Avaliação de serviços em saúde mental no Brasil: revisão sistemática da literatura

ResumoA avaliação em saúde mental é um mecanismo capaz de produzir informações que contribuam positivamente para a tomada de decisão na área. Logo, é preciso apropriar-se das discussões existentes, refletindo sobre desafios e possibilidades na produção de conhecimento neste campo. Realizou-se uma revisão sistemática da produção científica brasileira sobre avaliação de serviços em saúde mental, identificando e discutindo métodos, perspectivas avaliativas e resultados. A busca de artigos ocorreu nas bases de dados IBECS, Lilacs e Scielo, com recorte temporal da publicação da lei 10.216. Foram encontrados 35 artigos por meio dos descritores e critérios de inclusão e exclusão utilizados. A produção da área concentrou-se nas regiões Sul e Sudeste, com diversos âmbitos e participantes, visando contribuir para o aprimoramento de serviços e decisões na área. Destacam-se os avanços no cuidado, com tratamento humanizado, participativo e comunitário, mas carecendo de maiores investimentos, qualificação profissional e melhorias organizacionais. Postula-se maior integração entre pesquisas, com as avaliações ultrapassando aspectos estruturais e a comparação com modelos hospitalocêntricos

Reproductive cycle of Macrobrachium amazonicum females (Crustacea, Palaemonidae)

Macrobrachium amazonicum is considered a favorite Brazilian species of freshwater prawn for cultivation as a result of its quick development and because it is easy to maintain in captivity. The aim of this work is to describe the sexual cycle stages and determine maturation age of the female M. amazonicum, which was collected monthly from June, 2002 to May, 2003 in the Jaguaribe River, Itaiçaba, Ceará. A monthly sample of water was also collected to determine the following parameters: temperature, dissolved oxygen, pH and salinity. A monthly sample of females was selected among the individuals caught, to determine the total weight (W T), carapace length (L C) and abdomen+telson length (L A+T) and to register the number of non-ovigerous females (NOF) and ovigerous females (OF). Determining ovarian maturation stages of M. amazonicum was done in a laboratory by observing macroscopic characters such as coloring, size, location and appearance of ovarians examined by transparent carapace. The first maturation age was determined from the relative frequency of the total length (L T) of young and adult females. The environmental parameters of the Jaguaribe River did not hold any influence in the number of individuals collected. A total of 1,337 prawns were sampled, 513 males (38.4%) and 824 females (61.6%). The proportion between males and females in the studied population was of 1:1.6. Among the collected females, 492 (50.7%) did not carry eggs in their abdomens (NOF) and 332 (40.3%) carried eggs in their abdomens (OF). There was no record of intact females. Non-ovigerous females with mature ovaries were recorded throughout all the months of collection. The female ovaries were classified as immature (IM), rudimentary (RU), intermediary (IN) and mature (M). M. amazonicum females reach their first sexual maturity between 4.5 and 5.5 cm of total length

Regulation of oocyte-specific chromatin organisation during prophase I by the histone demethylase Kdm5/Lid and other proteins

In Drosophila oocytes, chromosomes undergo dynamic reorganisation during the prophase of the first meiotic division. This is essential to prepare chromatin for synapsis, recombination and consequent chromosome segregation. The progression of meiotic prophase I is well described, while the molecular mechanisms and regulation of these dramatic chromosomal reorganisations are not well understood. Histone modifying enzymes are major regulators of chromatin structure, however, our knowledge of their roles in meiotic prophase I is still limited. In this work, I investigated the role of the histone demethylase Kdm5/Lid, which removes one of the trimethyl groups at Lys4 of Histone 3 (H3K4me3). I showed that Kdm5/Lid is important for the assembly of the synaptonemal complex, pairing of homologous centromeres, and the karyosome formation. Additionally, Kdm5/Lid promotes crossing over and therefore ensures accurate chromosome segregation. Although loss of Kdm5/Lid dramatically increased the level of H3K4me3 in oocytes, catalytically inactive Kdm5/Lid rescued the above cytological defects. Thereby, I found that Kdm5/Lid regulates chromatin architecture in meiotic prophase I oocytes independently of its demethylase activity. To further identify the regulators of meiotic chromatin organisation during prophase I, I carried out a small-scale RNAi screen for karyosome defects. I found that depletion of ubiquitin ligase components, SkpA, Cul-3 and Ubc-6, disrupted the karyosome formation and the assembly of the synaptonemal complex. The success of the small-scale screen motivated me to initiate the genome-scale RNAi screen for karyosome defects. I found 40 new genes that, when depleted, strongly impaired karyosome morphology. Further studies are required to confirm and elucidate their role in chromatin organisation in oocytes. Overall, my findings have advanced our understanding of the regulation of chromatin reorganisation during oocyte development. Because of the conservation between Drosophila and human meiosis, this study provides novel insights into the regulation of meiotic progression in human oocytes