Quality of refrigerated raw milk from buffalo cows (Bubalus bubalis bubalis) in different farms and seasons in Brazil

The present research aimed to examine the chemical and microbiological characteristics of refrigerated raw milk collected from buffalo-only dairy herds from different farms and seasons. Samples were collected from twelve farms producing buffalo milk, located in the Mideast region of São Paulo state, in Brazil, once a month throughout the dairy calendar year from 2011 to 2014. The analysis of fat, protein, lactose, dry extract, and total solids contents were carried out using Infrared Absorption. Residues for antimicrobial analysis were carried out using Delvo Test Kit technique, and both somatic cell count and standard plate count analysis were carried out using Flow Cytometry. The analysis of the chemical components as well as for the microbiological analysis of SPC proved to be statistically significant throughout the period. No antibiotic residues were found in the totality of the sample, hence indicating that the farmers complied with the current sanitary legislation in that country, as well as SCC; however, higher values were found for the SPC. Significant results for all parameters analyzed were found among the farms, indicating that price incentives paid for the quality of raw milk could be an alternative to dairy processing companies, favoring yield and quality of the final product

Short-range interactions in a two-electron system: energy levels and magnetic properties

The problem of two electrons in a square billiard interacting via a finite-range repulsive Yukawa potential and subjected to a constant magnetic field is considered. We compute the energy spectrum for both singlet and triplet states, and for all symmetry classes, as a function of the strength and range of the interaction and of the magnetic field. We show that the short-range nature of the potential suppresses the formation of ``Wigner molecule'' states for the ground state, even in the strong interaction limit. The magnetic susceptibility $\chi(B)$ shows low-temperature paramagnetic peaks due to exchange induced singlet-triplet oscillations. The position, number and intensity of these peaks depend on the range and strength of the interaction. The contribution of the interaction to the susceptibility displays paramagnetic and diamagnetic phases as a function of $T$.Comment: 12 pages,6 figures; to appear in Phys. Rev.

Calcium chloride toxicology for food safety assessment using zebrafish (Danio rerio) embryos

The salt calcium chloride (CaCl2) is widely used in industry as a food additive; levels for human consumption are regulated by international or governmental agencies. Generally, the food industry relies on toxicity studies conducted in mammals such as mice, rats, and rabbits for determining food safety. However, testing in mammals is time-consuming and expensive. Zebrafish have been used in a range of toxicological analyses and offer advantages with regard to sensitivity, time, and cost. However, information in not available with regard to whether the sensitivity of zebrafish to CaCl2 is comparable to the concentrations of CaCl2 used as food additives. The aim of this study was to compare the CaCl2 tolerance of zebrafish embryos and larvae with concentrations currently approved as food additives. Acute toxicity, embryotoxicity, cardiotoxicity, and neurotoxicity assays were used to determine the threshold toxic concentration of CaCl2 in zebrafish embryos and larvae. The data showed that doses above 0.4% had toxic effects on development and on the activity of the cardiac and neuronal systems. Furthermore, all embryos exposed to 0.8 and 1.6% of CaCl2 died after 24 hpf. These findings are consistent with the limits of CaCl2 concentrations approved by Codex Alimentarius. Therefore, zebrafish embryos could be suitable for screening food additives

Zebrafish as an alternative animal model in human and animal vaccination research

Much of medical research relies on animal models to deepen knowledge of the causes of animal and human diseases, as well as to enable the development of innovative therapies. Despite rodents being the most widely used research model worldwide, in recent decades, the use of the zebrafish (Danio rerio) model has exponentially been adopted among the scientific community. This is because such a small tropical freshwater teleost fish has crucial genetic, anatomical and physiological homology with mammals. Therefore, zebrafish constitutes an excellent experimental model for behavioral, genetic and toxicological studies which unravels the mechanism of various human diseases. Furthermore, it serves well to test new therapeutic agents, such as the safety of new vaccines. The aim of this review was to provide a systematic literature review on the most recent studies carried out on the topic. It presents numerous advantages of this type of animal model in tests of efficacy and safety of both animal and human vaccines, thus highlighting gains in time and cost reduction of research and analyzes

Zebrafish as an animal model for food safety research: trends in the animal research

Toxicity studies in mammals continue to be the most appropriate model for predicting risk in 5 humans, but they tend to be expensive and time-consuming. In the aftermath of the genetic 6 sequencing of zebrafish (Danio rerio), that species showed to be highly genetically homologous 7 to humans. The use of the zebrafish model to assess food toxicity is already a reality as it is 8 capable of biological processes difficult to reproduce in vitro. Studies of complex mechanisms 9 of absorption, distribution, metabolism and excretion as well as cellular and tissue interactions 10 are of great information value resulting in time, space and cost savings, when compared to 11 studies with rodents. This review addresses the relevance of zebrafish model in food safety 12 research, both in the use of ingredients and innocuous food additives as well as for establishing 13 levels of safe food contaminant residues present in the environment. Toxicological screening 14 using the zebrafish model integrate the evaluation of teratogenicity, cardiotoxicity, 15 hepatotoxicity, genotoxicity, neurotoxicity, endocrinetoxicity, reproductive and behavioral 16 aspects. These are important endpoints for food safety assessment, which take substantially less 17 time than in mammalian tests. Furthermore, it serves well as a screening test follow-up for 18 validating favorable results in murine models, hence accelerating the risk assessment process 19 of products submitted for approval and registration, prioritizing safe compounds and reducing 20 unnecessary costs in subsequent mammalian studies. In conclusion, zebrafish model can be a 21 useful tool for food safety tests, however, additional studies are needed to further validate this 22 model for registration of new food ingredients and additives

Zebrafish toxicological screening could aid Leishmaniosis drug discovery

Background Recently a screen from a library of 1.8 million compounds identified in vitro a potent activity of the 2-aminobenzimidazoles series against Leishmania infantum, the etiological agent responsible by over 20.000 deaths each year. Several analogs were synthesized and in vitro tested through an optimization program, leading to a promising 2-aminobenzimidazoles derived compound (2amnbzl-d) that was progressed to in vivo mice studies. However, the not expected toxic effects prevented its progression to more advanced preclinical and clinical phases of drug development. Due to limitations of cell models in detecting whole organism complex interactions, 90% of the compounds submitted to pre-clinical tests are reproved. The use of Zebrafish embryo models could improve this rate, saving mammals, time and costs in the development of new drugs. To test this hypothesis, we compared 2amnbzl-d with two compounds with already established safety profile: carbamazepine and benznidazole, using an embryo Zebrafish platform based on acute toxicity, hepatotoxicity, neurotoxicity and cardiotoxicity assays (Pltf-AcHpNrCd). Results Tests were performed blindly, and the results demonstrated the presence of lethal and teratogenic effects (CL50%: 14.8 µM; EC50%: 8.6 µM), hepatotoxic in concentrations above 7.5 µM and neurotoxic in embryos exposed to 15 µM of 2amnbzl-d. Nevertheless, benznidazole exposition showed no toxicity and only the 100 µM of carbamazepine induced a bradycardia. Conclusions Results using Pltf-AcHpNrCd with zebrafish reproduced that found in the toxicological tests with mammals to a portion of the costs and time of experimentation

Desempenho da alface em cultivo orgânico com e sem cobertura morta e diferentes lâminas d'água.

O experimento foi conduzido no período de 27/05/2006 a 02/08/2006 no SIPA (Sistema Integrado de Produção Agroecológica), localizado no município de Seropédica-RJ. Nesse trabalho, objetivou-se avaliar o desempenho da cultura da alface (Lactuca sativa L.)cultivada sob diferentes lâminas de irrigação em um solo sem e com cobertura de palhada da leguminosa gliricídia. Foram conduzidos dois experimentos simultâneos, utilizando o delineamento experimental de blocos ao acaso, em ambos os experimentos, nos quais foram aplicadas 5 lâminas de irrigação, correspondendo a 25, 50, 80, 100, 115 % da evapotranspiração da cultura (ETc), sendo a produção final avaliada por meio da determinação da massa fresca, área foliar e número de folhas. Até o nível de 100% ETc, todas as variáveis analisadas tiveram seus valores aumentados, e para o nível de 115%, houve um decrescimento das mesmas. Na produção de massa fresca total, o sistema de cultivo com utilização de cobertura morta foi superior ao sem cobertura não diferenciando estatisticamente ao nível de 5 % probabilidade pelo teste F somente nas lâminas de 25 e 115% ETc

Leading Charm Production in the Interacting Gluon Model

We discuss leading charm production in connection with energy deposition in the central rapidity region. Special attention is given to the correlation between production in central and fragmentation regions. If the fraction of the reaction energy released in the central region increases the asymmetry in the $x_F$ distributions of charmed mesons will become smaller. We illustrate this quantitatively with simple calculations performed using the Interacting Gluon Model. Leading beauty production is also considered.Comment: 13 pages in Latex file and 7 uuencoded ps-figure

Produtividade de raízes de mandioca consorciada com milho e caupi em sistema orgânico.

Foram avaliados sistemas orgânicos de produção de mandioca “de mesa”, em Seropédica (RJ). O experimento constou dos seguintes tratamentos: monocultivo de mandioca (cv. IAC 576-70) e consórcios com milho experimental (cv. Eldorado), caupi (cv. Mauá) e milho+caupi. O manejo orgânico foi padronizado e toda a área experimental irrigada durante o período de permanência do milho no sistema. Do milho, foram colhidas espigas verdes (imaturas) e a parte aérea acamada na superfície do solo. O caupi foi incluído como adubo verde e cortado na floração, sendo mantidos os resíduos na superfície do solo. Ambos os consortes ocuparam as entrelinhas da mandioca, de modo alternado, semeados após a primeira capina da cultura principal. A cultivar IAC 576-70 mostrou-se adaptada ao manejo orgânico, com produtividade de raízes de padrão comercial próxima a 31 Mg ha-¹. Não houve diferenças significativas entre o monocultivo e os três tipos de consórcios testados. A inclusão do milho representou potencial de renda adicional ao produtor, colhendo-se,em média, 18.125 espigas ha-¹, o que correspondeu a 5,1 Mg ha-¹. Os resíduos provenientes da roçada do caupi proporcionaram um aporte de biomassa fresca de 12 Mg ha-¹, com uma expressiva contribuição em nitrogênio (cerca de 44 kg de N ha-1). A fabácea leguminosa cobriu por completo as entrelinhas da mandioca,demonstrando seu potencial de controle à erosão e a ervas espontâneas. O consórcio triplo mostrou-se vantajoso tendo em vista que a receita obtida com a venda do milho verde justificaria os custos da irrigação, além dos benefícios da inclusão do caupi e da não interferência dos consortes na produtividade da mandioca