91 research outputs found
Optical Identification of He White Dwarfs Orbiting Four Millisecond Pulsars in the Globular Cluster 47 Tucanae
We used ultra-deep UV observations obtained with the Hubble Space Telescope
to search for optical companions to binary millisecond pulsars (MSPs) in the
globular cluster 47 Tucanae. We identified four new counterparts (to MSPs
47TucQ, 47TucS, 47TucT and 47TucY) and confirmed those already known (to MSPs
47TucU and 47TucW). In the color magnitude diagram, the detected companions are
located in a region between the main sequence and the CO white dwarf cooling
sequences, consistent with the cooling tracks of He white dwarfs of mass
between 0.15 Msun and 0.20 Msun. For each identified companion, mass, cooling
age, temperature and pulsar mass (as a function of the inclination angle) have
been derived and discussed. For 47TucU we also found that the past accretion
history likely proceeded in a sub-Eddington rate. The companion to the redback
47TucW is confirmed to be a non degenerate star, with properties particularly
similar to those observed for black widow systems. Two stars have been
identified within the 2-sigma astrometric uncertainty from the radio positions
of 47TucH and 47TucI, but the available data prevent us from firmly assessing
whether they are the true companions of these two MSPs.Comment: 27 pages, 7 figures, Accepted for publication by Ap
Radio Timing and Optical Photometry of the Black Widow System PSR J1953+1846A in the Globular Cluster M71
We report on the determination of the astrometric, spin and orbital
parameters for PSR J1953+1846A, a "black widow" binary millisecond pulsar in
the globular cluster M71. By using the accurate position and orbital parameters
obtained from radio timing, we identified the optical companion in ACS/Hubble
Space Telescope images. It turns out to be a faint (m_F606W>=24, m_F814W>=23)
and variable star located at only ~0.06" from the pulsar timing position. The
light curve shows a maximum at the pulsar inferior conjunction and a minimum at
the pulsar superior conjunction, thus confirming the association with the
system. The shape of the optical modulation suggests that the companion star is
heated, likely by the pulsar wind. The comparison with the X-ray light curve
possibly suggests the presence of an intra-binary shock due to the interaction
between the pulsar wind and the material released by the companion. This is the
second identification (after COM-M5C) of an optical companion to a black widow
pulsar in a globular cluster. Interestingly, the two companions show a similar
light curve and share the same position in the color magnitude diagram.Comment: Accepted for publication by ApJ; 33 Pages, 10 Figures, 3 Table
Clinical Outcomes of Thirteen Patients with Acute Chagas Disease Acquired through Oral Transmission from Two Urban Outbreaks in Northeastern Brazil
Chagas disease is caused by a parasitic protozoan transmitted to humans by the contaminated feces of blood-feeding assassin bugs from the Triatominae subfamily. It may also be transmitted from mother to baby during pregnancy, by breastfeeding, blood transfusion or organ transplant. In rare cases, the disease can also be caused by accidental ingestion of contaminated food (sugar cane or açaà juice, drinking water, etc.). Acute Chagas disease often presents itself as a mononucleosis-like syndrome, with symptoms including fever, lymph node enlargement and muscle pain. The mortality rate of acute Chagas disease is high, mainly due to heart failure as a consequence of cardiac fiber lesions. There are few studies describing clinical outcomes and the disease progression of patients who receive therapeutic treatment, especially with regard to cardiac exam findings. In this report, the authors describe clinical findings from two micro-outbreaks occurring in impoverished towns in northeastern Brazil. Prior to receiving treatment, patient mortality rate was 28.6% in one of the outbreaks, and one pregnant woman experienced a spontaneous abortion due to the disease in the other outbreak. Most patients complained of fever, dyspnea, myalgia and periorbital edema. After receiving a two-month course of treatment, clinical symptoms improved and the number of abnormalities in cardiac exams decreased
DNA Vaccines against Dengue Virus Type 2 Based on Truncate Envelope Protein or Its Domain III
Two DNA vaccines were constructed encoding the ectodomain (domains I, II and III) of the DENV2 envelope protein (pE1D2) or only its domain III (pE2D2), fused to the human tissue plasminogen activator signal peptide (t-PA). The expression and secretion of recombinant proteins was confirmed in vitro in BHK cells transfected with the two plasmids, detected by immunofluorescence or immunoprecipitation of metabolically labeled gene products, using polyclonal and monoclonal antibodies against DENV2. Besides, results reveal that the ectodomain of the E protein can be efficiently expressed in vivo, in a mammalian system, without the prM protein that is hypothesized to act as a chaperonin during dengue infection. Balb/c mice were immunized with the DNA vaccines and challenged with a lethal dose of DENV2. All pE1D2-vaccinated mice survived challenge, while 45% of animals immunized with the pE2D2 died after infection. Furthermore, only 10% of pE1D2-immunized mice presented some clinical signs of infection after challenge, whereas most of animals inoculated with the pE2D2 showed effects of the disease with high morbidity degrees. Levels of neutralizing antibodies were significantly higher in pE1D2-vaccinated mice than in pE2D2-immunized animals, also suggesting that the pE1D2 vaccine was more protective than the pE2D2
Colocalization of Protein Kinase A with Adenylyl Cyclase Enhances Protein Kinase A Activity during Induction of Long-Lasting Long-Term-Potentiation
The ability of neurons to differentially respond to specific temporal and spatial input patterns underlies information storage in neural circuits. One means of achieving spatial specificity is to restrict signaling molecules to particular subcellular compartments using anchoring molecules such as A-Kinase Anchoring Proteins (AKAPs). Disruption of protein kinase A (PKA) anchoring to AKAPs impairs a PKA-dependent form of long term potentiation (LTP) in the hippocampus. To investigate the role of localized PKA signaling in LTP, we developed a stochastic reaction-diffusion model of the signaling pathways leading to PKA activation in CA1 pyramidal neurons. Simulations investigated whether the role of anchoring is to locate kinases near molecules that activate them, or near their target molecules. The results show that anchoring PKA with adenylyl cyclase (which produces cAMP that activates PKA) produces significantly greater PKA activity, and phosphorylation of both inhibitor-1 and AMPA receptor GluR1 subunit on S845, than when PKA is anchored apart from adenylyl cyclase. The spatial microdomain of cAMP was smaller than that of PKA suggesting that anchoring PKA near its source of cAMP is critical because inactivation by phosphodiesterase limits diffusion of cAMP. The prediction that the role of anchoring is to colocalize PKA near adenylyl cyclase was confirmed by experimentally rescuing the deficit in LTP produced by disruption of PKA anchoring using phosphodiesterase inhibitors. Additional experiments confirm the model prediction that disruption of anchoring impairs S845 phosphorylation produced by forskolin-induced synaptic potentiation. Collectively, these results show that locating PKA near adenylyl cyclase is a critical function of anchoring
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