Evaluation du stress oxydatif par le dosage de la vitamine C et de ses dérivés chez des patients hémodialysés chroniques

DIJON-BU Médecine Pharmacie (212312103) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF

Etude in vitro et in vivo de l'alloréactivité et du microchimérisme après injection de cellules dendritiques matures et d'un anticorps monoclonal anti-cd4 chez le rat

DIJON-BU Médecine Pharmacie (212312103) / SudocPARIS-BIUP (751062107) / SudocSudocFranceF

VALEUR DIAGNOSTIQUE ET PRONOSTIQUE DE LA TOMOSCINTIGRAPHIE MYOCARDIQUE DANS L'EVALUATION CORONAIRE AVANT TRANSPLANTATION RENALE

DIJON-BU Médecine Pharmacie (212312103) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF

Endothelial cells in organ transplantation: Friends or foes?

International audienceVascular endothelial cells are the first interface between donor and recipient in organ transplantation. Endothelial cells and smooth muscle cells are key actors of acute and chronic rejection processes in organ allografting, but they also have the capacity to protect themselves from allograft-induced injury. Recent advances in our understanding of the precise mechanisms leading to endothelial dysfunction or, on the contrary, to endothelial protection, suggest that therapeutic interventions targeting endothelial cells could improve allograft survival and have even raised the question of whether such manipulations can be considered with a view to inducing immunological tolerance

Diminution de la réponse alloréactive in vitro et in vivo par association de cellules dendritiques matures du donneur et d’un anticorps monoclonal anti-CD4 chez le Rat

Nous avons montré, dans un modèle histo-incom- patible Rat Lewis receveur-Rat Wistar-Furth donneur, que l’association cellules dendritiques matures du donneur-anticorps monoclonal anti-CD4 non déplétant diminue la réponse alloréactive, étudiée en cultures mixtes splénocytaires unidirectionnelles, d’une manière plus prononcée que l’anti-CD4 seul., aussi bien in vitro que 30 jours après injection au Rat Lewis. Ces résultats suggèrent qu’un anticorps monoclonal anti-CD4 peut orienter des cellules dendritiques matures, habituellement responsables de rejets aigus, vers un effet tolérogène par des mécanismes qui restent à déterminer

Successful hepatorenal transplantation in hereditary amyloidosis caused by a frame-shift mutation in fibrinogen Aalpha-chain gene.

International audienceHereditary systemic amyloidosis comprises several autosomal dominant diseases caused by mutations in a number of plasma proteins, including the fibrinogen Aalpha-chain. Four mutations in the fibrinogen Aalpha-chain that are able to induce amyloidosis have been identified so far, the most common being the Glu526Val mutation. We have observed a family in which the father and his son reached end-stage renal failure because of renal amyloidosis induced by a frame-shift mutation in the fibrinogen Aalpha-chain gene producing a novel amyloid protein. Two kidney transplantations in the father and one in the son resulted in fast graft loss caused by recurrence of amyloid deposition. We then performed hepatorenal transplantation in the son. Three years later, liver and kidney functions are normal without recurrence of amyloid deposition. This case, together with three others with the Glu526Val mutation in the extensive literature, suggests that liver transplantation can cure hereditary fibrinogen amyloidosis, whatever the mutation may be

Urinary cytotoxic molecular markers for a noninvasive diagnosis in acute renal transplant rejection.

International audiencePerforin (P), Granzyme B (GB) and Fas-Ligand (FAS-L) are cytotoxic molecules involved in acute rejection (AR) after renal transplantation. A noninvasive diagnostic test to monitor AR and other complications could improve clinical management. We investigated the predictive and diagnostic interest of target mRNA measurements, with a quantitative PCR assay, in AR, as well as in other clinical complications recurrent in kidney transplantation. One hundred and sixty-two urine specimens from 37 allograft recipients were investigated. Clinical settings were AR, urinary tract infection (UTI), cytomegalovirus infection (CMVi) or disease (CMVd), chronic allograft nephropathy (CAN), delayed graft function (DGF) and stable graft course (controls). In the case of AR, mRNA levels of all three molecules were significantly higher than in recipients not showing any clinically evident signs of complication. Indeed, it was observed that expression levels of P, GB and Fas-L mRNA also increase in other clinical situations such as UTI, CMV and DGF. Finally, kinetic studies in three patients with AR revealed that increased P, GB and Fas-L mRNA levels could precede or were concomitant with increased serum creatinin levels. P, GB and Fas-L gene expression in urine specimens were upregulated in AR episodes but also in UTI, CMV infection and DGF. Therefore, this technique would appear to be of limited clinical value as a noninvasive method of diagnosing AR