119 research outputs found

    Spectral analysis of semi-actively controlled structures subjected to blast loading

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    This paper investigates the possibility of controlling the response of typical portal frame structures to blast loading using a combination of semi-active and passive control devices. A one storey reinforced concrete portal frame is modelled using non-linear finite elements with each column discretised into multiple elements to capture the higher frequency modes of column vibration response that are typical features of blast responses. The model structure is subjected to blast loads of varying duration, magnitude and shape, and the critical aspects of the response are investigated over a range of structural periods in the form of blast load response spectra. It is found that the shape or length of the blast load is not a factor in the response, as long as the period is less than 25% of the fundamental structural period. Thus, blast load response can be expressed strictly as a function of the momentum applied to the structure by a blast load. The optimal device arrangements are found to be those that reduce the first peak of the structural displacement and also reduce the subsequent free vibration of the structure. Semi-active devices that do not increase base shear demands on the foundations in combination with a passive yielding tendon are found to provide the most effective control, particularly if base shear demand is an important consideration, as with older structures. The overall results are summarised as response spectra for eventual potential use within standard structural design paradigms

    A simple boiling-based DNA extraction for RAPD profiling of landfarm soil to provide representative metagenomic content

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    Landfarm soils are employed in industrial and petrochemical residue bioremediation. This process induces selective pressure directed towards microorganisms capable of degrading toxic compounds. Detailed description of taxa in these environments is difficult due to a lack of knowledge of culture conditions required for unknown microorganisms. A metagenomic approach permits identification of organisms without the need for culture. However, a DNA extraction step is first required, which can bias taxonomic representativeness and interfere with cloning steps by extracting interference substances. We developed a simplified DNA extraction procedure coupled with metagenomic DNA amplification in an effort to overcome these limitations. The amplified sequences were used to generate a metagenomic data set and the taxonomic and functional representativeness were evaluated in comparison with a data set built with DNA extracted by conventional methods. The simplified and optimized method of RAPD to access metagenomic information provides better representativeness of the taxonomical and metabolic aspects of the environmental samples.Conselho Nacional de Pesquisa e Desenvolvimento (CNPq) [558272/2009-6]Conselho Nacional de Pesquisa e Desenvolvimento (CNPq)Fundacao de Amparo a Pesquisa do Estado da Bahia (FAPESB)Fundacao de Amparo a Pesquisa do Estado da Bahia (FAPESB

    Measuring the depth of invasion in vulvar squamous cell carcinoma: interobserver agreement and pitfalls

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    Aims: The depth of invasion is an important prognostic factor for patients with vulvar squamous cell carcinoma (SCC). The threshold of 1 mm distinguishes between FIGO stages IA and ≥IB disease and guides the need for groin surgery. Therefore, high interobserver agreement is crucial. The conventional and the alternative method are described to measure the depth of invasion. The aims of this study were to assess interobserver agreement for classifying the depth of invasion using both methods and to identify pitfalls. Methods and results: Fifty slides of vulvar SCC with a depth of invasion approximately 1 mm were selected, digitally scanned and independently assessed by 10 pathologists working in a referral or oncology centre and four pathologists in training. The depth of invasion was measured using both the conventional and alternative method in each slide and categorised into ≤1 and >1 mm. The percentage of agreement and Light’s kappa for multi-rater agreement were calculated, and 95% confidence intervals were calculated by bootstrapping (1000 runs). The agreement using the conventional method was moderate (κ = 0.57, 95% confidence interval = 0.45–0.68). The percentage of agreement among the participating pathologists using the conventional method was 85.0% versus 89.4% using the alternative method. Six pitfalls were identified: disagreement concerning which invasive nest is deepest, recognition of invasive growth and where it starts, curved surface, carcinoma situated on the edge of the tis

    Galaxy bulges and their massive black holes: a review

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    With references to both key and oft-forgotten pioneering works, this article starts by presenting a review into how we came to believe in the existence of massive black holes at the centres of galaxies. It then presents the historical development of the near-linear (black hole)-(host spheroid) mass relation, before explaining why this has recently been dramatically revised. Past disagreement over the slope of the (black hole)-(velocity dispersion) relation is also explained, and the discovery of sub-structure within the (black hole)-(velocity dispersion) diagram is discussed. As the search for the fundamental connection between massive black holes and their host galaxies continues, the competing array of additional black hole mass scaling relations for samples of predominantly inactive galaxies are presented.Comment: Invited (15 Feb. 2014) review article (submitted 16 Nov. 2014). 590 references, 9 figures, 25 pages in emulateApJ format. To appear in "Galactic Bulges", E. Laurikainen, R.F. Peletier, and D.A. Gadotti (eds.), Springer Publishin
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