Visual Electrodiagnostic Testing in Birdshot Chorioretinopathy

Birdshot chorioretinopathy (BSCR) is a rare form of autoimmune posterior uveitis that can affect the visual function and, if left untreated, can lead to sight-threatening complications and loss of central vision. We performed a systematic search of the literature focused on visual electrophysiology studies, including electroretinography (ERG), electrooculography (EOG), and visual evoked potentials (VEP), used to monitor the progression of BSCR and estimate treatment efficacy. Many reports were identified, including using a variety of methodologies and patient populations, which makes a direct comparison of the results difficult, especially with some of the earlier studies using nonstandardized methodology. Several different electrophysiological parameters, like EOG Arden’s ratio and the multifocal ERG response densities, are reported to be widely affected. However, informal consensus emerged in the past decade that the full-field ERG light-adapted 30 Hz flicker peak time is one of the most sensitive electrophysiological parameters. As such, it has been used widely in clinical trials to evaluate drug safety and efficacy and to guide therapeutic decisions in clinical practice. Despite its wide use, a well-designed longitudinal multicenter study to systematically evaluate and compare different electrophysiological methods or parameters in BSCR is still lacking but would benefit both diagnostic and therapeutic decisions

CRAI Biblioteca del Campus de Mundet. Memòria d'activitats 2016

Memòria que recull les activitats realitzades al CRAI Biblioteca del Campus de Mundet durant l'any 2016

Inhibition or Stimulation of Autophagy Affects Early Formation of Lipofuscin-Like Autofluorescence in the Retinal Pigment Epithelium Cell

The accumulation of lipofuscin in the retinal pigment epithelium (RPE) is dependent on the effectiveness of photoreceptor outer segment material degradation. This study explored the role of autophagy in the fate of RPE lipofuscin degradation. After seven days of feeding with either native or modified rod outer segments, ARPE-19 cells were treated with enhancers or inhibitors of autophagy and the autofluorescence was detected by fluorescence-activated cell sorting. Supplementation with different types of rod outer segments increased lipofuscin-like autofluorescence (LLAF) after the inhibition of autophagy, while the induction of autophagy (e.g., application of rapamycin) decreased LLAF. The effects of autophagy induction were further confirmed by Western blotting, which showed the conversion of LC3-I to LC3-II, and by immunofluorescence microscopy, which detected the lysosomal activity of the autophagy inducers. We also monitored LLAF after the application of several autophagy inhibitors by RNA-interference and confocal microscopy. The results showed that, in general, the inhibition of the autophagy-related proteins resulted in an increase in LLAF when cells were fed with rod outer segments, which further confirms the effect of autophagy in the fate of RPE lipofuscin degradation. These results emphasize the complex role of autophagy in modulating RPE autofluorescence and confirm the possibility of the pharmacological clearance of RPE lipofuscin by small molecules

Relationship between optical coherence tomography sector peripapillary angioflow-density and Octopus visual field cluster mean defect values

PURPOSE: To compare the relationship of Octopus perimeter cluster mean-defect (cluster MD) values with the spatially corresponding optical coherence tomography (OCT) sector peripapillary angioflow vessel-density (PAFD) and sector retinal nerve fiber layer thickness (RNFLT) values. METHODS: High quality PAFD and RNFLT images acquired on the same day with the Angiovue/RTVue-XR Avanti OCT (Optovue Inc., Fremont, USA) on 1 eye of 27 stable early-to-moderate glaucoma, 22 medically controlled ocular hypertensive and 13 healthy participants were analyzed. Octopus G2 normal visual field test was made within 3 months from the imaging. RESULTS: Total peripapillary PAFD and RNFLT showed similar strong positive correlation with global mean sensitivity (r-values: 0.6710 and 0.6088, P<0.0001), and similar (P = 0.9614) strong negative correlation (r-values: -0.4462 and -0.4412, P</=0.004) with global MD. Both inferotemporal and superotemporal sector PAFD were significantly (</=0.039) lower in glaucoma than in the other groups. No significant difference between the corresponding inferotemporal and superotemporal parameters was seen. The coefficient of determination (R2) calculated for the relationship between inferotemporal sector PAFD and superotemporal cluster MD (0.5141, P<0.0001) was significantly greater than that between inferotemporal sector RNFLT and superotemporal cluster MD (0.2546, P = 0.0001). The R2 values calculated for the relationships between superotemporal sector PAFD and RNFLT, and inferotemporal cluster MD were similar (0.3747 and 0.4037, respectively, P<0.0001). CONCLUSION: In the current population the relationship between inferotemporal sector PAFD and superotemporal cluster MD was strong. It was stronger than that between inferotemporal sector RNFLT and superotemporal cluster MD. Further investigations are necessary to clarify if our results are valid for other populations and can be usefully applied for glaucoma research

Visual Electrodiagnostic Testing in Birdshot Chorioretinopathy

Birdshot chorioretinopathy (BSCR) is a rare form of autoimmune posterior uveitis that can affect the visual function and, if left untreated, can lead to sight-threatening complications and loss of central vision. We performed a systematic search of the literature focused on visual electrophysiology studies, including electroretinography (ERG), electrooculography (EOG), and visual evoked potentials (VEP), used to monitor the progression of BSCR and estimate treatment efficacy. Many reports were identified, including using a variety of methodologies and patient populations, which makes a direct comparison of the results difficult, especially with some of the earlier studies using nonstandardized methodology. Several different electrophysiological parameters, like EOG Arden’s ratio and the multifocal ERG response densities, are reported to be widely affected. However, informal consensus emerged in the past decade that the full-field ERG light-adapted 30 Hz flicker peak time is one of the most sensitive electrophysiological parameters. As such, it has been used widely in clinical trials to evaluate drug safety and efficacy and to guide therapeutic decisions in clinical practice. Despite its wide use, a well-designed longitudinal multicenter study to systematically evaluate and compare different electrophysiological methods or parameters in BSCR is still lacking but would benefit both diagnostic and therapeutic decisions

Natural Protection of Ocular Surface from Viral Infections – A Hypothesis

A pandemic outbreak of a viral respiratory infection (COVID-19) caused by a coronavirus (SARS-CoV-2) prompted a multitude of research focused on various aspects of this disease. One of the interesting aspects of the clinical manifestation of the infection is an accompanying ocular surface viral infection, viral conjunctivitis. Although occasional reports of viral conjunctivitis caused by this and the related SARS-CoV virus (causing the SARS outbreak in the early 2000s) are available, the prevalence of this complication among infected people appears low (~1%). This is surprising, considering the recent discovery of the presence of viral receptors (ACE2 and TMPRSS2) in ocular surface tissue. The discrepancy between the theoretically expected high rate of concurrence of viral ocular surface inflammation and the observed relatively low occurrence can be explained by several factors. In this work, we discuss the significance of natural protective factors related to anatomical and physiological properties of the eyes and preventing the deposition of large number of virus-loaded particles on the ocular surface. Specifically, we advance the hypothesis that the standing potential of the eye plays an important role in repelling aerosol particles (microdroplets) from the surface of the eye and discuss factors associated with this hypothesis, possible ways to test it and its implications in terms of prevention of ocular infections

Senescence of human multifocal electroretinogram components: a localized approach.

BackgroundPrevious studies have shown significant age-related changes in the first-order kernel of multifocal ERG (mfERG) responses. All of these reports were based upon ring averages across the retinal field. This study was carried out to determine age-related changes in the localized response and localized variability in the mfERG parameters: N1P1 amplitude, scalar product and implicit time of P1.MethodsMfERG recordings from 70 normal phakic subjects (ages 9-80 years) were analyzed with VERIS 4.8. Scalar product values (for each hexagon based on ring average templates) were obtained and analyzed for age-related changes. Statistical measures such as coefficient of variation (CV) and parameters of a linear regression model were applied. Point-by-point comparisons were made across hemifields.ResultsEach localized response showed a significant aging effect either in scalar product or in N1P1 amplitude. The average decline of the response was approximately 5% per decade, varying from 3.3% (peripherally) to 7.5% (perifoveally). The decline was significantly higher for the superior than for the inferior retina for amplitude parameters, corresponding to larger increases in P1 implicit time. The relative rate of change with age was similar for the nasal and the temporal retina. The average CV for all subjects at all locations was 29.4% (+/-4.1%).ConclusionsThe localized approach revealed patterns of age-related change that were not apparent in the ring averages. Information about changes in discrete retinal areas with age should make the mfERG more useful in quantitatively monitoring progression of retinal disease

Senescence of human multifocal electroretinogram components: a localized approach.

BackgroundPrevious studies have shown significant age-related changes in the first-order kernel of multifocal ERG (mfERG) responses. All of these reports were based upon ring averages across the retinal field. This study was carried out to determine age-related changes in the localized response and localized variability in the mfERG parameters: N1P1 amplitude, scalar product and implicit time of P1.MethodsMfERG recordings from 70 normal phakic subjects (ages 9-80 years) were analyzed with VERIS 4.8. Scalar product values (for each hexagon based on ring average templates) were obtained and analyzed for age-related changes. Statistical measures such as coefficient of variation (CV) and parameters of a linear regression model were applied. Point-by-point comparisons were made across hemifields.ResultsEach localized response showed a significant aging effect either in scalar product or in N1P1 amplitude. The average decline of the response was approximately 5% per decade, varying from 3.3% (peripherally) to 7.5% (perifoveally). The decline was significantly higher for the superior than for the inferior retina for amplitude parameters, corresponding to larger increases in P1 implicit time. The relative rate of change with age was similar for the nasal and the temporal retina. The average CV for all subjects at all locations was 29.4% (+/-4.1%).ConclusionsThe localized approach revealed patterns of age-related change that were not apparent in the ring averages. Information about changes in discrete retinal areas with age should make the mfERG more useful in quantitatively monitoring progression of retinal disease