Teknik Anyam dan Motif Dayak Ngaju pada Material Kulit untuk Produk Tas

Budaya adalah hal penting yang merupakan identitas. Indonesia memiliki budaya yang kaya dan beragam, salah satunya anyaman. Anyaman digunakan lebih dari ratusan tahun oleh masyarakat lokal di Indonesia khususnya di Kalimantan (Suku Dayak). Anyaman Dayak yang dibuat menggunakan bahan rotan atau bambu biasanya dipakai untuk membuat peralatan sehari-hari. Seiring dengan adanya perkembangan jaman, tradisi ini mulai memudar. Fashion adalah penanda identitas dan sebuah pelengkap tampilan fisik. Fashion sudah menjadi kebutuhan dengan berbagai macam jenis. Dalam era globalisasi, diperlukan kreativitas untuk membawa tradisi dalam bentuk modern yang diterima secara luas dalam masyarakat. Anyaman memiliki potensi yang luas untuk dibuat dengan material lain yang salah satunya adalah kulit. Kulit merupakan material yang berasal dari alam (hewan) dan sudah dipakai sejak awal peradaban manusia menjadi bahan dasar dari barang-barang sehari-hari. Kulit merupakan material yang mudah diolah. Aplikasi teknik anyaman Dayak pada material kulit ini diharapkan dapat mengangkat kembali tradisi anyaman pada dunia modern dalam bentuk produk dengan kualitas yang baik

An overview of real-time quantitative PCR: Principles and formats for environmental microbiology studies

© Global Science Publications. A fluorescence-based real-time quantitative polymerase chain reaction (qPCR) is a powerful and commonly used molecular technique for quantifying the rRNA or DNA of targeted organisms in environmental samples. qPCR assays are easy to perform, making them capable of high throughput and can combine high sensitivity with reliable specificity. qPCR analysis is the combination of the traditional endpoint PCR attached with fluorescents to record the accumulation of the amplicons in real time during each cycle of the PCR. Detection of amplicons during the early exponential phase enables quantification of the gene numbers because they are proportional to the starting template. This review is focussed on currently used qPCR platforms, the chemistries involved in real-time PCR systems mainly applied for the environmental microbiology studies. The various factors affecting quantification of environmental microbial communities using qPCR have also been discussed

Applications of real-time quantitative PCR for environmental microbiology studies

© Global Science Publications. The real-time quantitative polymerase chain reaction (qPCR) is a robust and sensitive molecular technique to quantify phylogenetic and key functional genes of microbial populations in various natural and engineered environmental ecosystems under varying physical and chemical environmental conditions. The generated quantitative qPCR data can be used to relate variation in abundance of specific microbial groups, species, family or functional gene of interest and levels of gene expression in comparison with the change in environmental factors and or system processes. In this review, several applications of qPCR for the understanding of environmental microorganisms have been presented. The main qPCR applications reviewed are: quantification of uncultured environmental bacteria, detection of pathogens in environmental samples, the effect of antibiotics on anaerobic digester microbial community and understanding mechanism of nitrification and ammonia metabolism in engineered environmental ecosystems

Pelaksanaan Pembelajaran dalam Memahami Kosakata Benda di Masa Pandemi Pada Siswa Tunarungu Kelas 2

This study aims to determine the description of the implementation in understanding the vocabulary of object for deaf children at SLB Negeri Bekasi Jaya. This research uses a qualitative approach with descriptive methods. The data used in this study swew obtained through speaches from the informants and actions observations in the field. Data collection techniques used are by conducting observations in the field,I nterviews with class teachers and document study. The findings obtained in this study are schools apply learning in understanding the vocabulary of object it’s using the vocabulary card.The strategy used by teacher  is ekspository strategy and using the question and answer method. The learning program be adapted based on the results of the assessment carried out on new student admissions. The beginning students mentioning the teachers words, then students classification vocabulary of object, and pair the vocabulary of object. Students are understanding the vocabulary of object when students can pair vocabulary of object card images with their own writing oneself without the help of parents. Supporting factors in understanding the vocabulary of object is students can be conducive and good parental cooperation. The obstacle experienced by the teachers its students easily lose focus during distance learning.Penelitian ini bertujuan untuk mengetahui gambaran pelaksanaan dalam memahami kosakata benda pada siswa tunarungu di SLB Negeri Bekasi Jaya. Penelitian ini menggunakan kualitatif dengan metode deskriptif. Data yang digunakan dalam penelitian ini diperoleh melalui ucapan dari narasumber dan tindakan yang diamat di lapangan melalui daring. Teknik pengumpulan data yang digunakan yaitu dengan melakukan observasi di lapangan, wawancara dengan guru kelas dan studi dokumen. Hasil temuan yang didapatkan dalam penelitian ini adalah sekolah menerapkan pembelajaran dalam memahami kosakata benda yaitu menggunakan kartu kosakata. Strategi yang digunakan oleh guru  yaitu strategi ekspositori dengan menggunakan metode tanya jawab dan pemberian tugas. Program pembelajaran disesuaikan berdasarkan hasil asesmen yang dilakukan pada penerimaan siswa baru. Langkah-langkah pelaksanaannya dimulai dengan siswa menyebutkan kosakata benda yang diucapkan oleh guru lalu mengklasifikasi kosakata benda dan memasangkan kosakata benda. Siswa dikatakan memahami kosakata benda bila siswa dapat memasangkan gambar kartu kosakata dengan tulisannya secara sendiri tanpa bantuan orangtua. Faktor pendukung dalam memahami kosakata benda yaitu siswa dapat kondusif dan kerjasama orang tua yang baik. Kendala yang dialami guru adalah siswa yang mudah kehilangan fokus saat pembelajaran jarak jauh.&nbsp

Use of Desipramine for the Treatment of Overactive Bladder Refractory to Antimuscarinic Therapy

Purpose: To evaluate the use of desipramine in the treatment of overactive bladder (OAB). Materials and Methods: We retrospectively evaluated 43 patients who were treated with desipramine for OAB refractory to antimuscarinic therapy. These OAB patients were stratified by the presence or absence of bladder pain. Results: Forty-three patients were evaluated with a mean follow up time of 12.2 +/- 4.6 months. The mean age of the patients was 71 16 years. Twelve patients (28%) discontinued desipramine, 9 due to perceived lack of efficacy, 2 due to central anticholinergic side effects, and 1 due to the development of oropharyngeal sores. Patients were stratified into two subgroups based upon treatment with desipramine for OAB alone (n = 29) or OAB and bladder pain (n = 14). There was no difference between the groups in regard to sex (P = .34), prior history of radiation (P = .19), side effects (P = .16), and specifically evaluated central anti-cholinergic side effects (P = .66). There was no statistical difference in the self-reported success rate of the medication (P = .48). In the OAB plus bladder pain subgroup, 71% of patients reported improvement in their pain. Overall, 13 (30%) patients had history of prior pelvic radiation and 10 of those (77%) reported improvement with desipramine. Conclusion: Desipramine is a potential useful treatment for patients with OAB. In addition, it can be used in patients with OAB and bladder pain and patients with complex OAB such as OAB caused by pelvic radiation

Algorithm for Training a Recurrent Multilayer Perceptron

An improved algorithm has been devised for training a recurrent multilayer perceptron (RMLP) for optimal performance in predicting the behavior of a complex, dynamic, and noisy system multiple time steps into the future. [An RMLP is a computational neural network with self-feedback and cross-talk (both delayed by one time step) among neurons in hidden layers]. Like other neural-network-training algorithms, this algorithm adjusts network biases and synaptic-connection weights according to a gradient-descent rule. The distinguishing feature of this algorithm is a combination of global feedback (the use of predictions as well as the current output value in computing the gradient at each time step) and recursiveness. The recursive aspect of the algorithm lies in the inclusion of the gradient of predictions at each time step with respect to the predictions at the preceding time step; this recursion enables the RMLP to learn the dynamics. It has been conjectured that carrying the recursion to even earlier time steps would enable the RMLP to represent a noisier, more complex system

Determination of Andrographolide Isolate Activity to α-Amylase and α-Glucosidase Using Apostolidis and Mayur Method

Disorders of carbohydrate metabolism can lead to diabetes mellitus. Carbohydrates are metabolized in the gastrointestinal tract into simple glucose and absorbed into the bloodstream and affected blood glucose levels. The absorption process is catalyzed by α- 1 ,4 - glycoside breaking bond enzyme , namely α - amylase and α -1 ,6 - glycoside breaking bond enzyme, namely α – glucosidase. They are found in the intestinal cells. Research had been conducted in an effort to develop an alternative treatment of diabetes mellitus by testing the ability of isolates of andrographolide in inhibiting α-amylase activity and α-glucosidase in vitro. Andrographolide isolates showed fairly good activity in inhibiting α-amylase ( IC50 = 1,.49 mg/mL) and weak in inhibiting α-glucosidase (IC50 = 38,86 mg/mL). Inhibition of α-amylase activity is evidence of one mechanism of andrographolide in reducing carbohydrate metabolism that can affect blood glucose levels and indicates that andrographolide is a potential alternative medicine in addressing diabetes mellitus

Assessing methanogenic archaeal community in full scale anaerobic sludge digester systems in Dubai, United Arab Emirates

© 2018 Khan et al. Introduction: Anaerobic digestion for methane production comprises of an exceptionally diverse microbial consortium, a profound understanding about which is still constrained. In this study, the methanogenic archaeal communities in three full-scale anaerobic digesters of a Municipal Wastewater Treatment Plant were analyzed by Fluorescence in situ hybridization and quantitative real-time Polymerase Chain Reaction (qPCR) technique. Methods & Materials: Fluorescence in situ hybridization (FISH) was performed to detect and quantify the methanogenic Archaea in the sludge samples whereas qPCR was carried out to support the FISH analysis. Multiple probes targeting domain archaea, different orders and families of Archaea were used for the studies. Results and Discussion: In general, the aceticlastic organisms (Methanosarcinaceae & Methanosaetaceae) were more abundant than the hydrogenotrophic organisms (Methanobacteriales, Methanomicrobiales, Methanobacteriaceae & Methanococcales). Both FISH and qPCR indicated that family Methanosaetaceae was the most abundant suggesting that aceticlastic methanogenesis is probably the dominant methane production pathway in these digesters. Conclusion: Future work involving high-throughput sequencing methods and correlating archaeal communities with the main operational parameters of anaerobic digesters will help to obtain a better understanding of the dynamics of the methanogenic archaeal community in wastewater treatment plants in United Arab Emirates (UAE) which in turn would lead to improved performance of anaerobic sludge digesters

Bacterial community structure in anaerobic digesters of a full scale municipal wastewater treatment plant - Case study of dubai, united arab emirates

© 2019, International Centre for Sustainable Development of Energy, Water and Environment Systems SDEWES. All rights reserved. A highly complex microbial community involved in anaerobic sludge digesters plays vital roles in sludge treatment. The data on microbial ecology is important to accomplish efficient operation of the anaerobic digesters. This study is aimed at monitoring the bacterial community of three full-scale anaerobic digesters of a full-scale municipal wastewater treatment Plant in Dubai, United Arab Emirates. Fluorescent in-situ hybridization technique was applied to identify the bacterial groups and quantitative polymerase chain reaction to compare the richness of bacterial and archaeal domain. Results of the fluorescent in-situ hybridization technique analysis showed that the phylum Proteobacteria was most abundant followed by cytophage-Flavobacterium group of Bacteroides, Firmicutes and Actinobacteria. Among proteobacterial subclass Delta- and Alpha- were dominating than Gamma- and Beta-proteobacteria. The genus Desulfobacter and Desulfobacterium were the dominant groups hybridizing 70-76% of total 4\u27, 6\u27- diamidino - 2 phenylindole stained cells. The quantitative polymerase chain reaction results showed that Bacterial domain was dominating in all three digesters compared to the archaeal domain