15 research outputs found

    Risk as a process: a history informed hazard planning approach applied to the 2018 post-fire debris flows, Montecito, California

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    Historical information about floods is not commonly used in the US to inform land use planning decisions. Rather, the current approach to managing floods is based on static maps derived from computer simulations of the area inundated by floods of specified return intervals. These maps provide some information about flood hazard, but they do not reflect the underlying processes involved in creating a flood disaster, which typically include increased exposure due to building on flood-prone land, nor do they account for the greater hazard resulting from wildfire. We developed and applied an approach to analyze how exposure has evolved in flood hazard zones in Montecito, California, an area devastated by post-fire debris flows in January 2018. By combining historical flood records of the past 200 years, human development records of the past 100 years, and geomorphological understanding of debris flow generation processes, this approach allows us to look at risk as a dynamic process influenced by physical and human factors, instead of a static map. Results show that floods after fires, in particular debris flows and debris laden floods, are very common in Montecito (15 events in the last 200 years), and that despite policies discouraging developments in hazard areas, developments in hazard zones have increased substantially since Montecito joined the National Flood Insurance Program in 1979. We also highlight the limitation of using conventional Flood Insurance Rate Maps (FIRMs) to manage land use in alluvial fan areas such as Montecito. The knowledge produced in this project can help Montecito residents better understand how they came to be vulnerable to floods and identify action they are taking now that might increase or reduce their vulnerability to the next big flood. This science-history-centric approach to understand hazard and exposure evolution using geographic information systems (GIS) and historical records, is generalizable to other communities seeking to better understand the nature of the hazard they are exposed to and some of the root causes of their vulnerabilities, in other words, both the natural and social processes producing disasters

    Risk as a process: a history informed hazard planning approach applied to the 2018 post-fire debris flows, Montecito, California

    Get PDF
    International audienceHistorical information about floods is not commonly used in the US to inform land use planning decisions. Rather, the current approach to managing floods is based on static maps derived from computer simulations of the area inundated by floods of specified return intervals. These maps provide some information about flood hazard, but they do not reflect the underlying processes involved in creating a flood disaster, which typically include increased exposure due to building on flood-prone land, nor do they account for the greater hazard resulting from wildfire. We developed and applied an approach to analyze how exposure has evolved in flood hazard zones in Montecito, California, an area devastated by post-fire debris flows in January 2018. By combining historical flood records of the past 200 years, human development records of the past 100 years, and geomorphological understanding of debris flow generation processes, this approach allows us to look at risk as a dynamic process influenced by physical and human factors, instead of a static map. Results show that floods after fires, in particular debris flows and debris laden floods, are very common in Montecito (15 events in the last 200 years), and that despite policies discouraging developments in hazard areas, developments in hazard zones have increased substantially since Montecito joined the National Flood Insurance Program in 1979. We also highlight the limitation of using conventional Flood Insurance Rate Maps (FIRMs) to manage land use in alluvial fan areas such as Montecito. The knowledge produced in this project can help Montecito residents better understand how they came to be vulnerable to floods and identify action they are taking now that might increase or reduce their vulnerability to the next big flood. This science-history-centric approach to understand hazard and exposure evolution using geographic information systems (GIS) and historical records, is generalizable to other communities seeking to better understand the nature of the hazard they are exposed to and some of the root causes of their vulnerabilities, in other words, both the natural and social processes producing disasters

    RepB C-terminus mutation of a pRi-repABC binary vector affects plasmid copy number in Agrobacterium and transgene copy number in plants.

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    A native repABC replication origin from pRiA4b was previously reported as a single copy plasmid in Agrobacterium tumefaciens and can improve the production of transgenic plants with a single copy insertion of transgenes when it is used in binary vectors for Agrobacterium-mediated transformation. A high copy pRi-repABC variant plasmid, pTF::Ri, which does not improve the frequency of single copy transgenic plants, has been reported in the literature. Sequencing the high copy pTF::Ri repABC operon revealed the presence of two mutations: one silent mutation and one missense mutation that changes a tyrosine to a histidine (Y299H) in a highly conserved area of the C-terminus of the RepB protein (RepBY299H). Reproducing these mutations in the wild-type pRi-repABC binary vector showed that Agrobacterium cells with the RepBY299H mutation grow faster on both solidified and in liquid medium, and have higher plasmid copy number as determined by ddPCR. In order to investigate the impact of the RepBY299H mutation on transformation and quality plant production, the RepBY299H mutated pRi-repABC binary vector was compared with the original wild-type pRi-repABC binary vector and a multi-copy oriV binary vector in canola transformation. Molecular analyses of the canola transgenic plants demonstrated that the multi-copy pRi-repABC with the RepBY299H mutation provides no advantage in generating high frequency single copy, backbone-free transgenic plants in comparison with the single copy wild-type pRi-repABC binary vector
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