Cytomegalovirus (CMV) genotype in allogeneic hematopoietic stem cell transplantation

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Background: Based on sequence variation in the UL55 gene that encodes glycoprotein B (gB), human cytomegalovirus (CMV) can be classified into four gB genotypes. Previous studies have suggested an association between CMV gB genotype and clinical outcome in patients who underwent an allogeneic hematopoietic stem cell transplant (HSCT). The goals of this study were identify patients with active infection caused by CMV in recipients of HSCT; determine the prevalence of CMV genotypes in the study group; correlate genotype with CMV disease, acute GVHD and overall survival. Methods: The diagnosis of active CMV infection after allogeneic HSCT was detected by antigenemia (AGM) and/or nested-PCR (N-PCR). Positive samples from patients with active CMV infection were submitted to genotyping using N-PCR to amplify a region of UL55, followed by restriction analysis based on HinfI and RsaI digestion. Real-time PCR (qPCR) was used to determine the viral load during active CMV infection and antiviral treatment. Results: Sixty-three allogeneic HSCT recipients were prospectively evaluated; 49/63 (78%) patients were infected with CMV genotypes - gB1 19/49 (39%), gB2 17/49 (35%), gB3 3/49 (6%), gB4 7/49 (14%) - and 3 (6%) had mixed CMV genotypes (gB1 + gB3, gB1 + gB4 and gB2 + gB4). Characterized by gastrointestinal disease, CMV disease occurred in 3/49 (6.1%) patients, who had CMV gB3 genotype. These gB3 genotype patients presented an increasing AGM number, mean 125 (+/- 250) (P = 0.70), and qPCR copies/ml, mean 37938 (SD +/- 50542) (P = 0.03), during antiviral treatment, when compared with other CMV genotypes. According to CMV genotypes, stratified overall survival was 55% for gB1, 43% for gB2; 0% for gB3 and 57% for gB4 (P = 0.03). Conclusions: One of the restrictions of the presented study was the low number of CMV gB sub-cohorts). However, we demonstrated that the frequency of active CMV infection in this HSCT population was high, and the most prevalent genotype in these patients with active CMV infection was gB1 and gB2 genotype (74%). In Brazil, HSCT recipients seem to carry mainly gB1 and gB2 CMV genotype.13Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)FAPESP [07/52388-1

Prevalence of risk factors for breast neoplasm in the city of Maringá, Paraná state, Brazil Prevalencia de factores de riesgo para el cáncer de mama en el municipio de Maringá, en el estado de Paraná, Brasil Prevalência de fatores de risco para o câncer de mama no município de Maringá, Paraná, Brasil

The aim of this study was to identify the prevalence of the risk factors for breast cancer among women between 40 and 69 years old in the city of Maringá, Paraná State, Brazil. An analytical, exploratory and cross-sectional study was carried out through a population research at homes in Maringá. The sample was composed of 439 women between 40 and 69 years old. The data were collected by home interviews, stored using the Statistical Package for the Social Sciences (SPSS) software and presented in tables as absolute and relative frequency. The predominant ethnical group was Caucasian with an average age of 52 years. Among risk factors identified in the present study, an expressive number of overweight women were found. It is important and necessary to put in practice actions that identify the modifiable risk factors for the development of breast tumors in order to reduce morbidity and mortality levels due to this pathology.<br>Ese estudio pretendió identificar la prevalencia de los factores de riesgo para el cáncer de mama en mujeres de 40 la 69 años, en el municipio de Maringá. Fue realizado un estudio analítico, exploratorio, de corte transversal, tipo encuesta poblacional domiciliar. El estudio fue realizado con 439 mujeres entre 40 y 69 años. Los datos fueron recolectados durante entrevistas domiciliares, almacenados en el programa Statisitical Package for the Social Sciences (SPSS) y presentados en forma de frecuencia absoluta y relativa. La mayor parte de las mujeres estudiadas pertenecía a la raza blanca, con promedio de edad de 52 años. Entre los factores de riesgo identificados se destaca el gran número de mujeres con sobrepeso. Se resalta la importancia de la implementación de acciones que tengan por objetivo identificar los factores de riesgo modificables para el desarrollo del cáncer de mama para, con eso, disminuir el número de nuevos casos y de muerte por esa patología.<br>Esse estudo pretendeu identificar a prevalência dos fatores de risco para o câncer de mama em mulheres de 40 a 69 anos, no município de Maringá. Foi realizado estudo analítico, exploratório, de corte transversal, tipo inquérito populacional domiciliar. O estudo foi realizado com 439 mulheres entre 40 e 69 anos. Os dados foram coletados por entrevistas domiciliares, armazenados no programa Statisitical Package for the Social Sciences (SPSS) e apresentados em forma de frequência absoluta e relativa. A maior parte das mulheres estudadas pertence à raça branca, com média de idade de 52 anos. Dentre os fatores de risco identificados destaca-se o grande número de mulheres acima do peso. Ressalta-se a importância da implementação de ações que visem a identificação dos fatores de risco modificáveis para o desenvolvimento do câncer de mama para, com isso, diminuir o número de adoecimento e morte por essa patologia