Plainview-Old Bethpage and Long Island Historical Bibliography

2 p. ; 28 cm.https://commons.library.stonybrook.edu/government-documents-regional-digital-research-collection/1150/thumbnail.jp

Mathematical Modeling of a Bioluminescent E. Coli Based Biosensor

In this work we present a mathematical model for the bioreporter activity of an E. coli based bioluminescent bioreporter. This bioreporter is based on a genetically modified E. coli which harbors the recA promoter, a member of the bacterial SOS response, fused to the bacterial luminescence (lux) genes. This bioreporter responds to the presence of DNA damaging agents such as heavy metals, H2O2 and Nalidixic Acid (NA) that activate the SOS response. In our mathematical model we implemented basic physiological mechanisms such as: the penetration of the NA into the biosensor; gyrase enzyme inhibition by the NA; gyrase level regulation; creation of chromosomal DNA damage; DNA repair and release of ssDNA into the cytoplasm; SOS induction and chromosomal DNA repair; activation of lux genes by the fused recA promoter carried on a plasmidal DNA; transcription and translation of the luminescence responsible enzymes; luminescence cycle; energy molecules level regulation and the regulation of the O2 consumption. The mathematical model was defined using a set of ordinary differential equations (ODE) and solved numerically. We simulated the system for different concentrations of NA in water for specific biosensors concentration, and under limited O2 conditions. The simulated results were compared to experimental data and satisfactory matching was obtained. This manuscript presents a proof of concept showing that real biosensors can be modeled and simulated. This sets the ground to the next stage of implementing a comprehensive physiological model using experimentally extracted parameters. Following the completion of the next stage, it will be possible to construct a “Computer Aided Design” tool for the simulation of the genetically engineered biosensors. We define a term “bioCAD” for a Biological System Computer Aided Design. The specific bioCAD that is described here is aimed towards whole cell biosensors which are under investigation today for functional sensing. Usage of the bioCAD will improve the biosensors design process and boost their performance. It will also reduce Non Recurring Engineering (NRE) cost and time. Finally, using a parameterized solution will allow fair and quick evaluation of whole cell biosensors for various applications

Childhood obesity: State of art and future research directions

[EN] According to the World Health Organization (WHO), the childhood obesity affects 42 million children worldwide, 35 million of whom live in the developing countries (WHO, 2010). It is not only a social issue but also a political and economic problem that affects a large, vulnerable and strategic population. Because of its multifactorial origin requires a multisectoral approach. The aim of this study is to frame a multidisciplinary state of the art on the issue from a social view. Here is a literature review that considers all the relevant research, both international and national, with a striking attention to those published in the last five years. It is expected to clarify agreements and dissonances in the proposals to combat and prevent childhood obesity. Furthermore, this study aims to project recommendations for future studies involving childhood o[ES] La obesidad infantil, según la Organización Mundial de la Salud (OMS), afectaba en 2010 a 42 millones de niños en todo el mundo, 35 millones de los cuales viven en países en desarrollo (WHO, 2010).Se trata de un problema social, político y económico que afecta a un sector amplio, vulnerable y estratégico de la población. Dado su origen multifactorial, requiere de una atención multisectorial.El objetivo de este estudio es enmarcar un estado de la cuestión multidisciplinar sobre la cuestión desde una mirada social. Se realiza una revisión documental que considera todas aquellas investigaciones relevantes, tanto a nivel internacional como nacional, realizadas hasta el momento y atendiendo especialmente a las publicadas en los últimos cinco años.Se espera esclarecer acuerdos y disonancias en las propuestas de lucha y prevención de la obesidad infantil así como en la atribución de culpabilidades y causas con las que se ha venido asociando la enfermedad, proyectando recomendaciones para futuros estudios que abarquen la obesidad infantil.García Cortés, B. (2016). La obesidad infantil: estado de la cuestión y posibles líneas de investigación futura. Revista sobre la infancia y la adolescencia. (10):77-97. doi:10.4995/reinad.2015.3718SWORD77971