27 research outputs found

    Performance Evaluation of AODV, DSR, DYMO & ZRP in Cost 231 Walfisch-Ikegami Path Loss Propagation Model

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    A Mobile Ad hoc NETwork is a kind of wireless ad-hoc network, and is a self configuring network of mobile routers connected by wireless links. Mobile Ad-Hoc Network (MANET) is a wireless network without infrastructure. Self configurability and easy deployment feature of the MANET resulted in numerous applications in this modern era. Efficient routing protocols will make MANETs reliable. Various research communities are working in field of MANET and trying to adopt the protocols and technology in other applications as well. In this work, we present investigations on the behavior of various routing protocol of MANET with a Cost 231 Walfisch-Ikegami Propagation Model. We evaluate the performance of four different ad-hoc routing protocols on four performance metrics such as Average Jitter, Average End-to-End Delay, Throughput, and Packet Delivery Fraction with varying Pause Time. From the simulation results it is concluded that DSR is better in transmission of packets per unit time and maximum number of packets reached their destination successfully with some delays, i.e. PDF & Throughput is more and Average jitter & end-to-end delay is less. Whereas AODV & ZRP having almost same values in all of the performance metrics, they transmit packets with very less delay but transmits less packets to their destination as compare to DSR.

    Dehydratase mediated 1-propanol production in metabolically engineered Escherichia coli

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    Abstract Background With the increasing consumption of fossil fuels, the question of meeting the global energy demand is of great importance in the near future. As an effective solution, production of higher alcohols from renewable sources by microorganisms has been proposed to address both energy crisis and environmental concerns. Higher alcohols contain more than two carbon atoms and have better physiochemical properties than ethanol as fuel substitutes. Results We designed a novel 1-propanol metabolic pathway by expanding the well-known 1,2-propanediol pathway with two more enzymatic steps catalyzed by a 1,2-propanediol dehydratase and an alcohol dehydrogenase. In order to engineer the pathway into E. coli, we evaluated the activities of eight different methylglyoxal synthases which play crucial roles in shunting carbon flux from glycolysis towards 1-propanol biosynthesis, as well as two secondary alcohol dehydrogenases of different origins that reduce both methylglyoxal and hydroxyacetone. It is evident from our results that the most active enzymes are the methylglyoxal synthase from Bacillus subtilis and the secondary alcohol dehydrogenase from Klebsiella pneumoniae, encoded by mgsA and budC respectively. With the expression of these two genes and the E. coli ydjG encoding methylglyoxal reductase, we achieved the production of 1,2-propanediol at 0.8 g/L in shake flask experiments. We then characterized the catalytic efficiency of three different diol dehydratases on 1,2-propanediol and identified the optimal one as the 1,2-propanediol dehydratase from Klebsiella oxytoca, encoded by the operon ppdABC. Co-expressing this enzyme with the above 1,2-propanediol pathway in wild type E. coli resulted in the production of 1-propanol at a titer of 0.25 g/L. Conclusions We have successfully established a new pathway for 1-propanol production by shunting the carbon flux from glycolysis. To our knowledge, it is the first time that this pathway has been utilized to produce 1-propanol in E. coli. The work presented here forms a basis for further improvement in production. We speculate that dragging more carbon flux towards methylglyoxal by manipulating glycolytic pathway and eliminating competing pathways such as lactate generation can further enhance the production of 1-propanol.</p

    ENHANCING CIRCULAR MICROSTRIP PATCH ANTENNA PERFORMANCE USING MACHINE LEARNING MODELS

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    Machine learning (ML) will be heavily used in the future generation of wireless communication networks. The development of diverse communication-based applications is expected to boost coverage and spectrum efficiency in relation to conventional systems. ML may be employed to develop solutions in a wide range of domains, such as antennas. This article describes the design and optimization of a circular patch antenna. The optimization is done through ML algorithms. Six ML models, Decision Tree, Random Forest, XG-Boost Regression, K-Nearest Neighbour (KNN), Gradient Boosting Regression (GBR), and Light Gradient Boosting Regression (LGBR), were employed in this work to predict the antenna's return loss (S11). The findings show that all of these models work well, with KNN having the highest accuracy in predicting return loss of 98.5%. The antenna design & optimization process can be accelerated with the support of ML. These developments allow designers to push beyond the limits of antenna technology, optimize performance, and offer novel solutions for emerging applications such as 5G, 6G, IoT, and flexible wireless communication systems)

    Single feature polymorphisms (SFPs) for drought tolerance in pigeonpea (Cajanus spp.)

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    Single feature polymorphisms (SFPs) are microarray-based molecular markers that are detected by hybridization of DNA or cRNA to oligonucleotide probes. With an objective to identify the potential polymorphic markers for drought tolerance in pigeonpea [Cajanus cajan (L.) Millspaugh], an important legume crop for the semi-arid tropics but deficient in genomic resources, Affymetrix Genome Arrays of soybean (Glycine max), a closely related species of pigeonpea were used on cRNA of six parental genotypes of three mapping populations of pigeonpea segregating for agronomic traits like drought tolerance and pod borer (Helicoverpa armigiera) resistance. By using robustified projection pursuit method on 15 pair-wise comparisons for the six parental genotypes, 5,692 SFPs were identified. Number of SFPs varied from 780 (ICPL 8755 × ICPL 227) to 854 (ICPL 151 × ICPL 87) per parental combination of the mapping populations. Randomly selected 179 SFPs were used for validation by Sanger sequencing and good quality sequence data were obtained for 99 genes of which 75 genes showed sequence polymorphisms. While associating the sequence polymorphisms with SFPs detected, true positives were observed for 52.6% SFPs detected. In terms of parental combinations of the mapping populations, occurrence of true positives was 34.48% for ICPL 151 × ICPL 87, 41.86% for ICPL 8755 × ICPL 227, and 81.58% for ICP 28 × ICPW 94. In addition, a set of 139 candidate genes that may be associated with drought tolerance has been identified based on gene ontology analysis of the homologous pigeonpea genes to the soybean genes that detected SFPs between the parents of the mapping populations segregating for drought tolerance

    Retrieval of a metallic obstruction from the root canal of a premolar using Masserann technique

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    A metallic obstruction in the root canal blocks canal cleaning and shaping procedures and requires either bypassing or retrieval. Many methods have been recommended to retrieve a metallic obstruction from the root canal. This article describes the retrieval of a metallic obstruction from the root canal of a premolar using Masserann technique to facilitate endodontic retreatment. Masserann technique is said to have limited application in posteriors. However, in this case, the obstruction was successfully retrieved by employing Masserann technique which consisted of using a trephan to cut the dentine and extractor tube to retrieve the obstruction. The retrieved obstruction was found to be a separated H-file. Endodontic retreatment was completed following the detection and negotiation of an extra canal in the same tooth

    Power Quality Diagnosis Due to Integration of Renewable Energy Source at Utility Grid

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    Article outlines hybrid energy sources for fulfil supply demand through proposed approach. The hybrid renewable energy sources generation capacity of 6 kW as solar power and 50 kW power as solid oxide fuel cell plant with storage system are interfaced to the DC bus in proposed paper for performance diagnosis on the basis of power demand as per utility load application by conversion of DC power to AC power and power quality of grid/load parameters collected on PCC, which affected by RE source integration at utility bus in terms of power quality parameters as per event ON/OFF grid mode. For this study design a two-bus test system, first bus is DC bus for integration of solar PV plant and solid oxide fuel cell power plant to generate hybrid DC power which convert to AC power by using inverter technology and deliver to the second bus of utility load bus in proposed test system for application of utility load as well as utility grid as per event proposed. A low pass LC filter is modeled to remove high frequency harmonics from the inverter output which is fed to grid as well as test system of utility network

    Detection and endodontic management of radix entomolaris: Report of case series

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    Radix entomolaris (RE) is considered to be an Asiatic trait. However, its prevalence in Indian population is found to be lower than in other Asian races. Nevertheless, its awareness and identification is vital to achieve endodontic success. This article presents a review on clinical approach and a case series on the detection and endodontic management of RE in mandibular first molars. Radix entomolaris was detected by identifying the presence of a double or extra root outline in the preoperative radiograph, modifying the access opening and closely inspecting the pulp chamber and was endodontically treated following cleaning, shaping, and obturation of the canals. Achieving the endodontic success in the presence of an RE requires knowledge about its prevalence, diagnosis, morphology, canal configuration, and clinical approach
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