Global heat flow trends resolved from multiple geological and geophysical proxies

International audienceBecause global coverage of heat flow measurements is still poor in many areas, empirical estimators based on the geology, the thermotectonic age or the velocity structure of the upper mantle have often been used to affect an estimate to regions where such measurements are absent. On the basis of the assumption that heat flow is strongly related to its geodynamic environment, one may integrate multiple proxies derived from a large body of global geo- logical and geophysical data sets assembled during the past decades; these should help to better capture the variety of present-day settings. This idea is illustrated through two simple empirical methods: both of them are based on a set of examples, where heat flow measure- ments are associated with relevant terrestrial observables such as surface heat production, upper-mantle velocity structure, tectono-thermal age, on a 1◦ × 1◦ grid. To a given target point owning a number of observables, the methods associate a heat flow distribution rather than a deterministic value to account for intrinsic variability and uncertainty within a defined geodynamic environment. The 'best combination method' seeks the particular combination of observables that minimizes the dispersion of the heat flow distribution generated from the set of examples. The 'similarity method' attributes a weight to each example depending on its degree of similarity with the target point. The methods are transparent and are able to handle sets of observables that are not available over the whole Earth (e.g. heat production). The resulting trends of the mean heat flow deduced from the two methods do not differ strongly, but the similarity method shows a better accuracy in cross-validation tests. These tests suggest that the selected proxies have the potential to recover at least partly medium- to large-scale features of surface heat flow. The methods depict the main global trends of low heat flow in stable and ancient regions, and thermal high in active orogens and rift zones. Broad thermal anomalies are outlined in the Sahara and in the tectonically active eastern part of Antarctica. The similarity method estimates a continental heat loss of 13.6 ± 0.8 TW (2σ uncertainty), which is consistent with previous estimates

Statistical modeling of the space–time relation between wind and significant wave height

Many marine activities, such as designing ocean structures and planning marine operations, require the characterization of sea-state climate. This study investigates the statistical relationship between wind and sea states, considering its spatiotemporal behavior. A transfer function is established between wind fields over the North Atlantic (predictors) and the significant wave height (predictand) at three locations: southwest of the French coast (Gironde), the English Channel, and the Gulf of Maine. The developed method considers both wind seas and swells by including local and global predictors. Using a fully data-driven approach, the global predictors' spatiotemporal structure is defined to account for the non-local and non-instantaneous relationship between wind and waves. Weather types are constructed using a regression-guided clustering method, and the resulting clusters correspond to different wave systems (swells and wind seas). Then, in each weather type, a penalized linear regression model is fitted between the predictor and the predictand. The validation analysis proves the models skill in predicting the significant wave height, with a root mean square error of approximately 0.3 m in the three considered locations. Additionally, the study discusses the physical insights underlying the proposed method.</p

Modifying the stereochemistry of an enzyme-catalyzed reaction by directed evolution

Aldolases have potential as tools for the synthesis of stereochemically complex carbohydrates. Here, we show that directed evolution can be used to alter the stereochemical course of the reaction catalyzed by tagatose-1,6-bisphosphate aldolase. After three rounds of DNA shuffling and screening, the evolved aldolase showed an 80-fold improvement in k-cat/K-m toward the non-natural substrate fructose 1,6-bisphosphate, resulting in a 100-fold change in stereospecificity. (31)P NMR spectroscopy was used to show that, in the synthetic direction, the evolved aldolase catalyzes the formation of carbon—carbon bonds with unnatural diastereoselectivity, where the >99:<1 preference for the formation of tagatose 1,6-bisphosphate was switched to a 4:1 preference for the diastereoisomer, fructose 1,6-bisphosphate. This demonstration is of considerable significance to synthetic chemists requiring efficient syntheses of complex stereoisomeric products, such as carbohydrate mimetics

A Study of Nuclear Transcription Factor-Kappa B in Childhood Autism

BACKGROUND: Several children with autism show regression in language and social development while maintaining normal motor milestones. A clear period of normal development followed by regression and subsequent improvement with treatment, suggests a multifactorial etiology. The role of inflammation in autism is now a major area of study. Viral and bacterial infections, hypoxia, or medication could affect both foetus and infant. These stressors could upregulate transcription factors like nuclear factor kappa B (NF-κB), a master switch for many genes including some implicated in autism like tumor necrosis factor (TNF). On this hypothesis, it was proposed to determine NF-κB in children with autism. METHODS: Peripheral blood samples of 67 children with autism and 29 control children were evaluated for NF-κB using electrophoretic mobility shift assay (EMSA). A phosphor imaging technique was used to quantify values. The fold increase over the control sample was calculated and statistical analysis was carried out using SPSS 15. RESULTS: We have noted significant increase in NF-κB DNA binding activity in peripheral blood samples of children with autism. When the fold increase of NF-κB in cases (n = 67) was compared with that of controls (n = 29), there was a significant difference (3.14 vs. 1.40, respectively; p<0.02). CONCLUSION: This finding has immense value in understanding many of the known biochemical changes reported in autism. As NF-κB is a response to stressors of several kinds and a master switch for many genes, autism may then arise at least in part from an NF-κB pathway gone awry

Intramolecular Epistasis and the Evolution of a New Enzymatic Function

Atrazine chlorohydrolase (AtzA) and its close relative melamine deaminase (TriA) differ by just nine amino acid substitutions but have distinct catalytic activities. Together, they offer an informative model system to study the molecular processes that underpin the emergence of new enzymatic function. Here we have constructed the potential evolutionary trajectories between AtzA and TriA, and characterized the catalytic activities and biophysical properties of the intermediates along those trajectories. The order in which the nine amino acid substitutions that separate the enzymes could be introduced to either enzyme, while maintaining significant catalytic activity, was dictated by epistatic interactions, principally between three amino acids within the active site: namely, S331C, N328D and F84L. The mechanistic basis for the epistatic relationships is consistent with a model for the catalytic mechanisms in which protonation is required for hydrolysis of melamine, but not atrazine

Geometric morphometrics as a tool for identifying emperor fish (Lethrinidae) larvae and juveniles

The aim of this study was to evaluate the efficiency of geometric morphometrics for describing the body shape of fish larvae and juveniles, and identifying them to species, in comparison with traditional linear measurements. Species of emperor fishes (Perciformes: Lethrinidae, genus Lethrinus) were chosen as the model group, as the late larval and early juvenile stages in this genus are particularly difficult to identify. Forty-five individuals of different species of Lethrinus were collected from the south-western lagoon of New Caledonia between May 2005 and March 2006. The individuals were first identified to species by their partial cytochrome-b gene sequence. They were then morphologically characterized using eight linear measurements and 23 landmarks recorded on digital photographs. Except for a small proportion of individuals, geometric morphometrics gave better results to distinguish the different species than linear measurements. A 'leave one out' approach confirmed the nearly total discrimination of recently settled Lethrinus genivittatus and Lethrinus nebulosus, whereas traditional identification keys failed to distinguish them. Therefore, geometric morphometrics is a promising tool for identifying fish larvae and juveniles to species. An effective approach would require building image databases of voucher specimens associated with their DNA barcodes. These images could be downloaded by the operator and processed with the specimens to be identified

Datenbanksystem zur Bestandesbetreuung von Milchviehherden mit Schwerpunkt auf der Eutergesundheit

In der Michviehhaltung ist die integrierte tierärztliche Bestandsbetreuung (ITB) eine wirksame Methode, um Gesundheitsprobleme wie Mastitis und Fruchtbarkeitssörungen langfristig zu bekämpfen. Um den Zeitaufwand und die Kosten für die ITB zu optimieren, müssen entsprechende technische Hilfmittel vorhanden sein, die eine schnelle und komfortable Datenverarbeitung gewährleisten und praxistaugliche Beratungstools zu Verfügung stellen. Das Forschungsinstitut für biologischen Landbau (FiBL) hat ein solches Datenbanksystem für das Bestandsbetreuungsprojekt "Pro-Q" entwickelt. Ziel der Datenbank ist, extern zur Verfügung stehende und selbst erhobene Daten zu gebündelten Informationen auf Herden- und Einzeltierebene (inkl. Euterviertelebene) zusammenzubringen und Landwirt, Tierarzt und Berater regelmässig zur Verfügung zu stellen. Diese erhalten so einen umfassenden Überblick über die Gesundheit der Herde und des Einzeltieres. Die Datenbank erlaubt den gleichzeitigen Zugriff durch mehrere Benutzer. Fernzugriff via Internet ist ebenfalls möglich. In diesem Beitrag sollen die wesentlichen Komponenten im Hinblick auf die Tauglichkeit in praxi dargelegt werden