Electrochemical reduction of oxygen catalyzed by Pseudomonas aeruginosa

Pseudomonas aeruginosa has already been shown to catalyze oxidation processes in the anode compartment of a microbial fuel cell. The present study focuses on the reverse capacity of the bacterium, i.e. reduction catalysis. Here we show that P. aeruginosa is able to catalyze the electrochemicalreduction of oxygen. The use of cyclic voltammetry showed that, for a given range of potential values, the current generated in the presence of bacteria could reach up to four times the current obtained without bacteria. The adhesion of bacteria to the working electrode was necessary for the catalysis to be observed but was not sufficient. The electron transfer between the working electrode and the bacteria did not involve mediator metabolites like phenazines. The transfer was by direct contact. The catalysis required a certain contact duration between electrodes and live bacteria but after this delay, the metabolic activity of cells was no longer necessary. Membrane-bound proteins, like catalase, may be involved. Various strains of P. aeruginosa, including clinical isolates, were tested and all of them, even catalase-defective mutants, presented the same catalytic property. P. aeruginosa offers a new model for the analysis of reduction catalysis and the protocol designed here may provide a basis for developing an interesting tool in the field of bacterial adhesion

Present Status of Introductions and Spread Through Europe of Alien Pests Associated with Woody Plants (Abstract)

A recent analysis of a worldwide database of the dates of introduction of more than 17 000 animal and plant species into continents other than their native one shows that, in most groups — in particular insects and other arthropods — establishment of new species is not saturated. With globalisation has brought about an exponential increase in the rate of arrival of new alien species of terrestrial invertebrates in Europe, rising from 7 to nearly 20 per year as between the first half of the last century and today. The increase is mainly due to the arrival of plant-eating invertebrates (11.5/year) whereas the other groups are tending to regress. These plant-eating invertebrates are also significantly more associated with woody plants (8 new species per year) than with herbaceous ones. Just over 480 species, essentially insects plus some mites and nematodes, have become established on woody plants in Europe since 1800. The arrival of so-called emerging species, not known to be invasive elsewhere, is a main feature of the latest period bringing to the fore the need to define identification tools for potential invaders. The pathways and vectors of the invasion have moved to trade in ornamental plants and to a lesser degree wooden packaging, while trade in timber is more limited with just a few species having been introduced via this pathway. Furthermore, Asia has become the main source of these introductions. Another characteristic feature of the current period is the acceleration in the species’ speed of propagation once they are established. The species detected in Europe after 1990 spread approximately 3 to 4 times faster than those that had arrived earlier. The cause of this appears to be the combination of changes in economic policies implemented in Europe with the explosion of the trade in ornamental plants, particularly shrubs. nearly half of these pests confine themselves to their original alien plant, but an analysis of the variation over time in colonisation of the large groups of host woody plants shows up a recent acceleration in new species associated with eucalyptus, palm trees and other woody plants originating from regions with warm climates, suggesting that there is a connection with global warming

Catalysis of the electrochemical reduction of oxygen by bacteria isolated from electro-active biofilms formed in seawater

Biofilmsformed in aerobic seawater on stainless steel are known to be efficient catalysts of the electrochemicalreduction of oxygen. Based on their genomic analysis, seven bacterial isolates were selected and a cyclic voltammetry (CV) procedure was implemented to check their electrocatalytic activity towards oxygenreduction. All isolates exhibited close catalytic characteristics. Comparison between CVs recorded with glassy carbon and pyrolytic graphite electrodes showed that the catalytic effect was not correlated with the surface area covered by the cells. The low catalytic effect obtained with filtered isolates indicated the involvement of released redox compounds, which was confirmed by CVs performed with adsorbed iron–porphyrin. None of the isolates were able to form electro-activebiofilms under constant polarization. The capacity to catalyze oxygenreduction is shown to be a widespread property among bacteria, but the property detected by CV does not necessarily confer the ability to achieve stable oxygenreduction under constant polarization

Worldwide tests of generic attractants, a promising tool for early detection of non-native cerambycid species

A large proportion of the insects which have invaded new regions and countries are emerging species, being found for the first time outside their native range. Being able to detect such species upon arrival at ports of entry before they establish in non-native countries is an urgent challenge. The deployment of traps baited with broad-spectrum semiochemical lures at ports-of-entry and other high-risk sites could be one such early detection tool. Rapid progress in the identification of semiochemicals for cerambycid beetles during the last 15 years has revealed that aggregation-sex pheromones and sex pheromones are often conserved at global levels for genera, tribes or subfamilies of the Cerambycidae. This possibly allows the development of generic attractants which attract multiple species simultaneously, especially when such pheromones are combined into blends. Here, we present the results of a worldwide field trial programme conducted during 2018-2021, using traps baited with a standardised 8-pheromone blend, usually complemented with plant volatiles. A total of 1308 traps were deployed at 302 sites covering simultaneously or sequentially 13 European countries, 10 Chinese provinces and some regions of the USA, Canada, Australia, Russia (Siberia) and the Caribbean (Martinique). We intended to test the following hypotheses: 1) if a species is regularly trapped in significant numbers by the blend on a continent, it increases the probability that it can be detected when it arrives in other countries/continents and 2) if the blend exerts an effective, generic attraction to multiple species, it is likely that previously unknown and unexpected species can be captured due to the high degree of conservation of pheromone structures within related taxa. A total of 78,321 longhorned beetles were trapped, representing 376 species from eight subfamilies, with 84 species captured in numbers greater than 50 individuals. Captures comprised 60 tribes, with 10 tribes including more than nine species trapped on different continents. Some invasive species were captured in both the native and invaded continents. This demonstrates the potential of multipheromone lures as effective tools for the detection of 'unexpected' cerambycid invaders, accidentally translocated outside their native ranges. Adding new pheromones with analogous well-conserved motifs is discussed, as well as the limitations of using such blends, especially for some cerambycid taxa which may be more attracted by the trap colour or other characteristics rather than to the chemical blend.O

Understanding the trans-Neptunian Solar system: Reconciling the results of serendipitous stellar occultations and the inferences from the cratering record

The most pristine remnants of the Solar system's planet formation epoch orbit the Sun beyond Neptune, the small bodies of the trans-Neptunian object populations. The bulk of the mass is in ~100 km objects, but objects at smaller sizes have undergone minimal collisional processing, with New Horizons recently revealing that ~20 km effective diameter body (486958) Arrokoth appears to be a primordial body, not a collisional fragment. This indicates bodies at these sizes (and perhaps smaller) retain a record of how they were formed, and are the most numerous record of that epoch. However, such bodies are impractical to find by optical surveys due to their very low brightnesses. Their presence can be inferred from the observed cratering record of Pluto and Charon, and directly measured by serendipitous stellar occultations. These two methods produce conflicting results, with occultations measuring roughly ten times the number of ~km bodies inferred from the cratering record. We use numerical models to explore how these observations can be reconciled with evolutionary models of the outer Solar system. We find that models where the initial size of bodies decreases with increasing semimajor axis of formation, and models where the surface density of bodies increases beyond the 2:1 mean-motion resonance with Neptune can produce both sets of observations, though comparison to various observational tests favours the former mechanism. We discuss how to evaluate the astrophysical plausibility of these solutions, and conclude extended serendipitous occultation surveys with broad sky coverage are the most practical approach.Comment: Resubmitted to Astronomy & Astrophysics with revisions after referee's comments. Likely to be the first author's last paper; it's been an honour working with all of yo

The stone pine, Pinus pinea L., a new highly rewarding host for the invasive Leptoglossus occidentalis

Research ArticleThe invasive seed bug Leptoglossus occidentalis, a species native to Western North America, is of major concern for the producers of stone pine seeds in the Mediterranean countries. The large size of these edible seeds and their nutritive content may represent a pull factor for the seed bug. Cone and seed traits of three main Mediterranean pine species: P. pinea, P. pinaster, and P. halepensis, were evaluated. Preference trials with cone-bearing branches, individual cones and seeds were conducted to test host preference among the three host species. Considering the kernel size, stone pine seeds provide 4 to 13 times more reward than P. pinaster and P. halepensis seeds, respectively, but also needed a greater effort to be reached as measured by coat thickness. Still, the benefit/cost ratio was higher on P. pinea. Individual seeds and cones of P. pinea were 2 to 3 times more consumed than those of the two other pine species. However, branch preference trials did not reveal any difference in bug visits. Moreover, adults manifested strong group behaviour on branches, frequently dissociating into two persisting groups. The implications of these results for P. pinea producing areas are discussedinfo:eu-repo/semantics/publishedVersio