In Vitro Propagation Of Sacha Inchi [propagação In Vitro De Sacha Inchi]

The aim of this study was to perform an in vitro evaluation of the auxin:cytokinine ratio in different segments of the epicotyl and hypocotyl of Sacha inchi (Plukenetia Volubilis Linneo) seeds germinated in vitro. The segments apical (A), median (B) and basal (C) were introduced into semi-solid MS culture medium (2.0g L -1 Phytagel), supplemented with MS vitamins, sucrose (30.0g L -1) and submitted to three doses of auxin indolebutyric acid - IBA (0; 0.1; 0.5mg L -1), associated with four doses of the cytokinine benzylaminopurine - BAP (0; 0.1; 0.5; 1.0mg L -1), totaling 36 treatments. After nine weeks of in vitro cultivation, the apical segment (A) presented shoot formation by direct organogenesis at the concentrations of 0.5 and 1.0 of BAP associated with 0.0 and 0.1 of IBA. It is feasible to use in vitro cultivation with the apical region of seeds germinated in vitro used as explants.42711681172Céspedes, E.I.M., (2006) Cultivo de Sacha Inchi, p. 11. , Tarapoto, San Martin, Peru: INIIA, Subdirección De Recursos Geneticos Y BiotecnologíaDimech, A.M., Micropropagation of Gymea Lily (Doryanthes excelsa Corrêa) from New South Wales, Australia (2007) Plant Cell, Tissue and Organ Culture, 88, pp. 157-165. , http://www.springerlink.com/content/k070lrjll72x370g/fulltext.html, 9188. Disponível em:,. Acesso em: 02 fev. 2007. doi: 10.1007/s11240-006--1Guillén, M.D., Characterization of Sacha Inchi (Plukenetia volubilis L.) Oil by FTIR Spectroscopy and 1H NMR. Comparison with Linseed Oil (2003) Journal of the American Oil Chemist Society, 80, pp. 755-762Huamán, J., Efecto de la Plukenetia volubilis Linneo (Sacha inchi) en la trigliceridemia posprandial (2008) Annales Faculdad Medicina, 69, pp. 263-266Jesus, A.M.S., Cultivo in vitro de embriões zigóticos de Jatropha (2003) Revista Ceres, 50, pp. 183-189Lima, G.P.P., Efeito do BAP e ANA e atividade da peroxidase em mandioca (Manihot esculenta Crantz cv Mcol 22) cultivada in vitro (2002) Revista Brasileira de Agrociência, 8 (2), pp. 127-130Mala, J., Role of phytohormones in organogenic ability of elm multiplicated shoots (2005) Biologia Plantarum, 50, pp. 8-14Murashige, T., Skoog, F., A revised medium for rapid growth and bioassays with tobacco tissue cultures (1962) Physiologia Plantarum, 15, pp. 473-497Nunes, C.F., Diferentes suplementos no cultivo in vitro de embriões de pinhão manso (2008) Pesquisa agropecuária brasileira, 43 (1), pp. 9-14Vazquez Molina, D.E., Sugar cane buds as an efficient explant for plantlet regeneration (2005) Biologia Plantarum, 49, pp. 481-485. , http://springerlink.com/content/l165h14730u55466/, 2005. Disponível em:,. Acesso em: 03 se

Horticultural Performance Of In Vitro Propagated Plants Of Sacha Inchi [desempenho Horticultural De Plantas Propagadas In Vitro De Sacha Inchi]

The aim of this study was to evaluate, in the field, the horticultural characteristics of Sacha inchi (Plukenetia volubilis L.) in vitro plants considering time of flowering and harvesting, the productivity, as well as their morphological characteristics: color and shape of leaves, stems and fruits. The plantlets, originated from the apex of in vitro plants, were propagated in culture medium with MS salts and vitamins, add 30g L-1 sucrose and 1.0mg L-1 BAP for six weeks. Were selected at random, four plants propagated in vitro, four plants by seminal and twenty-two plants, also by composing the border, totaling thirty plants. Analyses were completed weekly until fourteen months after planting, which occurred before the first pruning. The results indicate the non-occurrence of genetic variability in vitro grown plants, as well as early in the production and increased productivity as compared with the conventional material obtained via seminal.44610501053Bordignon, S.R., Propagação in vitro de Sacha inchi (Plukenetia volubilis Linneo) (2012) Ciência Rural, 42 (7), pp. 1168-1172. , http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103_84782012000700005&lng=en&nrm=iso, cesso em: 05 Feb. 2014. Doi: 10.1590/S0103-84782012005000049Céspedes, E.I.M., (2006) Cultivo De Sacha Inchi, p. 11. , Tarapoto, San Martin, Peru: INIIA, Subdirección De Recursos Geneticos Y BiotecnologíaGuillén, M.D., Characterization of Sacha Inchi (Plukenetia volubilis L.) oil by FTIR spectroscopy and 1H NMR. Comparison with linseed oil (2003) Journal of the American Oil Chemist Society, 80, pp. 755-762. , http://link.springer.com/article/10.1007%2Fs11746-003-0768-z#, Acesso em: 06 Feb. 2014. Doi: 10.1007/s11746-003-0768-zHuamán, J., Efecto de la Plukenetia volubilis Linneo (Sacha inchi) en la trigliceridemia posprandial (2008) Annales Faculdad Medicina, 69, pp. 263-266Murashige, T., Skoog, F., A revised medium for rapid growth and bioassays with tobacco tissue cultures (1962) Physiologia Plantarum, 15, pp. 473-497Rodrigues, P.H.V., Heliconia bihai var. Lobster Claw I: Cut flower field production from micropropagated- versus rhizome-derived plants (2006) Floriculture, Ornamental and Plant Biotechnology: Advances and Topical Issues, 2, pp. 558-560. , SILVA da, J.A.T. (Org, Londres: Global Science BooksRodrigues, P.H.V., Variação somaclonal em mudas micropropagadas de abacaxi ornamental, Ananas Bracteatus Schultes var. Striatus (2007) Revista Brasileira De Horticultura Ornamental, 12 (2), pp. 122-125. , http://132.248.9.34/hevila/Revistabrasileiradehorticulturaornamental/2006/vol12/no2/8.pdf, Acesso em: 06 Feb. 2014Rodrigues, P.H.V., Somaclonal variation in micropropagated Heliconia bihai cv. Lobster Claw I Plantlets (Heliconiaceae) (2008) Scientia Agricola, 65, pp. 681-684. , http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103_90162008000600017&lng=en&nrm=iso, Acesso em: 06 Feb. 2014. Doi: 10.1590/S0103-90162008000600017Santos, C.C.C., Rodrigues, P.H.V., Ocorrência de variação somaclonal em mudas de bananeira micropropagadas da cultivar Pacovan (Musa spp., grupo AAB) (2004) Revista Bragantia, 63 (2), pp. 201-205. , http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006_87052004000200005&lng=en&nrm=iso, Acesso em: 06 Feb. 2014. Doi: 10.1590/S0006-8705200400020000

Propagation Of Heliconia Plantlets In Temporarily Immersion Bioreactor [propagação De Mudas De Helicônia Em Biorreator De Imersão Temporária]

Novel micropropagation techniques have been studied aiming the optimization of production and cost reduction. Among these micropropagation using temporarily immersion in a bioreactor is a technique that uses liquid medium in an automatzed system. This research evaluated the efficacy of a handcraft temporarily immersion system, using explants of Heliconia champneiana Griggs cv. Splash. These were submitted to three subcultures with fifteen minutes of immersion in intervals of one hour (T1), four hours (T4), six hours (T6) and eight hours (T8). Weight of the fresh mass, number of plantlets, individual weight, final volume of culture media, pH final and the overall functionality were evaluated. In evaluations between the treatments of temporarily immersion, the best performance occurred in treatment T1, being impracticable the T8 treatment for this type culture. In comparison to a conventional method (C) the treatments T1 and T4 had superior performance, demonstrating higher efficiency of the temporarily immersion for the production of plantlets. The prototype of the bioreactor of temporary immersion had good performance, presenting itself functional, of easy handling and able to be used in the study of other cultures.6512935Aitken-Christie, J., Jones, C., Towards automation: Radiata pine shoot hedges in vitro (1987) Plant Cell, Tissue and Organ Culture, 8, pp. 185-196. , DordrechtAlvard, D., Cote, F., Teisson, C., Comparasion of methods of liquid medium culture for banana micropropagation (1993) Plant Cell, Tissue and Organ Culture, 32, pp. 55-60. , DordrechtCastro, C.E.F., Graziano, T.T., Espécies do gênero Heliconia (Heliconiaceae) no Brasil (1997) Revista Brasileira De Horticultura Ornamental, 3, pp. 15-28. , CampinasDias, M.A.S., Rodrigues, P.H.V., Fontes de explantes e contaminantes isolados em cultivo in vitro de Heliconia bihai (HELICONIACEAE) (2001) Revista Brasileira De Horticultura Ornamental, 7, pp. 165-168. , CampinasDiniz, J.D.N., Gonçalves, A.N., Minami, K., Variação do pH no meio de cultura in vitro para explantes de bananeira cv. Prata Anã (1996) Ciência Agronômica, 27 (1-2), pp. 87-91. , FortalezaGomes, F.P., (1987) Curso De Estatística Experimental, p. 466. , 12.ed. São Paulo: NobelLamas, A.M., (2001) Floricultura Tropical: Técnicas De Cultivo, p. 88. , Recife: SEBRAE/PELemos, E.E.P., Ferreira, M.S., Alencar, L.M.C., Oliveira, J.G.L., Magalhães, V.S., Micropropagação de clones de banana cv. Terra em biorreator de imersão temporária (2001) Revista Brasileira De Fruticultura, 23 (3), pp. 482-487. , JaboticabalLorenzo, J.C., González, B.L., Escalona, M., Teisson, C., Espinosa, P., Borroto, C., Sugarcane shoot formation in an improved temporary immersion system (1998) Plant Cell, Tissue and Organ Culture, 54, pp. 197-200. , DordrechtMorel, G., Wetmore, R.H., Tissue culture of monocotyledons (1951) American Journal of Botany, 38, pp. 138-140. , IthacaMurashige, T., Skoog, F., A revised medium for rapid growth and bioassays with tabacco tissue cultures (1962) Plant Physiology, 15, pp. 473-497. , CopenhagenRodrigues, P.H.V., In vitro establishment of Heliconia rauliniana (Heliconiaceae) (2005) Scientia Agrícola, 62 (1), pp. 69-71. , PiracicabaRodrigues, P.H.V., Lima, A.M.L.P., Ambrosano, G.M.B., Dutra, M.F.B., Acclimatization of micropropagated Heliconia bihai (Heliconiaceae) plants (2005) Scientia Agrícola, 62 (3), pp. 288-290. , Piracicaba(2001), SAS Institute, Release 8.2, Cary, NC, USASkirvin, R.M., Chu, M.C., Mann, M.L., Young, H., Sullivan, J., Fermanian, T., Stability of tissue culture medium pH as a function of autoclaving, time, and cultured plant material (1986) Plant Cell Reports, 5, pp. 292-294. , BerlinTeisson, C., Alvard, D., A new concept of plant in vitro cultivation in liquid medium: Temporary immersion (1994) VIII International Congress of Plant Tissue and Cell Culture, p. 54. , 1994, Florence. Abstract... Florence: ICPTCCWilliams, R.R., Taji, A.M., Winnel, K.A., The effect of Ptilotus plant tissue on pH of in vitro media (1990) Plant Cell Tissue and Organ Culture, 22, pp. 153-158. , Dordrech

Somaclonal variation in micropropagated Heliconia bihai cv. Lobster Claw I plantlets (Heliconiaceae) Variação somaclonal em mudas micropropagadas de Helicônia, Heliconia Bihai cv. Lobster Claw I (Heliconiaceae)

The occurrence of somaclonal variation is described in various cultures of agronomic interest. Such variation can be of benefit in the development of new flower varieties. In this study, the occurrence of somaclonal variation in micropropagated changes of Heliconia bihai cv. Lobster Claw I was investigated. Stem apexes were introduced in MS culture media with the addition of 2.5 mg L-1 of benzylaminopure (BAP) and 500 mg L-1 of sodium cefotaxime. After selecting the apex stem, it was sub-cultivated in MS media and supplemented with 4.0 mg L-1 of BAP to induce side buds. To conduct the trial, 2,000 plants were selected and compared with plants originated from rhizomes. To calculate the percentage of the variants, the plant stature, the form and color of leaves and pseudostem were evaluated. The plants with buds presenting the same type of variation were considered as variants. The occurrence of three types of somaclonal variants was observed: Variation of the Chlorophyll in the Leaf, Low Stature Variant and Pseudostem and Petiole Color Variant, the latter with ornamental potential. The somaclonal variation rate for Heliconia bihai cv Lobster Claw I, under the proposed conditions, was 61.40%.<br>A ocorrência de variação somaclonal é descrita em diversas culturas de interesse agronômico. A floricultura pode beneficiar-se dessa variabilidade, com a obtenção de novas variedades. Nesse trabalho, estudou-se a ocorrência de variação somaclonal em mudas micropropagadas de Heliconia bihai cv. Lobster Claw I. Ápices caulinares foram introduzidos em meio de cultivo MS com adição de 2,5 mg L-1 de benzilaminopurina (BAP) e 500 mg L-1 de cefotaxima sódica. Após a seleção do ápice caulinar, o explante foi subcultivado em meio MS suplementado com 4,0 mg L-1 de BAP para indução de brotações. Foram selecionadas, ao acaso, 2.000 mudas e comparadas com mudas originadas de rizomas, para compor o ensaio. No cálculo da porcentagem dos variantes avaliaram-se as características: estatura da planta, a forma e coloração das folhas e pseudocaule. Consideraram-se como variantes as plantas cujos perfilhos também mostravam o mesmo tipo de variação. Constatou-se a ocorrência de três tipos de variantes somaclonais, VCF (Variação da Clorofila na Folha), VPB (Variante de Porte Baixo) e VCPP (Variante da Coloração do Pseudocaule e Pecíolo), este último com potencial ornamental. A taxa de variação somaclonal para Heliconia bihai cv Lobster Claw I, nas condições propostas, foi de 61,40 %

In Vitro Propagation Of Astrocarium Aculeatum [propagação In Vitro De Tucumã Do Amazonas]

There is a large number of species within the genus Astrocaryum with the potential for use as food and for oil production. The most used species, tucumã do Amazonas, is consumed on a large scale in the States in the northern region of Brazil, originating from extractivism. Zygotic embryos of mature and immature tucumã do Amazonas (Astrocaryum aculeatum G. Mey.) seeds were inoculated in a semi-solid Murashige and Skoog (MS) culture medium supplemented with vitamins. The surviving embryos were transferred to the same MS medium supplemented with 0.0; 1.0; 3.0 and 5.0mg L-1 of BAP, obtaining an increasing rate of shoot formation.4315559Almedia, M., Pre-procambial cells are niches for pluripotent and totipotent stem-like cells for organogenesis and somatic embryogenesis in the peach palm: A histological study (2012) Plant Cell Reports, , http://rd.springer.com/article/10.1007/s00299-012-1264-6, Disponível em, Acesso em: 12 jun. 2012. doi: 10.1007/s00299-012-1264-6Elias, M.E.A., Emergência de plântulas de tucumã (Astrocaryum aculeatum) em função da posição de semeadura (2006) Acta Amazônica, 36 (3), pp. 385-388Ferreira, S.A.N., Gentil, D.F.O., Extração, embebição e germinação de sementes de tucumã (Astrocaryum aculeatum) (2005) Acta Amazônica, 36 (2), pp. 141-146Ferreira, S.A.N., Gentil, D.F.O., Morfologia da plântula em desenvolvimento de Astrocaryum aculeatum Meyer (Arecaceae) (2005) Acta Amazônica, 35 (3), pp. 337-342Franco, E.T.H., Ferreira, A.G., Tratamentos prégerminativos em sementes de Didymopanax morototoni (Aubl.) Dcne. et Planch (2002) Ciência Florestal, 12 (1), pp. 1-10Graner, E.M., (2009) Avaliações Morfofisiológicas Do Desenvolvimento De Microplantas De Pupunheiras Submetidas a Tratamentos Com Biorreguladores, p. 242. , http://www.teses.usp.br/teses/disponiveis/11/11144/tde-09092009-100448, Dissertação (Mestrado em Fisiologia e Bioquímica de Plantas) - Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba, SP. Disponível em, Acesso em: 15 jun. 2012Hu, C.Y., Ferreira, A.G., Cultura De Embriões (1998) Cultura De Tecidos E Transformação Genética De Plantas, 1, pp. 371-393. , Brasília, DF: EMBRAPA-SPI/ EMBRAPA-CNPHMurashige, T., Skoog, F., A revised medium for rapid growth and bioassays with tobacco tissue culture (1962) Physiologia Plantarum, 15, pp. 473-497Pereira, J.E.S., Germinação in vitro de embriões zigóticos de murmuru (Astrocaryum ulei) (2006) Ciência Agrotecnológica, 30 (2), pp. 251-256Pierik, R.L.M., (1990) Cultivo In Vitro De Las Plantas Superiores, p. 326. , 3.ed. Madrid: Mundi-Prensa(2008), p. 620. , SAS - Statistical Analysis System:.Realease 9.1.3, (software). CarryTaiz, L., Zeiger, E., (2006) Plant Physiology, p. 764. , 4.ed. Massachusetts: Sinauer AssociatesYuyama, L.K.O., Processamento e avaliação da vidade- prateleira do tucumã (Astrocaryum aculeatum Meyer) desidratado e pulverizado (2008) Ciência Tecnologia Dos Alimentos, 28 (2), pp. 408-41