PERAN MAHASISWA KKN SEBAGAI TENAGA PENGAJAR UNTUK MENINGKATKAN PENDIDIKAN DI KELURAHAN BRONDONG

Kegiatan pengabdian kepada masyarakat di Kelurahan Brondong, Kecamatan Brondong, Kabupaten Lamongan dikarenakan kurangnya minat belajar anak-anak di masa pendemi covid 19. Mengingat kurangnya kebutuhan pemenuhan belajar. Oleh karena itu tujuan kegiatan ini adalah untuk meningkatkan proses belajar mengajar anak-anak di Kelurahan Brondong serta turut aktif mengikuti kegiatan di Kelurahan Brondong. Metode yang digunakan adalah observasi dan pendampingan. Bentuk kegiatan diantaranya mengajar di MIM 16 Jompang, mengajar mengaji, mengajar Les, Gotong royong,dsb. Mitra dalam kegiatan ini adalah Kelurahan Brondong, Kecamatan Brondong, Hasil dari Program Kuliah Kerja Nyata (KKN) ini dapat meningkatan ilmu dan pengetahuan masyarakat. Mahasiswa mendapat pengalaman dan ilmu kemasyarakatan yang tidak di dapat dibangku kuliah dan masyarakat dapat menyerap ilmu yang dimiliki mahasiswa dalam meningkatkan wawasan mereka untuk kemudian diterapkan dalam kehidapan sehari-hari

Isolation and bioactivity of steroids isolates from petroleum ether fraction of Chlorella sp.

Chlorellasp. is a prospective steroids source which is potentially applied in pharmaceutical field. The aim of this research was to isolate and determine the toxicity and antioxidant activity of steroids compounds in petroleum ether (PE) fraction. Chlorellasp. was cultivated in 4% of sprout extract medium and extracted by maceration using methanol solvent. The methanol extract was partitioned with petroleum ether and the fraction was identified by LC-MS to know the type of steroids compound. The PE fraction contained steroids was separated with preparative thin layer chromatography (Preparative TLC) and column chromatography. The toxicity level and antioxidant assay of steroids isolates was determined by BSLT and DPPH method, respectively. According to phytochemical test and LC-APCI-MS identification, the PE fraction was positive for steroids compounds such as β-sitosterol, stigmasterol, campesterol and erythrodiol. Steroid isolates from TLC and column chromatography separation has toxicity and antioxidant properties. Based on toxicity test, LC50value of steroid isolate by TLC separation was 19.69 ppm, whereas, by column chromatography separation, LC50 value of A10 and A12 isolate was 26.67 and 49.04 ppm, respectively. Antioxidant activity (EC50 value) of steroid isolate by TLC separation was 73.82 ppm. Percentage of antioxidant activity of A2, A8, A10 and A12 isolates (by column chromatography separation) at 50 ppm were 2.60, 4.09, 4.52 and 3.29 %