Optimal left ventricular lead position assessed with phase analysis on gated myocardial perfusion SPECT

The aim of the current study was to evaluate the relationship between the site of latest mechanical activation as assessed with gated myocardial perfusion SPECT (GMPS), left ventricular (LV) lead position and response to cardiac resynchronization therapy (CRT). The patient population consisted of consecutive patients with advanced heart failure in whom CRT was currently indicated. Before implantation, 2-D echocardiography and GMPS were performed. The echocardiography was performed to assess LV end-systolic volume (LVESV), LV end-diastolic volume (LVEDV) and LV ejection fraction (LVEF). The site of latest mechanical activation was assessed by phase analysis of GMPS studies and related to LV lead position on fluoroscopy. Echocardiography was repeated after 6 months of CRT. CRT response was defined as a decrease of a parts per thousand yen15% in LVESV. Enrolled in the study were 90 patients (72% men, 67 +/- 10 years) with advanced heart failure. In 52 patients (58%), the LV lead was positioned at the site of latest mechanical activation (concordant), and in 38 patients (42%) the LV lead was positioned outside the site of latest mechanical activation (discordant). CRT response was significantly more often documented in patients with a concordant LV lead position than in patients with a discordant LV lead position (79% vs. 26%, p < 0.01). After 6 months, patients with a concordant LV lead position showed significant improvement in LVEF, LVESV and LVEDV (p < 0.05), whereas patients with a discordant LV lead position showed no significant improvement in these variables. Patients with a concordant LV lead position showed significant improvement in LV volumes and LV systolic function, whereas patients with a discordant LV lead position showed no significant improvements.Cardiovascular Aspects of Radiolog

Circum-Mediterranean cultural heritage and medicial plant uses in traditional animal healthcare: a field survey in eight selected areas within the RUBIA project

During the years 2003¿2005, a comparative ethnobotanical field survey was conducted on remedies used in traditional animal healthcare in eight Mediterranean areas. The study sites were selected within the EU-funded RUBIA project, and were as follows: the upper Kelmend Province of Albania; the Capannori area in Eastern Tuscany and the Bagnocavallo area of Romagna, Italy; Cercle de Ouezanne, Morocco; Sierra de Aracena y Picos de Aroche Natural Park in the province of Huelva, Spain; the St. Catherine area of the Sinai Peninsula, Egypt; Eastern and Western Crete, Greece; the Paphos and Larnaca areas of Cyprus; and the Mitidja area of Algeria. One hundred and thirty-six veterinary preparations and 110 plant taxa were recorded in the survey, with Asteraceae and Lamiaceae being the most quoted botanical families. For certain plant species the survey uncovered veterinary phytotherapeutical indications that were very uncommon, and to our knowledge never recorded before. These include Anabasis articulata (Chenopodiaceae), Cardopatium corymbosum (Asteraceae), Lilium martagon (Liliaceae), Dorycnium rectum (Fabaceae), Oenanthe pimpinelloides (Apiaceae), Origanum floribundum (Lamiaceae), Tuberaria lignosa (Cistaceae), and Dittrichia graveolens (Asteraceae). These phytotherapeutical indications are briefly discussed in this report, taking into account modern phytopharmacology and phytochemistry. The percentage of overall botanical veterinary taxa recorded in all the study areas was extremely low (8%), however when all taxa belonging to the same botanical genus are considered, this portion increases to 17%. Nevertheless, very few plant uses were found to be part of a presumed "Mediterranean" cultural heritage in veterinary practices, which raises critical questions about the concept of Mediterraneanism in ethnobotany and suggests that further discussion is required. Nearly the half of the recorded veterinary plant uses for mammals uncovered in this survey have also been recorded in the same areas in human folk medicine, suggesting a strong link between human and veterinary medical practices, and perhaps also suggesting the adaptive origins of a few medical practices. Since most of the recorded data concern remedies for treating cattle, sheep, goats, and camels, it would be interesting to test a few of the recorded phytotherapeuticals in the future, to see if they are indeed able to improve animal healthcare in breeding environments, or to raise the quality of dairy and meat products in the absence of classical, industrial, veterinary pharmaceuticals

Isolation of chromosome-21-specific DNA probes and their use in the analysis of nondisjunction in Down syndrome

Thirteen single-copy, chromosome-21-specific DNA probes were isolated from a recombinant library made from flow-sorted chromosome 21 DNA and regionally mapped using a panel of somatic cell hybrids. Five probes mapped in the 21q21-q22.1 region, six to the 21q22.1-qter region, and one to each of the regions 21q22.1-q22.2 and 21q22.3. Two of these probes, one of which maps in the critical region for Down syndrome, have recently been shown to be expressed at high levels in Down syndrome brain tissue (Stefani et al. 1988). Following preliminary screening for restriction fragment lenght polymorphisms (RFLPs), five polymorphisms were discovered with four of the chromosome 21 DNA probes. A frequent Msp I polymorphism detected by one of the probes was used in conjunction with four previously described polymorphic chromosome 21 probes to analyse the origin of nondisjunction in 33 families with a child or fetus with trisomy 21. The parental origin of the additional chromosome 21 was determined in 12 cases: in 9 (75%) of these it was derived from the mother and in the other 3 cases (25%) it was of paternal origin. Cytogenetic analysis of Q-banding heteromorphisms was informative in three of five families tested, and in each case the RFLP results were confirmed. The meiotic stage of nondisjunction was defined with confidence in five families, the results being obtained with pericentromeric RFLP or cytogenetic markers. Recombination between two nondisjoined chromosomes was demonstrated in one family and is consistent with the view that a lack of recombination between chromosome 21 homologues or failure of their conjunction is not the invariable cause of trisomy 21.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/47624/1/439_2004_Article_BF00293885.pd

Pesticides in export and domestic agriculture: Reconsidering market orientation and pesticide use in Costa Rica

The environmental impact of agro-export production in developing countries remains an important research topic. The political economy-inspired literature on developing country agro-exports maintains that export crops are pesticide intensive - or, more generally, environmentally destructive - while local and national market crops are less pesticide intensive, or environmentally benign. If used to draw conclusions about the impact of national market versus export market expansion, this view has significant limitations, most importantly the comparison of high-commodity value export crops with low-commodity value crops for national market. To overcome this and other limitations of prior analyses, this paper addresses the question: how does market orientation influence pesticide intensity where the same crops are grown for both the national market and for export? Data from a survey of Costa Rican vegetable farmers are used to compare pesticide intensity of 27 vegetable crops, five of which are produced for both national and export markets. The general pattern that emerges is that national market vegetables are more pesticide intensive than export vegetables in the area. Yet, controlling more for the crop variable is important, and specific comparisons of the five vegetables grown for both markets - carrot, chayote, corn, green beans, and squash - illustrate that market orientation alone does not determine pesticide intensity, but that it is jointly influenced by regulatory risk, crop value, and pest susceptibility, among other factors. Continued attention to both political economy and ecological processes in "second nature" will allow political ecology to make important contributions to understanding pesticide problems and implementing agroecological solutions. © 2007 Elsevier Ltd. All rights reserved

Beyond the circle of poison: Significant shifts in the global pesticide complex, 1976-2008

Almost 30 years after its introduction, the "circle of poison" remains a common conceptualization of the global pesticide complex among scholars and especially in popular understanding. The circle of poison describes a situation in which, pesticides banned in industrialized countries continue to be manufactured there and exported to developing countries, are then used in developing countries almost entirely on export crops, and return to industrialized countries as pesticide residues on food. Using secondary data and a case study of pesticide use in Costa Rica, I review the applicability of the circle of poison conceptualization to the current global pesticide complex. I argue that (1) the circle of poison is no longer accurate due to important global changes in pesticide regulation, production, trade, sales, and use driven by a number of dynamic economic, social, and ecological processes; (2) using industrialized countries' pesticide regulations as proxies for safety should be replaced by multi-characteristic risk assessments; and (3) revisions of the circle of poison conceptualization should be updated because of export farmers' adoption of newer classes of pesticides. The paper concludes by offering a new characterization of the global pesticide complex vis-à-vis pesticide use in developing countries: pesticide divergence by market orientation. © 2008 Elsevier Ltd. All rights reserved

Toward an integrated understanding of pesticide use intensity in Costa Rican vegetable farming

Understanding the factors that influence the adoption of synthetic pesticides has to date overshadowed explanations of variation in pesticide intensity. I conducted a survey of vegetable farmers in Northern Cartago and the Ujarrás Valley, Costa Rica, in 2003-2004 with the goal of explaining differences in pesticide intensity with reference to socioeconomic, political economic, and agroecological characteristics and relationships. Using ordinary least squares regression models, this paper explores the factors that influence pesticide use intensity in potato and squash production. Results indicate that many variables strongly influence pesticide intensity, including variables related to the farmer, farm household, political-economic relationships, the biophysical environment, and agroecological relationships. Conclusions discuss the need for an integrated approach to adequately understand pesticide intensity and potential policy interventions including agroecological education and extension, increased enforcement of pesticide residue limits in the national market, and land reform to allow for longer fallow periods. © Springer Science+Business Media, LLC 2008

Overlap of US FDA residue tests and pesticides used on imported vegetables: Empirical findings and policy recommendations

In the United States, the Environmental Protection Agency (EPA) registers pesticides and sets crop-specific tolerances while the Food and Drug Administration (FDA) enforces EPA regulations by testing plant-based foods for pesticide residues. Pesticide treatment histories are almost always unknown, especially on imported produce, posing an empirical question: to what extent do FDA's residue testing methods used on imported produce correspond to the pesticides used on the crops? In this article I show that FDA residue testing would have missed residues of the majority of pesticides used on two crops exported to the US from Costa Rica in 2003, suggesting that FDA residue testing on imported produce is inadequate in its coverage. Policy recommendations discussed include better communication of US tolerances to exporters around the world; increased testing for pesticides, especially fungicides, that are currently not part of FDA's regular testing procedures; and the creation of price floors and fair trade relationships in the transnational vegetable market to support farmers' attempts to comply. © 2009 Elsevier Ltd. All rights reserved

Overlap of US FDA residue tests and pesticides used on imported vegetables: Empirical findings and policy recommendations

In the United States, the Environmental Protection Agency (EPA) registers pesticides and sets crop-specific tolerances while the Food and Drug Administration (FDA) enforces EPA regulations by testing plant-based foods for pesticide residues. Pesticide treatment histories are almost always unknown, especially on imported produce, posing an empirical question: to what extent do FDA's residue testing methods used on imported produce correspond to the pesticides used on the crops? In this article I show that FDA residue testing would have missed residues of the majority of pesticides used on two crops exported to the US from Costa Rica in 2003, suggesting that FDA residue testing on imported produce is inadequate in its coverage. Policy recommendations discussed include better communication of US tolerances to exporters around the world; increased testing for pesticides, especially fungicides, that are currently not part of FDA's regular testing procedures; and the creation of price floors and fair trade relationships in the transnational vegetable market to support farmers' attempts to comply. © 2009 Elsevier Ltd. All rights reserved