The mitochondrial DNA 4,977-bp deletion and its implication in copy number alteration in colorectal cancer

<p>Abstract</p> <p>Background</p> <p>Qualitative and quantitative changes in human mitochondrial DNA (mtDNA) have been implicated in various cancer types. A 4,977 bp deletion in the major arch of the mitochondrial genome is one of the most common mutations associated with a variety of human diseases and aging.</p> <p>Methods</p> <p>We conducted a comprehensive study on clinical features and mtDNA of 104 colorectal cancer patients in the Wenzhou area of China. In particular, using a quantitative real time PCR method, we analyzed the 4,977 bp deletion and mtDNA content in tumor tissues and paired non-tumor areas from these patients.</p> <p>Results</p> <p>We found that the 4,977 bp deletion was more likely to be present in patients of younger age (≤65 years, p = 0.027). In patients with the 4,977 bp deletion, the deletion level decreased as the cancer stage advanced (p = 0.031). Moreover, mtDNA copy number in tumor tissues of patients with this deletion increased, both compared with that in adjacent non-tumor tissues and with in tumors of patients without the deletion. Such mtDNA content increase correlated with the levels of the 4,977 bp deletion and with cancer stage (p < 0.001).</p> <p>Conclusions</p> <p>Our study indicates that the mtDNA 4,977 bp deletion may play a role in the early stage of colorectal cancer, and it is also implicated in alteration of mtDNA content in cancer cells.</p

Psychometric properties of the Negative Stereotypes Towards Aging Questionnaire (CENVE) among a sample of Portuguese adults

Stereotyped beliefs about old age and the aging process have proven to strongly promote negative behaviors toward the elderly, with unfavorable influences on their mental and physical health. Therefore, it is important to assess negative aging attitudes with brief but reliable and validated measurement instruments. The psychometric properties of the Portuguese version of the Negative Stereotypes Towards Aging Questionnaire (15 items self-reported) are explored and described in a sample of 302 participants (213 females and 89 males) between the ages of 18 and 59 years old. Confirmatory Factor Analysis shows a one-factor structure, similar to the Spanish version. The internal reliability and mean inter-item correlation showed satisfactory psychometric proprieties. Factorial ANOVA reveals that differences in agism beliefs are mainly related to educational level, with lower negative stereotypes in more educated adults. No differences were found concerning gender. This instrument may be a useful tool to assess negative stereotypes toward old age and the aging process.info:eu-repo/semantics/publishedVersio

Testing a model of successful aging in a cohort of masters swimmers

Due to their high physical functioning, masters athletes are regularly proposed to exemplify successful aging. However, successful aging research on masters athletes has never been undertaken using a multidimensional successful aging model. To determine the best model for future successful aging research on masters athletes, we had masters swimmers (N = 169, M age = 57.4 years, 61% women) self-report subjective successful aging, and physical, psychological, cognitive, and social functioning. Using this data we tested one hypothesized and three alternative successful aging models. The hypothesized model fit the data best (-2LL = 2052.32, AIC = 1717) with physical (β = 0.31, SE = 0.11), psychological (β = 0.25, SE = 0.11), and social (β = 1.20, SE = 0.63) functioning factors significantly loading onto a higher order successful aging latent factor. Successful aging should be conceptualized as a multidimensional phenomenon in future masters athlete research. © 2018 Human Kinetics, Inc

Local hemostatic effect of cellulose tampons (Tampax) after stapled hemorrhoidopexy

once hemostasis is obtained after a stapled hemorrhoidectomy, standard cellulose rectal tampons left in place for 24 h absorb the local fluids, increase their volume and exert a local pressure on the stapled line without causing patient’s discomfort. This local hemostatic effect can be increased if the surface of the tampons is covered by a thin layer of fibrinogen and thrombi

Physical activity and the ageing body

Understanding experiences of physical activity (PA) as we age requires an engagement with the ageing body. The purpose of this chapter is to explore the complexities of negotiating PA as we physically age and to consider what are appropriate ways of putting the ageing body into movement. Firstly, we present a brief outline of the sociological understandings of the ageing body and locate PA within this. We then draw on empirical data from three research projects which explore PA in older people. Before promoting PA to older people, we must understand that becoming and remaining physically active is akin to a career and is a fragile endeavour, conditional on physical, cultural and phenomenological understandings of the body