Visualizing the Distribution of Synapses from Individual Neurons in the Mouse Brain

BACKGROUND:Proper function of the mammalian brain relies on the establishment of highly specific synaptic connections among billions of neurons. To understand how complex neural circuits function, it is crucial to precisely describe neuronal connectivity and the distributions of synapses to and from individual neurons. METHODS AND FINDINGS:In this study, we present a new genetic synaptic labeling method that relies on expression of a presynaptic marker, synaptophysin-GFP (Syp-GFP) in individual neurons in vivo. We assess the reliability of this method and use it to analyze the spatial patterning of synapses in developing and mature cerebellar granule cells (GCs). In immature GCs, Syp-GFP is distributed in both axonal and dendritic regions. Upon maturation, it becomes strongly enriched in axons. In mature GCs, we analyzed synapses along their ascending segments and parallel fibers. We observe no differences in presynaptic distribution between GCs born at different developmental time points and thus having varied depths of projections in the molecular layer. We found that the mean densities of synapses along the parallel fiber and the ascending segment above the Purkinje cell (PC) layer are statistically indistinguishable, and higher than previous estimates. Interestingly, presynaptic terminals were also found in the ascending segments of GCs below and within the PC layer, with the mean densities two-fold lower than that above the PC layer. The difference in the density of synapses in these parts of the ascending segment likely reflects the regional differences in postsynaptic target cells of GCs. CONCLUSIONS:The ability to visualize synapses of single neurons in vivo is valuable for studying synaptogenesis and synaptic plasticity within individual neurons as well as information flow in neural circuits

Early Life Events Carry Over to Influence Pre-Migratory Condition in a Free-Living Songbird

Conditions experienced during development can have long-term consequences for individual success. In migratory songbirds, the proximate mechanisms linking early life events and survival are not well understood because tracking individuals across stages of the annual cycle can be extremely challenging. In this paper, we first use a 13 year dataset to demonstrate a positive relationship between 1st year survival and nestling mass in migratory Savannah sparrows (Passerculus sandwichensis). We also use a brood manipulation experiment to show that nestlings from smaller broods have higher mass in the nest relative to individuals from larger broods. Having established these relationships, we then use three years of field data involving multiple captures of individuals throughout the pre-migratory period and a multi-level path model to examine the hypothesis that conditions during development limit survival during migration by affecting an individual's ability to accumulate sufficient lean tissue and fat mass prior to migration. We found a positive relationship between fat mass during the pre-migratory period (Sept–Oct) and nestling mass and a negative indirect relationship between pre-migratory fat mass and fledging date. Our results provide the first evidence that conditions during development limit survival during migration through their effect on fat stores. These results are particularly important given recent evidence showing that body condition of songbirds at fledging is affected by climate change and anthropogenic changes to landscape structure

The 322 ka Tiribí Tuff: stratigraphy, geochronology and mechanisms of deposition of the largest and most recent ignimbrite in the Valle Central, Costa Rica

The Tiribí Tuff covered much of the Valle Central of Costa Rica, currently the most densely populated area in the country (∼2.4 million inhabitants). Underlying the tuff, there is a related well-sorted pumice deposit, the Tibás Pumice Layer. Based on macroscopic characteristics of the rocks, we distinguish two main facies in the Tiribí Tuff in correlation to the differences in welding, devitrification, grain size, and abundance of pumice and lithic fragments. The Valle Central facies consists of an ignimbritic plateau of non-welded to welded deposits within the Valle Central basin and the Orotina facies is a gray to light-bluish gray, densely to partially welded rock, with yellowish and black pumice fragments cropping out mainly at the Grande de Tárcoles River Gorge and Orotina plain. This high-aspect ratio ignimbrite (1:920 or 1.1×10−3) covered an area of at least 820 km2 with a long runout of 80 km and a minimum volume outflow of 25 km3 (15 km3 DRE). Geochemically, the tuff shows a wide range of compositions from basaltic-andesites to rhyolites, but trachyandesites are predominant. Replicate new 40Ar/39Ar age determinations indicate that widespread exposures of this tuff represent a single ignimbrite that was erupted 322±2 ka. The inferred source is the Barva Caldera, as interpreted from isopach and isopleth maps, contours of the ignimbrite top and geochemical correlation (∼10 km in diameter). The Tiribí Tuff caldera-forming eruption is interpreted as having evolved from a plinian eruption, during which the widespread basal pumice fall was deposited, followed by fountaining pyroclastic flows. In the SW part of the Valle Central, the ignimbrite flowed into a narrow canyon, which might have acted as a pseudo-barrier, reflecting the flow back towards the source and thus thickening the deposits that were filling the Valle Central depression. The variable welding patterns are interpreted to be a result of the lithostatic load and the influence of the content and size of lithic fragments

Nature and significance of small volume fall deposits at composite volcanoes: Insights from the October 14, 1974 Fuego eruption, Guatemala

The first of four successive pulses of the 1974 explosive eruption of Fuego volcano, Guatemala, produced a small volume (∼0.02 km3 DRE) basaltic sub-plinian tephra fall and flow deposit. Samples collected within 48 h after deposition over much of the dispersal area (7–80 km from the volcano) have been size analyzed down to 8 φ (4 µm). Tephra along the dispersal axis were all well-sorted (σ φ = 0.25–1.00), and sorting increased whereas thickness and median grain size decreased systematically downwind. Skewness varied from slightly positive near the vent to slightly negative in distal regions and is consistent with decoupling between coarse ejecta falling off the rising eruption column and fine ash falling off the windblown volcanic cloud advecting at the final level of rise. Less dense, vesicular coarse particles form a log normal sub-population when separated from the smaller (Mdφ < 3φ or < 0.125 mm), denser shard and crystal sub-population. A unimodal, relatively coarse (Mdφ = 0.58φ or 0.7 mm σ φ = 1.2) initial grain size population is estimated for the whole (fall and flow) deposit. Only a small part of the fine-grained, thin 1974 Fuego tephra deposit has survived erosion to the present day. The initial October 14 pulse, with an estimated column height of 15 km above sea level, was a primary cause of a detectable perturbation in the northern hemisphere stratospheric aerosol layer in late 1974 to early 1975. Such small, sulfur-rich, explosive eruptions may substantially contribute to the overall stratospheric sulfur budget, yet leave only transient deposits, which have little chance of survival even in the recent geologic record. The fraction of finest particles (Mdφ = 4–8φ or 4–63 µm) in the Fuego tephra makes up a separate but minor size mode in the size distribution of samples around the margin of the deposit. A previously undocumented bimodal–unimodal–bimodal change in grain size distribution across the dispersal axis at 20 km downwind from the vent is best accounted for as the result of fallout dispersal of ash from a higher subplinian column and a lower “co-pf” cloud resulting from pyroclastic flows. In addition, there is a degree of asymmetry in the documented grain-size fallout pattern which is attributed to vertically veering wind direction and changing windspeeds, especially across the tropopause. The distribution of fine particles (<8 µm diameter) in the tephra deposit is asymmetrical, mainly along the N edge, with a small enrichment along the S edge. This pattern has hazard significance