Repairing 2024 Aluminum Alloy via Electrospark Deposition Process: A Feasibility Study

The electrospark deposition (ESD) technique has been studied as a potential method to repair locally damaged 2024 rolled sheets supplied in natural-aged (T4) and artificial-aged (T6) conditions. The 2024-T4 and 2024-T6 tensile samples were first notched, and then the notches were filled (repaired) by ESD with the same aluminum alloy. The effect of process parameters on the microstructure of the filling material and the substrate properties was studied by optical and scanning electron microscopy. Tensile and hardness tests were performed. The tensile test showed that T4 and T6 as-repaired specimens had low tensile properties, which was due to defectiveness and residual stress caused by high cooling rate during reparation. However, the as-repaired specimens were heat-treated at either 135°C or 190°C to improve the mechanical properties. A better yield strength was observed for the T4 heat-treated alloy. The ductility and ultimate tensile strength did not change, being mainly affected by voids and microcracks

Corrosion Resistance Behaviour of recycled AlSi10Mg alloy: Surface Morphology and Acoustic Emission Investigation

The corrosion resistance behaviour of recycled AlSi10Mg alloy prepared using Selective Laser Melting (SLM) process is investigated. The specimens are exposed to salt solution attack (5% NaCl) atomized in uniform droplets, inside a controlled Salt Spray Test (SST) chamber for 1000 h. The surface morphology of the specimens exposed to different predefined exposure times (0 h, 6 h, 48 h, 168 h, 480 h and 1000 h) are investigated under Scanning Electron Microscope (SEM). The SEM micrographs shows the salient features of the corrosion attack such as the formation both of pits and corrosion products on samples surface in different exposure times in the SST chamber. Similarly, the Acoustic Emission (AE) signals generated during the corrosion process are recorded for the different exposure times. The AE waveforms are studied using advanced waveform processing techniques. The waveforms, in their time-frequency domain, provide detailed information on the characteristic features of the acoustic source. The different AE sources have been characterized from the time-frequency analysis of the waveforms

XAV939-mediated ARTD activity inhibition in human MB cell lines

Diphtheria toxin-like ADP-ribosyltransferases 1 and 5 (ARTD-1, ARTD-5) are poly ADP-ribose enzymes (PARP) involved in non-homologous end-joining (NHEJ), which is the major pathway of double-strand break (DSB) repair. In addition, ARTD-5, or Tankyrase (TNKS), is a positive regulator of the WNT signaling implicated in the development and biological behavior of many neoplasms, such as Medulloblastoma (MB), in which radiotherapy is an essential part of the treatment. The use of radiosensitizing agents may improve the therapeutic index in MB patients by increasing the efficacy of radiotherapy, while reducing toxicity to the neuroaxis. ARTD-5 seems to be a good molecular target for improving the current treatment of MB. In this study, we used the small molecule XAV939, a potent ARTD-5 inhibitor with a slight affinity for ARTD-1, in different human MB cell lines. XAV939 inhibited the WNT pathway and DNA-PKcs in our MB cells, with many biological consequences. The co-administration of XAV939 and ionizing radiations (IR) inhibited MB cells proliferation and clonogenic capacity, decreased their efficacy in repairing DNA damage, and increased IR-induced cell mortality. In conclusion, our in vitro data show that XAV939 could be a very promising small molecule in MB treatment, and these results lay the basis for further in vivo studies with the aim of improving the current therapy available for MB patients

Unconventional secretion of α-Crystallin B requires the Autophagic pathway and is controlled by phosphorylation of its serine 59 residue

α-Crystallin B (CRYAB or HspB5) is a chaperone member of the small heat-shock protein family that prevents aggregation of many cytosolic client proteins by means of its ATP-independent holdase activity. Surprisingly, several reports show that CRYAB exerts a protective role also extracellularly, and it has been recently demonstrated that CRYAB is secreted from human retinal pigment epithelial cells by an unconventional secretion pathway that involves multi-vesicular bodies. Here we show that autophagy is crucial for this unconventional secretion pathway and that phosphorylation at serine 59 residue regulates CRYAB secretion by inhibiting its recruitment to the autophagosomes. In addition, we found that autophagosomes containing CRYAB are not able to fuse with lysosomes. Therefore, CRYAB is capable to highjack and divert autophagosomes toward the exocytic pathway, inhibiting their canonical route leading to the lysosomal compartment. Potential implications of these findings in the context of disease-associated mutant proteins turn-over are discussed

Actin and microtubules differently contribute to vacuolar targeting specificity during the export from the er

Plants rely on both actin and microtubule cytoskeletons to fine-tune sorting and spatial targeting of membranes during cell growth and stress adaptation. Considerable advances have been made in recent years in the comprehension of the relationship between the trans-Golgi network/early endosome (TGN/EE) and cytoskeletons, but studies have mainly focused on the transport to and from the plasma membrane. We address here the relationship of the cytoskeleton with different endoplasmic reticulum (ER) export mechanisms toward vacuoles. These emergent features of the plant endomembrane traffic are explored with an in vivo approach, providing clues on the traffic regulation at different levels beyond known proteins’ functions and interactions. We show how traffic of vacuolar markers, characterized by different vacuolar sorting determinants, diverges at the export from the ER, clearly involving different components of the cytoskeleton

DIC analysis of mechanical response of tooth aligners under simulated swallowing acts

In this work, the mechanical and deformation behavior of clear Polyethylene Terephthalate-glycol (PET-g) aligners, under cyclic loading was investigated using a full-field optical technique: the Digital Image Correlation. In particular, the PET-g aligners thermoformed from 0.88 mm thick discs, were subjected to cyclic compression tests for 13000 load cycles from 0 to 50 N in the atmospheric environment (~25°C). This number of cycles was chosen because it simulates, on average, the intraoral load associated with the swallowing acts that an aligner is subjected to during the time of use of 1 week. At the same time, the results from the analysis of hysteresis loops obtained by the DIC technique were compared with those obtained by the testing machine. The mechanical response of clear aligners was evaluated in terms of maximum displacement, energy loss and relative stiffness along the load direction to seven different stages of the 13000 load cycles. A comparable trend was found between the measurements obtained by Digital Image Correlation analysis and the analysis of the hysteresis loops obtained from the cyclic compression tests. Furthermore, the morphological features of the PET-g aligner at the end of the tests were analyzed by optical microscopy (OM). The OM analyses showed that thesurface of PET-g aligner was affected by morphological variations such as high depressions and cracks

Cover crops and manure combined with commercial fertilizers differently affect yield and quality of processing tomato (Solanum lycopersicum l.) organically grown in puglia

Nitrogen is crucial for the productivity of agricultural systems, although there is a growing demand for alternative cultural practices that reduce the off-farm inputs of this fertilizer. Cover crops provide a suite of services; among these, they can affect soil nitrogen content. In addition, the use of manure can contribute to a decrease in nitrogen loads from external inputs. The aim of this study was to evaluate, in processing tomato grown under an organic system, the combined use of cover crops (i.e., Vicia faba var. minor, Triticum aestivum, and Raphanus sativus) or cattle manure, with reduced rates of commercial nitrogen fertilizers. The yield and some important qualitative characteristics of the tomato fruits were considered. V. faba was able to enhance the effects of the external input of nitrogen with respect to the yield. External inputs increased tocopherol content with respect to the control but only when it was integrated with manure. The content of carotenoids was not affected by commercial fertilizer alone; however, when it was supplied in addition to cattle manure or incorporation of R. sativus, a decrease in β-carotene was observed. Moreover, lycopene also decreased when T. aestivum or R. sativus were used as cover crops. We conclude that the sowing of nitrogen-fixing cover crops or the use of manure are good strategies for combining internal and external inputs for the sustainable production of processing tomato

Cultured myoblasts from patients affected by myotonic dystrophy type 2 exhibit senescence-related features: ultrastructural evidence

Myotonic dystrophy type 2 (DM2) is an autosomal dominant disorder caused by the expansion of the tetranucleotidic repeat (CCTG)n in the first intron of the Zinc Finger Protein-9 gene. In DM2 tissues, the expanded mutant transcripts accumulate in nuclear focal aggregates where splicing factors are sequestered, thus affecting mRNA processing. Interestingly, the ultrastructural alterations in the splicing machinery observed in the myonuclei of DM2 skeletal muscles are reminiscent of the nuclear changes occurring in age-related muscle atrophy. Here, we investigated in vitro structural and functional features of satellite cell-derived myoblasts from biceps brachii, in the attempt to investigate cell senescence indices in DM2 patients by ultrastructural cytochemistry. We observed that in satellite cell-derived DM2 myoblasts, cell-senescence alterations such as cytoplasmic vacuolization, reduction of the proteosynthetic apparatus, accumulation of heterochromatin and impairment of the pre-mRNA maturation pathways occur earlier than in myoblasts from healthy patients. These results, together with preliminary in vitro observations on the early onset of defective structural features in DM2 myoblast derived-myotubes, suggest that the regeneration capability of DM2 satellite cells may be impaired, thus contributing to the muscular dystrophy in DM2 patients

Overexpression of CUGBP1 in skeletal muscle from adult classic myotonic dystrophy type 1 but not from myotonic dystrophy type 2

Myotonic dystrophy type 1 (DM1) and type 2 (DM2) are progressive multisystemic disorders caused by similar mutations at two different genetic loci. The common key feature of DM pathogenesis is nuclear accumulation of mutant RNA which causes aberrant alternative splicing of specific pre-mRNAs by altering the functions of two RNA binding proteins, MBNL1 and CUGBP1. However, DM1 and DM2 show disease-specific features that make them clearly separate diseases suggesting that other cellular and molecular pathways may be involved. In this study we have analysed the histopathological, and biomolecular features of skeletal muscle biopsies from DM1 and DM2 patients in relation to presenting phenotypes to better define the molecular pathogenesis. Particularly, the expression of CUGBP1 protein has been examined to clarify if this factor may act as modifier of disease-specific manifestations in DM. The results indicate that the splicing and muscle pathological alterations observed are related to the clinical phenotype both in DM1 and in DM2 and that CUGBP1 seems to play a role in classic DM1 but not in DM2. In conclusion, our results indicate that multisystemic disease spectrum of DM pathologies may not be explained only by spliceopathy thus confirming that the molecular pathomechanism of DM is more complex than that actually suggested