Antioxidant capacity and total phenols richness of Cistanche violacea hosting Zygophyllum album

Cistanche violacea is an Orobanchaceae holoparsite hosting chenopodiaceous, including Zygophyllum album. While Cistanche violacea, is known to have edible uses in the folk medicine, no data have been reported before. In the present study, chemical assays were undertaken in order to emphasize the Cistanche violacea antioxidant capacity and total phenols richness. Our data showed higher phenolic contents were measured in Cistanche flowers and Zygophyllum leaves, however Cistanche bulb was the most enriched in flavonoïds. Antioxidant activities were evaluated in methanolic extract of Cistanche violacea flowers and bulb among the host Zygophyllum album leaves. Cistanche flowers and Zygophyllum leaves exhibited the strongest scavenging 2,2-diphenyl-2-picrylhydrazyl radical (DPPH) activity. The metal chelating activity was the highest in Cistanche bulb and Zygophyllum leaves compared to Cistanche flowers. Conversely, the ferric reducing power was significantly higher in Cistanche flowers relative to its bulb and Zygophyllum leaves

Peptídeo do hidrolisado protéico de RuBisCO e sua aplicação na preservação de carne bovina

The objective of this work was to purify the small (705 Da) and hydrophilic antimicrobial Arg-Asp-Arg-Phe-Leu peptide from RuBisCO protein hydrolysate and to evaluate its effect on the microbiological and oxidative stability of beef mince during refrigeration. RuBisCO was obtained from alfafa green juice. The peptide extract was fractionated using RP-HPLC, and the active fractions were analyzed by liquid chromatography, electrospray ionization, and tandem mass spectrometry (LC-ESI-MS). Beef mince was analyzed in the following treatments: negative control, meat with two different BHT concentrations of 0.1 and 0.5% (w/w), and meat with two different Arg-Asp-Arg-Phe-Leu peptide concentrations of 0.1 and 0.5% (w/w). Lipid oxidation using the thio-barbituric acid-reactive substance (TBARS) values were significantly affected by the storage period and the concentration of bioactive peptide. Arg-Asp-Arg-Phe-Leu, a small antibacterial peptide from RuBisCO, can be isolated and purified by HPLC from alfafa green juice with retention time between 10 and 50 min, which corresponds to antimicrobial peptides. RuBisCO peptide Arg-Asp-Arg-Phe-Leu 0.5% increases oxidative stability of beef mince during refrigeration. RuBisCO peptide Arg-Asp-Arg-Phe-Leu inhibit microbial growth under refrigeration for 11 days.O objetivo deste trabalho foi purificar peptídeo antimicrobiano pequeno (705 Da) e hidrofílico Arg-Asp-Arg-Phe-Leu a partir do hidrolisado proteico RuBisCO e avaliar seu efeito na estabilidade microbiológica e oxidativa de carne bovina moída durante refrigeração. RuBisCO foi obtida a partir do suco verde de alfafa. O extrato peptídico foi fracionado utilizando RP-HPLC, e as frações ativas foram analisadas por cromatografia líquida, ionização por eletrospray e espectrometria de massa em tandem (LC-ESI-MS). A carne bovina moída foi analisada nos seguintes tratamentos: controle negativo, carne com duas concentrações diferentes de BHT de 0,1 e 0,5% (p/p) e duas concentrações de peptideo Arg-Asp-Arg-Phe-Leu de 0,1 e 0,5% (p/p). Os valores de oxidação lipídica utilizando a substância reativa ao ácido tiobarbitúrico (TBARS) foram significativamente afetados pelo período de armazenamento e pela concentração do peptídeo bioativo. Arg-Asp-Arg-Phe-Leu, um pequeno peptídeo de RuBisCO, pode ser isolado e purificado por HPLC de suco verde de alfafa com tempos de retenção de 10 e 50 min, o que corresponde ao peptídeo antimicrobiano. O peptídeo Arg-Asp-Arg-Phe-Leu 0,5% aumenta a estabilidade de oxidação da carne moída durante a refrigeração. O peptídeo Arg-Asp-Arg-Phe-Leu de RuBisCO inibe o crescimento microbiano sob refrigeração durante 11 dias

Peptide from RuBisCO protein hydrolysate and its application in beef meat preservation

Abstract The objective of this work was to purify the small (705 Da) and hydrophilic antimicrobial Arg-Asp-Arg-Phe-Leu peptide from RuBisCO protein hydrolysate and to evaluate its effect on the microbiological and oxidative stability of beef mince during refrigeration. RuBisCO was obtained from alfafa green juice. The peptide extract was fractionated using RP-HPLC, and the active fractions were analyzed by liquid chromatography, electrospray ionization, and tandem mass spectrometry (LC-ESI-MS). Beef mince was analyzed in the following treatments: negative control, meat with two different BHT concentrations of 0.1 and 0.5% (w/w), and meat with two different Arg-Asp-Arg-Phe-Leu peptide concentrations of 0.1 and 0.5% (w/w). Lipid oxidation using the thio-barbituric acid-reactive substance (TBARS) values were significantly affected by the storage period and the concentration of bioactive peptide. Arg-Asp-Arg-Phe-Leu, a small antibacterial peptide from RuBisCO, can be isolated and purified by HPLC from alfafa green juice with retention time between 10 and 50 min, which corresponds to antimicrobial peptides. RuBisCO peptide Arg-Asp-Arg-Phe-Leu 0.5% increases oxidative stability of beef mince during refrigeration. RuBisCO peptide Arg-Asp-Arg-Phe-Leu inhibit microbial growth under refrigeration for 11 days

Value added products from cuttlefish (Sepia officinalis) for food and nutraceutical applications

Le travail de la thèse s’inscrit dans le cadre de la valorisation enzymatique des produits et des co-produits de la pêche et ce dans l’optique de produire des fonctions et des produits de haute valeur ajoutée. Dans un premier temps, on s’est intéressé à la purification et la caractérisation biochimiques des protéases digestives (trypsine, chymotrypsine et cathepsine D) à partir de l’hépatopancréas de la seiche commune (S. officinalis). Ensuite, nous avons utilisé ces protéases digestives ainsi que d’autres protéases d’origine microbienne dans l’élaboration des hydrolysats protéiques enrichis en peptides bioactifs. 23 nouveaux peptides antihypertensifs ont été identifiés. Également, l’effet antihypertenseur in vivo de certains peptides a été prouvé. Dans un autre volet, on s’est intéressé à l’étude de l’influence de degré d’hydrolyse et le type d’enzyme sur les propriétés fonctionnelles et biologiques dans les hydrolysats protéiques préparés à partir des co-produits (peau et viscères) et le muscle de la seiche commune. Enfin, un procédé enzymatique destiné à l’extraction de la gélatine à partir de la peau de la seiche commune a été développé. L’étude comparative de certaines propriétés fonctionnelles et texturales de la gélatine extraite avec celle d’origine bovine commerciale a été aussi réalisée.The aim of this project was to improve the biotechnological exploitation of marine ressources to obtain value added products for food and nutraceutical applications. First, several digestives proteases (trypsin, chymotrypsin and cathepsin D) from the hepatopancreas of the cuttlefish (Sepia officinalis) were purified and characterized. In the second part, these digestive proteases and other microbial proteases were used to generate protein hydrolysates enriched in bioactive peptides. 23 novel ACE inhibitory peptides were isolated and identified. In addition, the antihypertensive effect in vivo of some peptides was confirmed. The influence of the degree of hydrolysis and enzyme type on functional and bioactive properties of protein hydrolysates from cuttlefish by-products (skin and viscera) and muscle of the same species was studied. Finally, gelatin from the skin of cuttlefish was extracted using protease-aided process. The characteristics, functional and textural properties of this protein were investigated and compared to those ofcommercial bovine gelatin

Valorisation d’effluents de brasserie dans la production de microalgues pour l’alimentation animale : Focus sur le projet AQUILOS

International audienc

Les microorganismes comme potentielles sources de composés antioxydants

International audienc

Exploration de l’ultrafiltration tangentielle pour le fractionnement et la récupération des produits bio-fonctionnelles issus des macroalgues

International audienc

Development of Bioprocesses for Potential Functional Ingredients from Marine products

Editorial.- 1 páginaPeer reviewe

Investissement dans la R&D et développement d'une stratégie territoriale autour d’un site agro-industriel de référence : le cas de la bioraffinerie de Bazancourt-Pomacle

International audienceLa faim, le changement climatique et la pénurie d'énergie sont des défis simultanés auxquels nous sommes confrontés. La bioéconomie semble être une solution potentielle et globale. Basée sur les bioraffineries, elle n'est pas qu'un concept théorique comme peut le démontrer le site agro-industriel de Bazancourt-Pomacle. Cette bioraffinerie est l'une des plus connues en Europe et a récemment été labellisée « territoire d'industrie ». Située près de Reims (51 – France), elle est reconnue comme une bioraffinerie intégrée, c’est-à-dire un modèle d'écologie industrielle et de bioéconomie rurale, fabriquant des ingrédients pour l'alimentation humaine et animale, des carburants et des produits chimiques. Elle met en évidence le potentiel de la bioéconomie industrielle pour remplacer les combustibles fossiles (charbon, pétrole et gaz naturel) par des produits plus durables et renouvelables (principalement les agro-ressources et la biomasse). Ce site repose sur une combinaison d'installations industrielles variées, un développement basé sur l'innovation et un fonctionnement fondé sur la connaissance. Il est le résultat de 70 ans d'initiatives continues. Cette intervention présentera, à travers plusieurs exemples, le rôle majeur de la recherche et son approche interdisciplinaire nécessaire à la valorisation des biomasses dans ce développement, soutenant ainsi la capacité d’adaptation d'un secteur dans un environnement évolutif en se réinventant pour surmonter ses faiblesses grâce à l'innovation

Biopeptides derived from red macroalgae protein and their potential as heart health-promoting functional food ingredients

International audienceAim: Currently, red seaweed has been demonstrated as an important economic biomass with high contents and quality of protein and essential amino acids, potentially making it an attractive and promising alternative protein source for global food sustainability. Angiotensin-converting enzyme (ACE) has a significant role in the regulation of blood pressure and ACE inhibition with inhibitory peptides is considered as a major target to prevent hypertension. Until now, very few studies on ACE inhibitory peptides from red macroalgae have been conducted. Thus, we investigated the generation, isolation and characterization of ACE inhibitory peptides from Gracilaria gracilis proteins by enzymatic hydrolysis for use as a potential functional food ingredients.Method: Firstly, the red seaweed proteins were extracted using different cell disruption strategies (chemical, physical and enzymatic treatments) applied individually or in combination. The protein content of the raw material and the resulting extracts were determined and amino acid profiling was carried out using an amino acid analyzer. Secondly, G. gracilis proteins were hydrolyzed using various food grade proteases to generate bioactive peptides, and their ACE inhibitory activities were assessed. The G. gracilis protein hydrolysates (GGPHs) were further fractionated and purified by membrane ultrafiltration and reverse-phase high performance liquid chromatography. Fractions and peptides were analysed by mass spectrometry. Furthermore, antihypertensive effects of peptides in spontaneously hypertensive rats (SHRs) were tested.Results: Among the different methods of extraction investigated, it was found that the protein extraction efficiency was significantly increased by the use of cocktail based to proteases and polysaccharide-degrading enzymes compared to when enzyme was used alone. In terms of protein hydrolysis, the alcalase-digested hydrolysates exhibited the highest ACE inhibitory activity. Moreover, the degree of hydrolysis and anti-ACE potential of GGPHs can vary depending on the proteases used. After fractionation and purification by ultrafiltration, the <1 kDa fraction was the most active as inhibitor of the in vitro activities of ACE. Some of the peptides identified revealed strong blood pressure reductions in SHRs.Conclusion: The biopeptides derived from protein hydrolysates of G. gracilis would be a heart health-promoting functional ingredients for nutraceuticals acting against hypertension and its related diseases