The Advantage of Foraging Myopically

We study the dynamics of a \emph{myopic} forager that randomly wanders on a lattice in which each site contains one unit of food. Upon encountering a food-containing site, the forager eats all the food at this site with probability $p<1$; otherwise, the food is left undisturbed. When the forager eats, it can wander $\mathcal{S}$ additional steps without food before starving to death. When the forager does not eat, either by not detecting food on a full site or by encountering an empty site, the forager goes hungry and comes one time unit closer to starvation. As the forager wanders, a multiply connected spatial region where food has been consumed---a desert---is created. The forager lifetime depends non-monotonically on its degree of myopia $p$, and at the optimal myopia $p=p^*(\mathcal{S})$, the forager lives much longer than a normal forager that always eats when it encounters food. This optimal lifetime grows as $\mathcal{S}^2/\ln\mathcal{S}$ in one dimension and faster than a power law in $\mathcal{S}$ in two and higher dimensions.Comment: 10 pages, 1o figure

The Advantage of Foraging Myopically

We study the dynamics of a \emph{myopic} forager that randomly wanders on a lattice in which each site contains one unit of food. Upon encountering a food-containing site, the forager eats all the food at this site with probability $p<1$; otherwise, the food is left undisturbed. When the forager eats, it can wander $\mathcal{S}$ additional steps without food before starving to death. When the forager does not eat, either by not detecting food on a full site or by encountering an empty site, the forager goes hungry and comes one time unit closer to starvation. As the forager wanders, a multiply connected spatial region where food has been consumed---a desert---is created. The forager lifetime depends non-monotonically on its degree of myopia $p$, and at the optimal myopia $p=p^*(\mathcal{S})$, the forager lives much longer than a normal forager that always eats when it encounters food. This optimal lifetime grows as $\mathcal{S}^2/\ln\mathcal{S}$ in one dimension and faster than a power law in $\mathcal{S}$ in two and higher dimensions.Comment: 10 pages, 1o figure

High-Throughput Screening Data Interpretation in the Context of In Vivo Transcriptomic Responses to Oral Cr(VI) Exposure

The toxicity of hexavalent chromium [Cr(VI)] in drinking water has been studied extensively, and available in vivo and in vitro studies provide a robust dataset for application of advanced toxicological tools to inform the mode of action (MOA). This study aimed to contribute to the understanding of Cr(VI) MOA by evaluating high-throughput screening (HTS) data and other in vitro data relevant to Cr(VI), and comparing these findings to robust in vivo data, including transcriptomic profiles in target tissues. Evaluation of Tox21 HTS data for Cr(VI) identified 11 active assay endpoints relevant to the Ten Key Characteristics of Carcinogens (TKCCs) that have been proposed by other investigators. Four of these endpoints were related to TP53 (tumor protein 53) activation mapping to genotoxicity (KCC#2), and four were related to cell death/proliferation (KCC#10). HTS results were consistent with other in vitro data from the Comparative Toxicogenomics Database. In vitro responses were compared to in vivo transcriptomic responses in the most sensitive target tissue, the duodenum, of mice exposed to ≤ 180 ppm Cr(VI) for 7 and 90 days. Pathways that were altered both in vitro and in vivo included those relevant to cell death/proliferation. In contrast, pathways relevant to p53/DNA damage were identified in vitro but not in vivo. Benchmark dose modeling and phenotypic anchoring of in vivo transcriptomic responses strengthened the finding that Cr(VI) causes cell stress/injury followed by proliferation in the mouse duodenum at high doses. These findings contribute to the body of evidence supporting a non-mutagenic MOA for Cr(VI)-induced intestinal cancer

Immunologic and Hematopoietic Effects of CD40 Stimulation after Syngeneic Bone Marrow Transplantation in Mice

CD40 is a molecule present on multiple cell types including B lymphocyte lineage cells. CD40 has been shown to play an important role in B cell differentiation and activation in vitro, although little is known concerning the effects of CD40 stimulation in vivo. We therefore examined the effects of CD40 stimulation in mice using a syngeneic bone marrow transplantation (BMT) model in an effort to augment B cell recovery after high dose therapy with hematopoietic reconstitution. After the BMT, mice were treated with or without 2-6 μg of a soluble recombinant murine CD40 ligand (srmCD40L) given intraperitoneally twice a week. A significant increase in B cell progenitors (B220 +/surface IgM -) was observed in the bone marrow of mice receiving the srmCD40L. The treated recipients also demonstrated improved B-cell function with increases in total serum immunoglobulin and increased splenic mitogen responsiveness to LPS being noted. Additionally, srmCD40L treatment promoted secondary lymphoid organ repopulation, accelerating germinal center formation in the lymph nodes. Total B cell numbers in the periphery were not significantly affected even with continuous srmCD40L administration. Lymphocytes obtained from mice treated with the ligand also had increases in T cell mitogen and anti-CD3 mAb responsiveness and acquired the capability to produce IL-4. Surprisingly, treatment with srmCD40L also produced hematopoietic effects in mice, resulting in an increase of BM and splenic hematopoietic progenitor cells in the mice after BMT. Treatment with srmCD40L significantly increased granulocyte and platelet recovery in the peripheral blood. Incubation of BMC with srmCD40L in vitro also resulted in increased progenitor proliferation, demonstrating that the hematopoietic effects of the ligand may be direct. Thus, stimulation of CD40 by its ligand may he beneficial in accelerating both immune and hematopoietic recovery in the setting of bone marrow transplantation

Compassion Stress and the Qualitative Researcher

Human subjects are carefully protected in the research process. However, the same consideration is not currently being given to the qualitative researcher, even those investigating topics that are likely to elicit powerful emotions. The role of researcher’s emotional responses and the self-care strategies that, in some circumstances, are appropriate for the researcher and other research support personnel have not received the attention they deserve in qualitative research literature. Based on experience in conducting research on the topic of self-directed learning and breast cancer, and on the limited literature available, the author makes the case for the use of strategies such as counseling, peer debriefing, and journal writing as means of dealing with the potential for “compassion stress” as experienced by the researcher and other research support personnel. She also suggests that the preparation of social science researchers should include information on appropriate self-care strategies.Yeshttps://us.sagepub.com/en-us/nam/manuscript-submission-guideline

Crescent-Shaped Electron Distributions at the Nonreconnecting Magnetopause: Magnetospheric Multiscale Observations

International audienceCrescent-shaped electron distributions perpendicular to the magnetic field are an important indicator of the electron diffusion region in magnetic reconnection. They can be formed by the electron finite gyroradius effect at plasma boundaries or by demagnetized electron motion. In this study, we present Magnetospheric Multiscale mission observations of electron crescents at the flank magnetopause on 20 September 2017, where reconnection signatures are not observed. These agyrotropic electron distributions are generated by electron gyromotion at the thin electron-scale magnetic boundaries of a magnetic minimum after magnetic curvature scattering. The variation of their angular range in the perpendicular plane is in good agreement with predictions. Upper hybrid waves are observed to accompany the electron crescents at all four Magnetospheric Multiscale spacecraft as a result of the beam-plasma instability associated with these agyrotropic electron distributions. This study suggests electron crescents can be more frequently formed at the magnetopause. Plain Language Summary In this study, we present Magnetospheric Multiscale mission observations of electron crescents at the flank magnetopause and these agyrotropic electron distributions are formed at thin electron-scale magnetic boundaries after electron pitch angle scattering by the curved magnetic field. These results suggest that agyrotropic electron distributions can be more frequently formed at the magnetopause: (1) magnetic reconnection is not necessary, although electron crescents are taken as one of the observational signatures of the electron diffusion region, and (2) agyrotropic electron distributions can cover a large local time range to the flank magnetopause. In addition, upper hybrid waves accompanied with the electron crescents are observed as a result of the beam-plasma interaction associated with these agyrotropic electron distributions. This suggests that high-frequency waves play a role in electron dynamics through wave-particle interactions

Interactions between vaccinia virus and sensitized macrophages in vitro

The action of peritoneal exudate cells (PEC) from normal and vaccinia virus infected mice on infectious vaccinia virus particles was investigatedin vitro. PEC from immune mice showed a significantly higher infectivity titre reduction (virus clearance, VC) than normal cells. This effect could be clearly attributed to the macrophage. Vaccinia virus multiplied in PEC from normal animals while there was no virus propagation in cells from immunized mice. The release of adsorbed or engulfed virus was reduced significantly in PEC from immunized animals. Anti-vaccinia-antibodies seem to activate normal macrophages to increased virus clearance. This stimulating effect was demonstrable only in the IgG fraction of the antiserum. The activity of macrophages from mice injected three times over a period of 14 days with vaccinia virus could be entirely blocked with anti-mouse-IgG, while PEC from mice injected one time six days previously were not inhibited