Production and vegetative growth of coffee trees under fertilization and shade levels

It is difficult to separate the effects of light reduction versus nutrient and water competition in agroforestry systems. The objective of this experiment was to evaluate the effect of shading and fertilizer supply on the vegetative development and yield of Coffea arabica over six years. The coffee trees were covered with shade screens (photosynthetically active radiation reduction up to 48%) and fertilized from 100 to 40% of the recommended amount from 2001 on. Leaf area, number of leaves, number of nodes, leaf area per branch and, yield were determined. Although no effect of fertilization was found, shading influenced the number of nodes, leaf area and production from the third year on. The number of nodes and yield decreased as shading increased. The effect of the yield bienniallity was more evident in the unshaded trees, which yielded an average of 2,646 kg ha¹. The trees under 48% shading yielded an average of 2,094 kg ha¹. After 2004 the leaf area per branch increased as the shade increased, at the end of both the maximum and minimum growth periods. During the first three years, the coffee trees do not change their vegetative or productive characteristics as a response to shading. The shade effects become more intense after the beginning of the higher yield period. The shaded coffee trees have a larger leaf area and a smaller number of nodes than the coffee trees under full sun. The yield decreases as photosynthetically active radiation limitation increases, and yield bienniallity is less intense in shaded trees.Em sistemas agroflorestais é difícil separar os efeitos da competição por luz daquela por água e nutrientes. O objetivo do experimento foi avaliar o efeito do sombreamento e do fornecimento de fertilizantes sobre o desenvolvimento vegetativo e a produtividade de cafeeiros arábica ao longo de seis anos. Os cafeeiros foram cobertos com telas sombreadoras (redução da radiação fotosinteticamente ativa em até 48%) e fertilizados de 100 até 40% da recomendação. Foram determinados a área da folha, o número de folhas, o número de nós, a área foliar por ramo e a produtividade dos cafeeiros. Embora não houvesse efeito da fertilização, o sombreamento influenciou o número de nós, a área da folha e a produção a partir do terceiro ano. O número de nós e a produtividade reduziram conforme aumentou o sombreamento. Cafeeiros sob pleno sol apresentaram maior bienalidade da produção e produtividade média de 2646 kg ha¹. Cafeeiros sob 48% de sombreamento produziram em média 2094 kg ha¹. Após três anos a área foliar aumentou com o aumento do sombreamento, tanto no final do período de maior quanto de menor crescimento. Nos três primeiros anos, os cafeeiros não diferiram nas características vegetativas ou reprodutivas devido ao sombreamento. Os efeitos do sombreamento tornam-se mais intensos após o início do período de altas produtividades. Cafeeiros sombreados apresentam folhas maiores e menor número de nós do que cafeeiros sob pleno sol. Nos anos de alta produtividade esta decresce com o aumento do sombreamento e a bienalidade é menos intensa nos cafeeiros sombreados

Effectiveness of the combination elvitegravir/cobicistat/tenofovir/emtricitabine (EVG/COB/TFV/FTC) plus darunavir among treatment-experienced patients in clinical practice : A multicentre cohort study

Background: The aim of this study was to investigate the effectiveness and tolerability of the combination elvitegravir/cobicistat/tenofovir/emtricitabine plus darunavir (EVG/COB/TFV/FTC + DRV) in treatment-experienced patients from the cohort of the Spanish HIV/AIDS Research Network (CoRIS). Methods: Treatment-experienced patients starting treatment with EVG/COB/TFV/FTC + DRV during the years 2014-2018 and with more than 24 weeks of follow-up were included. TFV could be administered either as tenofovir disoproxil fumarate or tenofovir alafenamide. We evaluated virological response, defined as viral load (VL) < 50 copies/ml and < 200 copies/ml at 24 and 48 weeks after starting this regimen, stratified by baseline VL (< 50 or ≥ 50 copies/ml at the start of the regimen). Results: We included 39 patients (12.8% women). At baseline, 10 (25.6%) patients had VL < 50 copies/ml and 29 (74.4%) had ≥ 50 copies/ml. Among patients with baseline VL < 50 copies/ml, 85.7% and 80.0% had VL < 50 copies/ml at 24 and 48 weeks, respectively, and 100% had VL < 200 copies/ml at 24 and 48 weeks. Among patients with baseline VL ≥ 50 copies/ml, 42.3% and 40.9% had VL < 50 copies/ml and 69.2% and 68.2% had VL < 200 copies/ml at 24 and 48 weeks. During the first 48 weeks, no patients changed their treatment due to toxicity, and 4 patients (all with baseline VL ≥ 50 copies/ml) changed due to virological failure. Conclusions: EVG/COB/TFV/FTC + DRV was well tolerated and effective in treatment-experienced patients with undetectable viral load as a simplification strategy, allowing once-daily, two-pill regimen with three antiretroviral drug classes. Effectiveness was low in patients with detectable viral loads