23 research outputs found
Vascularização arterial da região do nó sinoatrial em corações suÃnos: origem, distribuição e quantificação
‘Candidatus Phytoplasma asteris’, a novel phytoplasma taxon associated with aster yellows and related diseases.
Aster yellows (AY) group (16SrI) phytoplasmas are associated with over 100 economically
important diseases worldwide and represent the most diverse and widespread phytoplasma
group. Strains that belong to the AY group form a phylogenetically discrete subclade within the
phytoplasma clade and are related most closely to the stolbur phytoplasma subclade, based on
analysis of 16S rRNA gene sequences. AY subclade strains are related more closely to their
culturable relatives, Acholeplasma spp., than any other phytoplasmas known. Within the AY
subclade, six distinct phylogenetic lineages were revealed. Congruent phylogenies obtained by
analyses of tuf gene and ribosomal protein (rp) operon gene sequences further resolved the
diversity among AY group phytoplasmas. Distinct phylogenetic lineages were identified by RFLP
analysis of 16S rRNA, tuf or rp gene sequences. Ten subgroups were differentiated, based on
analysis of rp gene sequences. It is proposed that AY group phytoplasmas represent at least one
novel taxon. Strain OAY, which is a member of subgroups 16SrI-B, rpI-B and tufI-B and is
associated with evening primrose (Oenothera hookeri ) virescence in Michigan, USA, was selected
as the reference strain for the novel taxon ‘Candidatus Phytoplasma asteris’. A comprehensive
database of diverse AY phytoplasma strains and their geographical distribution is presented
Identidade molecular dos fitoplasmas associados aos enfezamentos do tomateiro e da berinjela com base na análise do gene 16S rDNA
‘Candidatus Phytoplasma’, a taxon for the wall-less non-helical prokaryotes that colonize plant phloem and insects.
'Candidatus Phytoplasma', a taxon for the wall-less, non-helical prokaryotes that colonize plant phloem and insects
The trivial name 'phytoplasma' has been adopted to collectively name wall-less, non-helical prokaryotes that colonize plant phloem and insects, which were formerly known as mycoplasma-like organisms. Although phytoplasmas have not yet been cultivated in vitro, phylogenetic analyses based on various conserved genes have shown that they represent a distinct, monophyletic clade within the class Mollicutes. It is proposed here to accommodate phytoplasmas within the novel genus 'Candidatus (Ca.) Phytoplasma'. Given the diversity within 'Ca. Phytoplasma', several subtaxa are needed to accommodate organisms that share < 97.5% similarity among their 16S rRNA gene sequences. This report describes the properties of 'Ca. Phytoplasma', a taxon that includes the species 'Ca. Phytoplasma aurantifolia' (the prokaryote associated with witches'-broom disease of small-fruited acid lime), 'Ca. Phytoplasma australiense' (associated with Australian grapevine yellows), 'Ca. Phytoplasma fraxini' (associated with ash yellows), 'Ca. Phytoplasma japonicum' (associated with Japanese hydrangea phyllody), 'Ca. Phytoplasma brasiliense' (associated with hibiscus witches'-broom in Brazil), 'Ca. Phytoplasma castaneae' (associated with chestnut witches'-broom in Korea), 'Ca. Phytoplasma asteris' (associated with aster yellows), 'Ca. Phytoplasma mali' (associated with apple proliferation), 'Ca. Phytoplasma phoenicium' (associated with almond lethal disease), 'Ca. Phytoplasma trifolii' (associated with clover proliferation), 'Ca. Phytoplasma cynodontis' (associated with Bermuda grass white leaf), 'Ca. Phytoplasma ziziphi' (associated with jujube witches'-broom), 'Ca. Phytoplasma oryzae' (associated with rice yellow dwarf) and six species-level taxa for which the Candidatus species designation has not yet been formally proposed (for the phytoplasmas associated with X-disease of peach, grapevine flavescence dor\ue9e, Central American coconut lethal yellows, Tanzanian lethal decline of coconut, Nigerian lethal decline of coconut and loofah witches'-broom, respectively). Additional species are needed to accommodate organisms that, despite their 16S rRNA gene sequence being > 97.5% similar to those of other 'Ca. Phytoplasma' species, are characterized by distinctive biological, phytopathological and genetic properties. These include 'Ca. Phytoplasma pyri' (associated with pear decline), 'Ca. Phytoplasma prunorum' (associated with European stone fruit yellows), 'Ca. Phytoplasma spartii' (associated with spartium witches'-broom), 'Ca. Phytoplasma rhamni' (associated with buckthorn witches'-broom), 'Ca. Phytoplasma allocasuarinae' (associated with allocasuarina yellows, 'Ca. Phytoplasma ulmi' (associated with elm yellows) and an additional taxon for the stolbur phytoplasma. Conversely, some organisms, despite their 16S rRNA gene sequence being < 97.5% similar to that of any other 'Ca. Phytoplasma' species, are not presently described as Candidatus species, due to their poor overall characterization. \ua9 2004 IUMS