Hydrothermal hydrolysis of starch with CO2 and detoxification of the hydrolysates with activated carbon for bio-hydrogen fermentation.

The imminent use of hydrogen as an energy vector establishes the need for sustainable production technologies based on renewable resources. Starch is an abundant renewable resource suitable for bio-hydrogen generation. It was hypothesised that starch hydrolysates from a large (250 mL) hydrothermal reactor could support bioH2 fermentation without inhibition by toxic byproducts.\ud \ud Starch was hydrolysed at high concentrations (40 200 g.L-1) in hot compressed water (HCW) with CO2 at 30 bar in a 250 mL reactor, the largest so far for polysaccharide hydrolysis, at 180 235 °C, 15 min. Hydrolysates were detoxified with activated carbon (AC) and tested in biohydrogen fermentations. The maximum yield of glucose was 548 g.kg starch 1 carbon at 200 °C. 5 hydroxymethyl furfural, the main fermentation inhibitor, was removed by AC to support 70% more hydrogen production than the untreated hydrolysates. The potential utilization of starch hydrolysates from HCW treatment for upscaled fermentations is promising

Off-target effects of bacillus Calmette–Guérin vaccination on immune responses to SARS-CoV-2: implications for protection against severe COVID-19

Background and objectives: Because of its beneficial off-target effects against non-mycobacterial infectious diseases, bacillus Calmette–Guérin (BCG) vaccination might be an accessible early intervention to boost protection against novel pathogens. Multiple epidemiological studies and randomised controlled trials (RCTs) are investigating the protective effect of BCG against coronavirus disease 2019 (COVID-19). Using samples from participants in a placebo-controlled RCT aiming to determine whether BCG vaccination reduces the incidence and severity of COVID-19, we investigated the immunomodulatory effects of BCG on in vitro immune responses to SARS-CoV-2. Methods: This study used peripheral blood taken from participants in the multicentre RCT and BCG vaccination to reduce the impact of COVID-19 on healthcare workers (BRACE trial). The whole blood taken from BRACE trial participants was stimulated with γ-irradiated SARS-CoV-2-infected or mock-infected Vero cell supernatant. Cytokine responses were measured by multiplex cytokine analysis, and single-cell immunophenotyping was made by flow cytometry. Results: BCG vaccination, but not placebo vaccination, reduced SARS-CoV-2-induced secretion of cytokines known to be associated with severe COVID-19, including IL-6, TNF-α and IL-10. In addition, BCG vaccination promoted an effector memory phenotype in both CD4+ and CD8+ T cells, and an activation of eosinophils in response to SARS-CoV-2. Conclusions: The immunomodulatory signature of BCG’s off-target effects on SARS-CoV-2 is consistent with a protective immune response against severe COVID-19

Rubella immunization in human immunodeficiency virus type 1-infected children

Background: HIV infection can have important although sometimes unexpected consequences, such as contributing to enlargement of the pool of rubella-susceptible children.Methods: At the Federal University of São Paulo, Brazil, we assessed response to rubella immunization at 15 months of age in 15 human immunodeficiency virus type 1 (HIV)-infected children, 20 seroreverted children (SR) and 18 healthy control children born to HIV-seronegative mothers (CON). Blood samples were collected before and 3 months after vaccination. All HIV-infected children had started highly active antiretroviral therapy during their first 6 months of life. Serum samples were tested with a rubella IgG enzyme-linked immunosorbent assay kit.Results: HIV children in immunologic categories 2/3 had lower rubella antibody titers (geometric mean, 33 IU/mL) than those from CON (125 IU/mL) and SR group (236 IU/mL) (Tukey, P = 0.01). Antibody values after vaccination were positively associated with CD4 T cell numbers and negatively associated with HIV viral load assessed immediately before vaccination. the percentage of children with protective antibodies after vaccination (above 10.0 IU/mL) was also significantly different among groups (Fisher's exact test, P = 0.013): CON, 94%; SR, 100%; HIV category 1, 100%; HIV category 2/3, 62%.Conclusions: HIV-infected children with a preserved immune system at measles-mumps-rubella immunization can have a good response to rubella vaccine. in contrast, those in more advanced categories for HIV infection respond poorly.Universidade Federal de São Paulo, Dept Pediat, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Pediat, São Paulo, BrazilWeb of Scienc

Supplementation of growing bulls grazing Panicum maximum cv. Coloniao increases average daily gain and does not impact subsequent performance in feedlot phase

ABSTRACT To evaluate the influence of supplementation strategies on the average daily gain (ADG) of cattle in a rotational grazing system of Guinea grass (Panicum maximum cv. Coloniao) pastures during the rainy season. It was further evaluated the residual effects in the feedlot finishing phase. Seventy-five 8 month-old crossbred bull calves averaging 200.1 ± 2.5 kg of body weight (BW) were stratified and grouped in three into 25 blocks according to BW, and then randomly assigned to one of three supplementation treatments: control (no supplement), energy supplement [65 g crude protein (CP)/kg dry matter (DM)] or protein supplement (200 g CP/kg DM) both fed as 6 g/kg BW. All animals composed a single herd and were separated daily according to treatment groups for supplementation. After the grazing phase, all animals were moved to a feedlot and received the same diet up until slaughter. Guinea grass was subjected to rotational stocking with pre- and post-grazing heights of 76 and 43 cm, respectively. Energy and protein supplements provided similar ADG (P > 0.05; 0.94 kg/day) which was greater than for non-supplemented animals during the grazing phase (P ≤ 0.05; 0.74 kg/day), but there was no difference in the subsequent feedlot phase (P > 0.05; 1.45 kg/day). The protein supplement had no incremental effects over the energy supplement on ADG and carcass traits of growing crossbreed bulls during the grazing phase, indicating that either source could be used, and that an energy supplement would lead to more efficient nutrient use coming from the grass

Supplementation of growing bulls grazing Panicum maximum cv. Coloniao increases average daily gain and does not impact subsequent performance in feedlot phase

ABSTRACT To evaluate the influence of supplementation strategies on the average daily gain (ADG) of cattle in a rotational grazing system of Guinea grass (Panicum maximum cv. Coloniao) pastures during the rainy season. It was further evaluated the residual effects in the feedlot finishing phase. Seventy-five 8 month-old crossbred bull calves averaging 200.1 ± 2.5 kg of body weight (BW) were stratified and grouped in three into 25 blocks according to BW, and then randomly assigned to one of three supplementation treatments: control (no supplement), energy supplement [65 g crude protein (CP)/kg dry matter (DM)] or protein supplement (200 g CP/kg DM) both fed as 6 g/kg BW. All animals composed a single herd and were separated daily according to treatment groups for supplementation. After the grazing phase, all animals were moved to a feedlot and received the same diet up until slaughter. Guinea grass was subjected to rotational stocking with pre- and post-grazing heights of 76 and 43 cm, respectively. Energy and protein supplements provided similar ADG (P > 0.05; 0.94 kg/day) which was greater than for non-supplemented animals during the grazing phase (P ≤ 0.05; 0.74 kg/day), but there was no difference in the subsequent feedlot phase (P > 0.05; 1.45 kg/day). The protein supplement had no incremental effects over the energy supplement on ADG and carcass traits of growing crossbreed bulls during the grazing phase, indicating that either source could be used, and that an energy supplement would lead to more efficient nutrient use coming from the grass

Dietary Patterns in Portuguese Children and Adolescent Population: The UPPER Project

Considering the nature, extent, and purpose of food processing, this study aims to identify dietary patterns (DPs) and their associations with sociodemographic factors and diet quality in Portuguese children and adolescents. Cross-sectional data were obtained from the National Food, Nutrition and Physical Activity Survey (20152016) of the Portuguese population. Dietary intake was obtained from two non-consecutive days and food items were classified according to the NOVA system. The proportion (in grams) of foods in the total daily diet was considered to identify DPs by latent class analysis, with age and sex as concomitant variables. Associations of DPs with sociodemographic characteristics were assessed using multinomial logistic regression. Linear regressions adjusted by sociodemographic characteristics tested associations of DPs with diet quality. DPs identified were: Unhealthy (higher sugar-sweetened beverages, industrial breads, and sausages intake), Traditional (higher vegetables, fish, olive oil, breads, ultra-processed yogurts, and sausages intake), and Dairy (higher intake of milk, yogurt, and milk-based beverages). Unhealthy was associated with older ages and lower intake of dietary fibre and vitamins and the highest free sugars and ultra-processed foods (UPF), although all DPs presented significant consumption of UPF. These findings should be considered for the design of food-based interventions and school-feeding policies in Portugal.</jats:p