Effects of genistein on melanosis and microbial quality of Litopenaeus vannamei during ice storage

Utilization of genistein as a natural inhibitor was studied alone and in combination with conventional treatments on melanosis and microbial quality of fresh L. vannamei during 10 days ice storage. Treatments were as followed: A (dipping samples in distilled water), B (dipping in 1.25 % sodium metabisulphite solution), C (dipping in 0.01 % genistein + 2% glycerol solution), D (dipping in 0.1 % genistein + 2% glycerol solution), E (dipping in 1% NaCl + 0.05 % EDTA + 0.5 % ascorbic acid + 0.5 % lactic acid solution), F (dipping in 0.01% genistein + 2 % glycerol + 1 % NaCl + 0.05 % EDTA + 0.5 % ascorbic acid + 0.5 % lactic acid solution) and G (dipping in 0.1 % genistein + 2 % glycerol + 1 % NaCl + 0.05 % EDTA + 0.5 % ascorbic acid + 0.5 % lactic acid solution). Results showed that G treatment was more affective on inhibiting of melanosis in L. vannamei. Mesophilic and psycrophilic bacterial counts in G treatment were lower than other treatments during ice storage

Severe Iatrogenic Calcinosis Cutis From Extravasated Calcium Gluconate

Iatrogenic calcinosis cutis occurs when insoluble calcium salts deposit in cutaneous and subcutaneous tissue. Iatrogenic calcinosis cutis is a rare complication from a variety of medical interventions, most commonly due to extravasated intravenous calcium-containing solutions. We present a severe case of iatrogenic calcinosis cutis in a patient with end-stage renal disease and an elevated serum calcium-phosphate product. Iatrogenic calcinosis cutis has a wide range of clinical presentations. Either subclinical or clinically noticeable extravasations may cause mild to severe calcinosis cutis. Patients with increased serum calcium and phosphate may be at increased risk of iatrogenic calcinosis cutis. Treatment options include conservative, pharmacologic, or surgical management

Sensible Heat Balance Estimates of Transient Soil Ice Contents

Soil ice content is an important component for winter soil hydrology. The sensible heat balance (SHB) method using measurements from heat pulse probes (HPPs) is a possible way to determine transient soil ice content. In a previous study, in situ soil ice content estimates with the SHB method were inaccurate, due to thermal conductivity errors and the use of relatively long time steps for calculations. The objective of this study is to reexamine the SHB method for soil ice content determination. A soil freezing and thawing laboratory experiment was performed with soil columns and heat exchangers. Transient soil ice contents in the soil columns during soil freezing and thawing were determined with the SHB method. The SHB method was able to determine dynamic changes in soil ice contents during initial freezing and final thawing for soil temperatures between −5 and 0°C when latent heat values associated with ice formation or with thawing were relatively large. During an extended freezing period, when soil temperatures were below −5°C, the small associated latent heat fluxes were below the sensitivity of the SHB method, and the SHB method did not provide accurate estimates of ice contents with time. However, the soil ice contents during the extended freezing period could be estimated well from changes in volumetric heat capacity (C) determined with HPP. Thus, combining the SHB method for initial freezing and final thawing, with a change in Cmethod for extended freezing periods, allowed determination of dynamic soil ice contents for the entire range of freezing and thawing soil temperatures investigated. HPPs were able to measure soil ice contents

Mechanisms of the Epithelial-Mesenchymal Transition and Tumor Microenvironment in Helicobacter pylori-Induced Gastric Cancer

Helicobacter pylori (H. pylori) is one of the most common human pathogens, affecting half of the world's population. Approximately 20% of the infected patients develop gastric ulcers or neoplastic changes in the gastric stroma. An infection also leads to the progression of epithelial-mesenchymal transition within gastric tissue, increasing the probability of gastric cancer development. This paper aims to review the role of H. pylori and its virulence factors in epithelial-mesenchymal transition associated with malignant transformation within the gastric stroma. The reviewed factors included: CagA (cytotoxin-associated gene A) along with induction of cancer stem-cell properties and interaction with YAP (Yes-associated protein pathway), tumor necrosis factor α-inducing protein, Lpp20 lipoprotein, Afadin protein, penicillin-binding protein 1A, microRNA-29a-3p, programmed cell death protein 4, lysosomal-associated protein transmembrane 4β, cancer-associated fibroblasts, heparin-binding epidermal growth factor (HB-EGF), matrix metalloproteinase-7 (MMP-7), and cancer stem cells (CSCs). The review summarizes the most recent findings, providing insight into potential molecular targets and new treatment strategies for gastric cancer

THE STIMULATORY EFFECTS OF TOPICAL APPLICATION OF RADIOACTIVE LANTERN MANTLE POWDER ON WOUND HEALING

Some people in different parts of Iran use burned mantles as a wound healing medicine. To perform surface area measurement, twenty rats were divided randomly into two groups of 10 animals each. The 1st group received topical burned radioactive lantern mantle powder at 1st-3rd day after making excision wounds. The 2nd group received nonradioactive lantern mantle powder. For histological study, 36 male rats randomly divided into two groups of 18 animals each. Full thickness excision wound (314±31.4 mm2) was made on the dorsal neck in all animals after inducing general anesthesia. For the first 3 days, cases received topical application of the radioactive lantern mantle powder. Finally, to measure the tensile strength, an incision was made on the dorsal neck of the rats. Surface area measurement of the wounds showed a progressive surface reduction in both groups. Histological study showed a significant statistically difference between cases and controls with respect to fibrinoid necrosis and neutrophilic exudate at the days 3 and 14. Considering the existence of granulation tissue, a significant difference was observed between case and control groups at days 3 and 7. Tensile strength study showed no significant difference between the cases and controls until 30 days after excision

Electric-field-induced alignment of electrically neutral disk-like particles: modelling and calculation

This work reveals a torque from electric field to electrically neutral flakes that are suspended in a higher electrical conductive matrix. The torque tends to rotate the particles toward an orientation with its long axis parallel to the electric current flow. The alignment enables the anisotropic properties of tiny particles to integrate together and generate desirable macroscale anisotropic properties. The torque was obtained from thermodynamic calculation of electric current free energy at various microstructure configurations. It is significant even when the electrical potential gradient becomes as low as 100 v/m. The changes of electrical, electroplastic and thermal properties during particles alignment were discussed

Lattice Boltzmann Simulation of Solid Particles Motion in a Three Dimensional Flow using Smoothed Profile Method

Three-dimensional particulate flow has been simulated using Lattice Boltzmann Method (LBM). Solid-fluid interaction was modeled based on Smoothed Profile Method (SPM) (Jafari et. al, Lattice-Boltzmann method combined with smoothed-profile method for particulate suspensions, Phys. Rev. E, 2011). In this paper a GPU code based on three-dimensional lattice Boltzmann method and smoothed profile method has been prepared due to the ability of SPM-LBM to perform locally and in parallel mode. Results obtained for sedimentation of one and two spherical particles as well as their behavior in shear flow showed excellent correspondence with previous published works. Computations for a large number of particles sedimentation showed that combination of LBM and SPM on a GPU platform can be considered as an efficient and promising computational frame work in particulate flow simulations

Nano-mechanical properties of Fe-Mn-Al-C lightweight steels

High Al Low-density steels could have a transformative effect on the light-weighting of steel structures for transportation and achieving the desired properties with the minimum amount of Ni is of great interest from an economic perspective. In this study, the mechanical properties of two duplex low-density steels, Fe-15Mn-10Al-0.8C-5Ni and Fe-15Mn-10Al-0.8C (wt.%) were investigated through nano-indentation and simulation through utilization of ab initio formalisms in Density Functional Theory (DFT) in order to establish the hardness resulting from two critical structural features (ߢ-carbides and B2 intermetallic) as a function of annealing temperature (500 − 1050 ℃) and the addition of Ni. In the Ni-free sample, the calculated elastic properties of kappa-carbides were compared with those of the B2 intermetallic Fe3Al − L12, and the role of Mn in the kappa structure and its elastic properties were studied. The Ni-containing samples were found to have a higher hardness due to the B2 phase composition being NiAl rather than FeAl, with Ni-Al bonds reported to be stronger than the Fe-Al bonds. In both samples, at temperatures of 900 ℃ and above, the ferrite phase contained nano-sized discs of B2 phase, wherein the Ni-containing samples exhibited higher hardness, attributed again to the stronger Ni-Al bonds in the B2 phase. At 700 ℃ and below, the nano-sized B2 discs were replaced by micrometre sized needles of kappa in the Ni-free sample resulting in a lowering of the hardness. In the Ni-containing sample, the entire alpha phase was replaced by B2 stringers, which had a lower hardness than the Ni-Al nano-discs due to a lower Ni content in B2 stringer bands formed at 700 ℃ and below. In addition, the hardness of needle-like kappa-carbides formed in alpha phase was found to be a function of Mn content. Although it was impossible to measure the hardness of cuboid kappa particles in gamma phase because of their nano-size, the hardness value of composite phases, e.g. gamma + kappa was measured and reported. All the hardness values were compared and rationalized by bonding energy between different atoms

Ptch2/Gas1 and Ptch1/Boc differentially regulate Hedgehog signalling in murine primordial germ cell migration.

Gas1 and Boc/Cdon act as co-receptors in the vertebrate Hedgehog signalling pathway, but the nature of their interaction with the primary Ptch1/2 receptors remains unclear. Here we demonstrate, using primordial germ cell migration in mouse as a developmental model, that specific hetero-complexes of Ptch2/Gas1 and Ptch1/Boc mediate the process of Smo de-repression with different kinetics, through distinct modes of Hedgehog ligand reception. Moreover, Ptch2-mediated Hedgehog signalling induces the phosphorylation of Creb and Src proteins in parallel to Gli induction, identifying a previously unknown Ptch2-specific signal pathway. We propose that although Ptch1 and Ptch2 functionally overlap in the sequestration of Smo, the spatiotemporal expression of Boc and Gas1 may determine the outcome of Hedgehog signalling through compartmentalisation and modulation of Smo-downstream signalling. Our study identifies the existence of a divergent Hedgehog signal pathway mediated by Ptch2 and provides a mechanism for differential interpretation of Hedgehog signalling in the germ cell niche

The Surgical Infection Society revised guidelines on the management of intra-abdominal infection

Background: Previous evidence-based guidelines on the management of intra-abdominal infection (IAI) were published by the Surgical Infection Society (SIS) in 1992, 2002, and 2010. At the time the most recent guideline was released, the plan was to update the guideline every five years to ensure the timeliness and appropriateness of the recommendations. Methods: Based on the previous guidelines, the task force outlined a number of topics related to the treatment of patients with IAI and then developed key questions on these various topics. All questions were approached using general and specific literature searches, focusing on articles and other information published since 2008. These publications and additional materials published before 2008 were reviewed by the task force as a whole or by individual subgroups as to relevance to individual questions. Recommendations were developed by a process of iterative consensus, with all task force members voting to accept or reject each recommendation. Grading was based on the GRADE (Grades of Recommendation Assessment, Development, and Evaluation) system; the quality of the evidence was graded as high, moderate, or weak, and the strength of the recommendation was graded as strong or weak. Review of the document was performed by members of the SIS who were not on the task force. After responses were made to all critiques, the document was approved as an official guideline of the SIS by the Executive Council. Results: This guideline summarizes the current recommendations developed by the task force on the treatment of patients who have IAI. Evidence-based recommendations have been made regarding risk assessment in individual patients; source control; the timing, selection, and duration of antimicrobial therapy; and suggested approaches to patients who fail initial therapy. Additional recommendations related to the treatment of pediatric patients with IAI have been included. Summary: The current recommendations of the SIS regarding the treatment of patients with IAI are provided in this guideline