isothermal real?time recombinase polymerase amplification (RPA) assays for detection of common bacterial urinary tract infection (UTI) pathogens. Methods and Results: The panel included RPAs for Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa and Enterococcus faecalis. All five RPAs required reaction times of under 12 min to reach their lower limit of detection of 100 genomes per reaction or less, and did not cross?react with high concentrations of nontarget bacterial genomic DNA. In a 50?sample retrospective clinical study, the five?RPA assay panel was found to have a specificity of 100% (95% CI, 78�0%) and a sensitivity of 89% (95% CI, 75�%) for UTI detection. Conclusions:The analytical and clinical validity of RPA for the rapid and sensitive detection of common UTI pathogens was established. Significance and Impact of the Study: Rapid identification of the causative pathogens of UTIs can be valuable in preventing serious complications by helping avoid the empirical treatment necessitated by traditional urine culture's 48�?h turnaround time. The routine and widespread use of RPA to supplement or replace culture?based methods could profoundly impact UTI management and the emergence of multidrug?resistant pathogens

Vibroacoustic Performance of Fiber Metal Laminates with Delamination

In the present work, the numerical assesment of vibroacoustic (VA) performance of Fibre Metal Laminates (FML) with mid-plane center delamination is presented. A fluid structure interaction study has been done using Finite Element Method (FEM). Experimental validation is performed on aluminium panel for verifying the correctness of Finite Element idealization procedure to simulate the fluid-structure interaction. Delamination is introduced in the FE model of FML panel and VA analysis is subsequently carried out. Sound Transmission Loss (STL) is computed on the panel with center delamination and without delamination. The Over All Sound Pressure Level (OASPL) shows that the presence of delamination (40% in total area) in FML has not changed the total energy of the transmitted sound, when compared to aluminium and composites. However, in the narrow frequency bands (150-200 Hz, 200-250 Hz) the sound transmission nature has been significantly affected due to local delamination modes, participating in the fluid-structure interaction process

Development of a Cytosensor for the Detection of Fusarium Oxysporum - A Functional Approach Towards Bioanalytical Applications

The development of bio-analytical methods for monitoring microorganisms have created opportunities for applications in biosensors, bioprocess monitoring, assessment of cell signalling, analysis of drug responses, among several others. The voltammetric sensing system employed for studying the electrode behavior of the fungus Fusarium oxysporum comprised working (gold) electrode (0.2 cm2 ) platinum as counter electrode (0.2 cm2 ) and a saturated calomel as the reference, where the electrochemical response corresponded to the growth phases (lag, log, stationary and decline) of the fungus. The electrochemical method based on voltammetric response matched well with the response obtained through conventional methodology, where the dry weight of the fungus is estimated against time. The peak potential is a function of scan rate, which is one of the characteristic features of a totally irreversible electrode process. It is important to mention here that this dependence is true regardless of reversibility for any diffusing redoxactive species. The proposed electrochemical method is less cumbersome and more accurate. Furthermore, the proposed electrochemical method captures the decline phase of fungal growth, which is generally difficult using the conventional method of assessment of the growth curve. Further experiments confirm that the anodic peaks were not due to the biomass or the fungal spores and only due to the extracellular metabolites. However, at this stage it is difficult to exactly determine the metabolite or the group of metabolites that are responsible for the anodic peak. In conclusion this cytosensor is capable of accurately and rapidly quantifying fungi with Fusarium oxysporum as a model organism

A low-cost smartphone-based platform for highly sensitive point-of-care testing with persistent luminescent phosphors

Through their computational power and connectivity, smartphones are poised to rapidly expand telemedicine and transform healthcare by enabling better personal health monitoring and rapid diagnostics. Recently, a variety of platforms have been developed to enable smartphone-based point-of-care testing using imaging-based readout with the smartphone camera as the detector. Fluorescent reporters have been shown to improve the sensitivity of assays over colorimetric labels, but fluorescence readout necessitates incorporating optical hardware into the detection system, adding to the cost and complexity of the device. Here we present a simple, low-cost smartphone-based detection platform for highly sensitive luminescence imaging readout of point-of-care tests run with persistent luminescent phosphors as reporters. The extremely bright and long-lived emission of persistent phosphors allows sensitive analyte detection with a smartphone by a facile time-gated imaging strategy. Phosphors are first briefly excited with the phone's camera flash, followed by switching off the flash, and subsequent imaging of phosphor luminescence with the camera. Using this approach, we demonstrate detection of human chorionic gonadotropin using a lateral flow assay and the smartphone platform with strontium aluminate nanoparticles as reporters, giving a detection limit of ?45 pg mL?1 (1.2 pM) in buffer. Time-gated imaging on a smartphone can be readily adapted for sensitive and potentially quantitative testing using other point-of-care formats, and is workable with a variety of persistent luminescent materials

Signaling network map of the aryl hydrocarbon receptor

We thank the Department of Biotechnology (DBT), Government of India for research support to the Institute of Bioinformatics, Bangalore. We thank the “Infosys Foundation” for research support to the Institute of Bioinformatics. We thank UK India Education and Research Initiative (UKIERI) for generous grant support. SDY is a recipient of DST-INSPIRE Senior Research Fellowship from Department of Science and Technology (DST), Government of India. AR and JA are recipients of Senior Research Fellowship from Council of Scientific and Industrial Research (CSIR), Government of India. RR is a recipient of Research Associateship from Department of Biotechnology, Government of India

Flotation Immunoassay: Masking the Signal from Free Reporters in Sandwich Immunoassays

In this work, we demonstrate that signal-masking reagents together with appropriate capture antibody carriers can eliminate the washing steps in sandwich immunoassays. A flotation immunoassay (FI) platform was developed with horseradish peroxidase chemiluminescence as the reporter system, the dye Brilliant Blue FCF as the signal-masking reagent, and buoyant silica micro-bubbles as the capture antibody carriers. Only reporters captured on micro-bubbles float above the dye and become visible in an analyte-dependent manner. These FIs are capable of detecting proteins down to attomole levels and as few as 106 virus particles. This signal-masking strategy represents a novel approach to simple, sensitive and quantitative immunoassays in both laboratory and point-of-care settings

NetPath: a public resource of curated signal transduction pathways

NetPath, a novel community resource of curated human signaling pathways is presented and its utility demonstrated using immune signaling data