Net ecosystem CO₂ exchange measurements by the closed chamber method and the eddy covariance technique and their dependence on atmospheric conditions

Carbon dioxide flux measurements in ecosystem sciences are mostly conducted by eddy covariance technique or the closed chamber method. But there is a lack of detailed comparisons that assess present differences and uncertainties. To determine underlying processes, a 10-day, side-by-side measurement of the net ecosystem exchange with both techniques was evaluated with regard to various atmospheric conditions during the diurnal cycle. It was found that, depending on the particular atmospheric condition, the chamber carbon dioxide flux was either (i) equal to the carbon dioxide flux measured by the reference method eddy covariance, by day with well-developed atmospheric turbulence; (ii) higher, in the afternoon in times of oasis effect; (iii) lower, predominantly at night while large coherent structure fluxes or high wind velocities prevailed; or (iv) showed less variation in the flux pattern, at night while stable stratification was present. At night – when respiration forms the net ecosystem exchange – lower chamber carbon dioxide fluxes were found. In the afternoon – when the ecosystem is still a net carbon sink – the carbon dioxide fluxes measured by the chamber prevailed. These two complementary aspects resulted in an overestimation of the ecosystem sink capacity by the chamber of 40% in this study

The competitive NMDA antagonist CPP protects substantia nigra neurons from MPTP-induced degeneration in primates

Degeneration of nigrostriatal dopaminergic neurons is the primary histopathological feature of Parkinson's disease. The neurotoxin MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) induces a neurological syndrome in man and non-human primates very similar to idiopathic Parkinson's disease by selectively destroying dopaminergic nigrostriatal neurons. This gives rise to the hypothesis that Parkinson's disease may be caused by endogenous or environmental toxins. Endogenous excitatory amino acids (EAAs) such as L-glutamate could be involved in neurodegenerative disorders including Parkinson's disease. We report in this study that the competitive NMDA antagonist CPP (3-((+/-)-2-carboxypiperazin-4-yl)-propyl-1-phosphonic acid) protects nigral tyrosine hydroxylase (TH) positive neurons from degeneration induced by systemic treatment with MPTP in common marmosets. This indicates that EAAs are involved in the pathophysiological cascade of MPTP-induced neuronal cell death and that EAA antagonists may offer a neuroprotective therapy for Parkinson's disease

Dorsal Bone-Ligament-Bone Reconstruction of Chronic Lunotriquetral Instability: Biomechanical Testing.

Purpose Lunotriquetral (LT) instability is uncommon and few biomechanical analyses of the condition exist. For chronic LT instabilities, arthrodesis has long been the treatment of choice but has a high risk for nonunion. The aim of this study was to evaluate an alternative treatment option using a bone-ligament-bone graft in a cadaver model and compare it with a conventional arthrodesis. Methods We used 10 cadaveric forearms with different loading positions. We employed computed tomography scans to evaluate the LT joint. Scans were performed with the joint intact after we sectioned the dorsal LT ligament and the palmar LT ligament. The joints were then reconstructed using a bone-ligament-bone graft from the capitate-hamate joint as well as with a compression screw simulating arthrodesis. The joints were then rescanned and 3-dimensional analysis was performed using specialized 3-dimensional software. Results Sectioning the dorsal part of LT ligament had little effect on kinematics; however, additional division of the palmar LT ligament resulted in increased mobility. Restoration of physiological kinematics could be partially achieved after bone-ligament-bone reconstruction. Arthrodesis showed increased intercarpal motion in the adjacent scapholunate and lunocapitate joints compared with the bone-ligament-bone reconstruction. Conclusions The bone-ligament-bone reconstruction displayed physiologic carpal kinematics in the adjacent joints compared with arthrodesis. It provided enough stability but still some mobility in the LT joint to be able to use it as a treatment modality for chronic LT instability without the risk for nonunion. Decreased intercarpal motion was not statistically significant although there appeared to be a trend toward it. Type of study/level of evidence Therapeutic IV

Transcriptional alterations under continuous or pulsatile dopaminergic treatment in dyskinetic rats

Continuous dopaminergic treatment is considered to prevent or delay the occurrence of dyskinesia in patients with Parkinson's disease (PD). Rotigotine is a non-ergolinic D3>D2>D1 dopamine-receptor agonist for the treatment of PD using a transdermal delivery system providing stable plasma levels. We aimed to investigate the differential influence on gene expression of pulsatile l-DOPA or rotigotine versus a continuous rotigotine treatment. The gene expression profile within the nigro-striatal system of unilateral 6-hydroxydopamine-lesioned rats was assessed in order to differentiate potential changes in gene expression following the various treatment using Affymetrix microarrays and quantitative RT-PCR. The expression of 15 genes in the substantia nigra and of 11 genes in the striatum was altered under pulsatile treatments inducing dyskinetic motor response, but was unchanged under continuous rotigotine treatment that did not cause dyskinetic motor response. The route of administration of a dopaminergic drug is important for the induction or prevention of motor abnormalities and adaptive gene expressions. The decline of neurotrophin-3 expression under pulsatile administration was considered of particular importanc

Multifunctional adaptive façade at iba 2013; design studies for an integral energy harvesting façade shading system

As part of the international exhibition ‘Bauausstellung’ IBA 2013 in Hamburg, Germany, architects from KVA MATx team and engineers from Knippers Helbig Advanced Engineering have developed an integral energy harvesting façade shading system for their ‘Softhouse’ project. Its overall concept includes an energy harvesting hybrid textile roof featuring flexible photovoltaics, which contributes to create a micro-climate for the building as a shading roof for the terrace and glass façade. This responsive façade is based on a textile hybrid system, using textile membranes and glass fibre reinforced plastics (GFRP) in an intricate form- and bending-active structure. This paper will discuss the multiple design studies that were undertaken to develop a system that satisfies the, at times, diametrically opposed demands from architecture, building physics, structural engineering and technical approval. Furthermore, detailed information will be given on the design specifications for using GFRP in bending-active elements and the Finite-Element simulation techniques used for the form-finding and structural analysis

Modeling sporadic alzheimer's disease: the insulin resistant brain state generates multiple long-term morphobiological abnormalities inclusive hyperphosphorylated tau protein and amyloid-beta. A Synthesis

Nosologically, Alzheimer's disease (AD) is not a single disorder. Missense gene mutations involved in increased formation of the amyloid-beta protein precursor derivatives amyloid-beta (Abeta)_{1-40} and Abeta_{1-42/43} lead to autosomal dominant familial AD, found in the minority of AD cases. However, millions of subjects suffer from sporadic AD (sAD) of late onset, for which no convincing evidence suggests Abeta as the primary disease-generating compound. Environmental factors operating during pregnancy and postnatally may affect susceptibility genes and stress factors (e.g., cortisol), consequently affecting brain development both structurally and functionally, causing disorders becoming manifest late in life. With aging, a desynchronization of biological systems may result, increasing further brain entropy/declining criticality. In sAD, this desynchronization may involve stress components, cortisol and noradrenaline, reactive oxygen species, and membrane damage as major candidates causing an insulin resistant brain state with decreased glucose/energy metabolism. This further leads to a derangement of ATP-dependent cellular and molecular work, of the cell function in general, as well as derangements in the endoplasmic reticulum/Golgi apparatus, axon, synapses, and membranes, in particular. A self-propagating process is thus generated, including the increased formation of hyperphosphorylated tau-protein and Abeta as abnormal terminal events in sAD rather than causing the disorder, as elaborated in the review

Zero modes of six-dimensional Abelian vortices

We analyze the fluctuations of Nielsen-Olesen vortices arising in the six-dimensional Abelian-Higgs model. The regular geometry generated by the defect breaks spontaneously six-dimensional Poincar\'e symmetry leading to a warped space-time with finite four-dimensional Planck mass. As a consequence, the zero mode of the spin two fluctuations of the geometry is always localized but the graviphoton fields, corresponding to spin one metric fluctuations, give rise to zero modes which are not localized either because of their behaviour at infinity or because of their behaviour near the core of the vortex. A similar situation occurs for spin zero fluctuations. Gauge field fluctuations exhibit a localized zero mode.Comment: 45 pages in Revtex style with 4 figure

Capturing the facets of evolvability in a mechanistic framework

‘Evolvability’ – the ability to undergo adaptive evolution – is a key concept for understanding and predicting the response of biological systems to environmental change. Evolvability has various facets and is applied in many ways, easily leading to misunderstandings among researchers. To clarify matters, we first categorize the mechanisms and organismal features underlying evolvability into determinants providing variation, determinants shaping the effect of variation on fitness, and determinants shaping the selection process. Second, we stress the importance of timescale when studying evolvability. Third, we distinguish between evolvability determinants with a broad and a narrow scope. Finally, we highlight two contrasting perspectives on evolvability: general evolvability and specific evolvability. We hope that this framework facilitates communication and guides future research