Normal growth and development in mice over-expressing the CCN family member WISP3

Loss-of-function mutations in the gene WISP3 cause the autosomal recessive human skeletal disease Progressive Pseudorheumatoid Dysplasia, whereas mice with knockout mutations of Wisp3 have no phenotype. The lack of a phenotype in the Wisp3 knockout mice has constrained studies of the protein’s in vivo function. Over-expression experiments in zebrafish indicated that WISP3 may function as a BMP and Wnt signaling modulator. To determine whether these biologic activities are retained in mice, we created two strains of transgenic mice that over-express WISP3 in a broad array of tissues. Despite strong and persistent protein over-expression, the transgenic mice remained phenotypically indistinguishable from their non-transgenic littermates. Surprisingly, WISP3 contained in conditioned medium recovered from transgenic mouse primary kidney cell cultures was able to bind BMP and to inhibit BMP signaling in vitro. Factors that account for the difference between the in vitro and in vivo activities of WISP3 remain unknown. At present, the mouse remains a challenging model organism in which to explore the biologic function of WISP3

P3HT-Based Solar Cells: Structural Properties and Photovoltaic Performance

Each year we are bombarded with B.Sc. and Ph.D. applications from students that want to improve the world. They have learned that their future depends on changing the type of fuel we use and that solar energy is our future. The hope and energy of these young people will transform future energy technologies, but it will not happen quickly. Organic photovoltaic devices are easy to sketch, but the materials, processing steps, and ways of measuring the properties of the materials are very complicated. It is not trivial to make a systematic measurement that will change the way other research groups think or practice. In approaching this chapter, we thought about what a new researcher would need to know about organic photovoltaic devices and materials in order to have a good start in the subject. Then, we simplified that to focus on what a new researcher would need to know about poly-3-hexylthiophene:phenyl-C61-butyric acid methyl ester blends (P3HT: PCBM) to make research progress with these materials. This chapter is by no means authoritative or a compendium of all things on P3HT:PCBM. We have selected to explain how the sample fabrication techniques lead to control of morphology and structural features and how these morphological features have specific optical and electronic consequences for organic photovoltaic device applications

Using Microtechnology to Quantify Torso Angle During Match-Play in Field Hockey.

Warman, GE, Cole, MH, Johnston, RD, Chalkley, D, and Pepping, GJ. Using microtechnology to quantify torso angle during match-play in field hockey. J Strength Cond Res 33(10): 2648-2654, 2019-Field hockey is played in a dynamic environment placing specific postural demands on athletes. Little research has been devoted to understanding the nature of a player's torso postures in field hockey match-play and its relationship with the perceptuomotor demands of the sport. We used commercially available microtechnology worn by 16 athletes during a 6-match national tournament to quantify torso flexion/extension angles. Orientation was derived using the inertial and magnetic sensors housed within global positioning system devices, assessing torso angle in the sagittal plane from 91 individual match files. The main independent variable was playing position, whereas the dependent variable was torso flexion/extension, presented as a percentage of playing time spent in 15 × 10° torso postural bands ranging from ≥40° extension to ≥90° flexion. It was shown that athletes spent 89.26% of their playing time in various torso postures, ranging from 20 to 90° of flexion. Defenders spent more time than midfielders (p = 0.004, effect size [ES] = 0.43) and strikers (p = 0.004; ES = 0.44) in the posture band of 10-20° torso flexion, whereas midfielders spent more time between 20 and 30° of torso flexion (p = 0.05; ES = 0.32) than strikers. Conversely, strikers spent more time between 30 and 40° of flexion than defenders (p < 0.001; ES = 0.74). These results reflect the sport-specific and role-specific torso angles adopted by field hockey athletes during match-play. Coaching staff can use these data to gain insight into the postural demands of their sport and inform the preparation of athletes for the perception-action demands of competition