Flora Mediterranea

This peer-reviewed journal, published annually on behalf of OPTIMA, includes papers dealing with the study of biogeography, floristics and systematic botany in the Mediterranean area

Wood-decay fungi on trees of the city of Palermo (Sicily, Italy)

Nineteen taxa, belonging to 15 genera, included in 12 families of Basidiomycetes were observed on different living trees and stumps. Data on hosts and collection sites are reported together with notes on the causes of wood decay fungi attack and suggestions for solutions

Results of the 12th “Iter Mediterraneum” in Tunisia, 24 March - 4 April, 2014

Are here presented the Results of the 12th “Iter Mediterraneum” in Tunisia, 24 March - 4 April, 2014. They include: The organization and logistics of the 12th OPTIMA Iter in Tunisia by Domina & al.; a bioclimatic and vegetation overview of the studied areas by Smaoui; the Checklist of the vascular plants collected by Greuter & Domina; a first Checklist of the Bryophytes collected by Campisi & al; a first Checklist of the lichens collected by Guttová & al

Plant Extracts as Antimicrobial Agents in Sustainable Conservation of Erythrina caffra (Fabaceae) Historical Trees

Microbial colonization plays a relevant role in the biodegradation and biodeterioration of cultural and natural heritage, representing a revealing problem in conservation strategy. In this study, the essential oil (EO) and hydro-alcoholic extract (HAE) of Origanum vulgare L. (Lamiaceae), an aromatic perennial plant, representative of the Mediterranean basin, growing spontaneously and cultivated all over the world, were analysed. Natural products, such as essential oil and hydroalcoholic extract, have strong antiseptic and antimicrobial properties and are ad hoc applied for the sustainable conservation of Erithryna caffra (Fabaceae). The main taxa revealed in the damaging of these arboreal heritage, are Bacillus sp., Streptomyces sp. and Terribacillus sp. (as bacteria), Alternaria sp., Aspergillus sp. and Chaetomium sp. (as fungi). GS-MS analysis identified carvacrol, thymol and their biosynthetic precursors g-terpinene and p-cymene, as main components, and the antimicrobial efficiency assayed by in vitro methods (Agar Dish Diffusion, Well Plate Diffusion). In this study, by combining the application/exposure of both HAE and EO, the bacterial and fungal colonies development has been in vitro countered. The results confirm the possible use of plant products as a valid alternative to the traditional synthetic chemical biocides, with full respect to the environment

Species and habitat biodiversity measure and conservation at different scale in small Mediterranean islands

Small islands are geographically and ecologically well-defined areas in which biological processes are easier to schematize than in the mainland. There is a vast range of biodiversity measures, due to the large concept of biodiversity and the range of spatial, temporal and taxonomic scales used. Many existing measures are well designed and informative. But, very often they are inadequate for purposes beyond those for which they were specifically designed. Knowledge on trends in biodiversity loss is hindered by the absence of reliable basic data for most groups of organisms as well as habitats. Plants are primary producers and key structural elements for most ecosystems and islands are among the best floristically known territories in the Mediterranean. Large-scale habitat measurements have been aided greatly by advances in remote sensing and GIS software. However, the degree of resolution of this technique is still not adequate for many purposes, such as monitoring many habitats that occurs on the islands (e.g. temporary Mediterranean pools or ephemeral therophytic grasslands). The measure of population size is not practical for many taxa above all in islands that often have sectors of difficult access (e.g. sea cliffs). Lists and distribution mapping of taxa are probably the most commonly used surrogate for overall biodiversity at both local and broader scales. The species level is an accepted standard, because the concept of species is well understood also by the public, and policy makers. Information on the presence of higher plants is available for many Mediterranean islands due to records of visiting naturalists, and formal surveys undertaken by governments and NGOs (e.g. the PIM initiative). In addition, in the islands the lack of records of a species is more easily correlated to its disappearance from that territory than it is in the mainland. Anyway, particular attention has to be put on measurements of rarity and extinction risk. Most extinctions, after a first peak due to a specific phenomenon, have a long after-effects, whereby the species may persist at low numbers with a negligible chance of recovery and a severely diminished role in the ecosystem

SF3B1-mutated chronic lymphocytic leukemia shows evidence of NOTCH1 pathway activation including CD20 downregulation

Chronic lymphocytic leukemia (CLL) is characterized by a low CD20 expression, in part explained by an epigenetic-driven downregulation triggered by mutations of the NOTCH1 gene. In the present study, by taking advantage of a wide and well-characterized CLL cohort (n=537), we demonstrate that CD20 expression is downregulated in SF3B1-mutated CLL in an extent similar to NOTCH1-mutated CLL. In fact, SF3B1-mutated CLL cells show common features with NOTCH1-mutated CLL cells, including a gene expression profile enriched of NOTCH1-related gene sets and elevated expression of the active intracytoplasmic NOTCH1. Activation of the NOTCH1 signaling and down-regulation of surface CD20 in SF3B1-mutated CLL cells correlate with over-expression of an alternatively spliced form of DVL2, a component of the Wnt pathway and negative regulator of the NOTCH1 pathway. These findings are confirmed by separately analyzing the CD20-dim and CD20-bright cell fractions from SF3B1-mutated cases as well as by DVL2 knock-out experiments in CLL-like cell models. Altogether, the clinical and biological features that characterize NOTCH1-mutated CLL may also be recapitulated in SF3B1-mutated CLL, contributing to explain the poor prognosis of this CLL subset and providing the rationale for expanding novel agents-based therapies to SF3B1-mutated CLL

Wild food plants of popular use in Sicily

In the present work the authors report the result of their food ethnobotanical researches, which have been carried out in Sicily during the last thirty years. Data concerning 188 wild species used in the traditional Sicilian cuisine are reported. The authors underline those species that are partially or completely unknown for their culinary use and they illustrate other species that local inhabitants suggested in the prevention or treatment of symptomatologies caused by a refined diet, poor in vegetables. These data want to contribute to avoid the loss of traditional knowledge on uses and recipes concerning wild food botanicals, and to encourage further studies for those species that have not yet been sufficiently researched in their food chemical and nutritional profile. These studies may also suggest new applications for a few botanicals in medico-nutritional fields. The work includes also a short review of the seaweeds and mushrooms traditionally gathered and consumed in Sicily

Interactions between microenvironment and cancer cells in two animal models of bone metastasis

The preferential proliferation of cancer cells in the bone microenvironment is poorly characterised. Expression pattern of bone marrow and other organ microenvironment in contact with osteolytic (Walker W256) and osteoblastic (MatLyLu MLL) metastases were investigated. Fisher and Copenhagen rats received, respectively, W256 and MLL cells injection. Bone and soft tissues were analysed by immunochemistry for DKK1, cathepsin K, RANKL, MCSF or IL6 expression. Tartrate-resistant acid phosphatase (TRAcP)-positive cells were detected by a histoenzymatic technique. In bone, expressions of MCSF and DKK1 were shown in stromal cells of the bone marrow, in contact with metastatic foci of both tumours. Many stromal cells were found RANKL positive in the vicinity of the tumours. Cells expressing cathepsin K and multinucleated TRAcP+ cells were found in direct contact with trabeculae but also in bone marrow spaces near metastatic cells. In extraosseous tumours, cells in contact with malignant cells did not expressed DKK1, MCSF, cathepsin K and IL6. Some RANKL+ cells were found in the periphery of subcutaneous tumours but may represent Langerhans cells. Abnormal presence of TRAcP+ cells was never observed in the vicinity of malignant cells. Interaction between stromal and cancer cells induces the expression on the formers of characteristics leading to osteoclastogenesis only in the bone microenvironment