The Appalachian Rollergirls: A Documentary

In the mountainous town of Boone, North Carolina, the Appalachian Rollergirls spend two days per week practicing and perfecting their sport. Their extreme dedication symbolizes the true spirit of roller derby—to persevere in the face of geographical difficulties, varying identities, and an area known for a 2007 football game upset against Michigan. This documentary will show how the Appalachian Rollergirls practice, prepare for bouts (games), and strive to be the very best. This “slice of life” perspective will show how extreme does not always have a negative connotation; indeed, in the sport of roller derby in Appalachia, extreme is the highest compliment one could receive

Consensus recommendations for trabecular meshwork cell isolation, characterization and culture

Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the standard procedures used for the isolation of TM cells from several animal species including humans, and the methods used to validate their identity. Having a set of standard practices for TM cells will increase the scientific rigor when used as a model, and enable other researchers to replicate and build upon previous findings