Orbital Parameter Determination for Wide Stellar Binary Systems in the Age of Gaia

The orbits of binary stars and planets, particularly eccentricities and inclinations, encode the angular momentum within these systems. Within stellar multiple systems, the magnitude and (mis)alignment of angular momentum vectors among stars, disks, and planets probes the complex dynamical processes guiding their formation and evolution. The accuracy of the \textit{Gaia} catalog can be exploited to enable comparison of binary orbits with known planet or disk inclinations without costly long-term astrometric campaigns. We show that \textit{Gaia} astrometry can place meaningful limits on orbital elements in cases with reliable astrometry, and discuss metrics for assessing the reliability of \textit{Gaia} DR2 solutions for orbit fitting. We demonstrate our method by determining orbital elements for three systems (DS Tuc AB, GK/GI Tau, and Kepler-25/KOI-1803) using \textit{Gaia} astrometry alone. We show that DS Tuc AB's orbit is nearly aligned with the orbit of DS Tuc Ab, GK/GI Tau's orbit might be misaligned with their respective protoplanetary disks, and the Kepler-25/KOI-1803 orbit is not aligned with either component's transiting planetary system. We also demonstrate cases where \textit{Gaia} astrometry alone fails to provide useful constraints on orbital elements. To enable broader application of this technique, we introduce the python tool \texttt{lofti\_gaiaDR2} to allow users to easily determine orbital element posteriors.Comment: 18 pages, 10 figures, accepted for publication in Ap

Impact Mediated Loading Cytoplasmic Loading of Macromolecules into Adherent Cells

The advent of modern molecular biology, including the development of gene array technologies, has resulted in an explosion of information concerning the specific genes activated during normal cellular development, as well as those associated with a variety of pathological conditions. These techniques have served as a highly efficient, broacI.-based screening approach for those specific genes involved. in regulating normal cellular physiology and identifying candidate genes directly associated with the etiology of specific disease states. However, this approach provides information at the transcriptional' level only and does not necessarily indicate . that the gene in question is in fact translated i~to a protein, or whether or not post-translational modification of the protein occurs. The critical importance of post-translational modification (i.e. phosphorylation, glycosylation, sialyation, etc.) to protein function has been recognized with regard to a number of proteins involved in a variety of important disease states. For example, altered glycosylation of beta-amyloid precursor protein results in an increase in the amount of beta-amyloid peptide generated and hence secreted as insoluble extracellular amyloid deposits (Georgopoulou, McLaughlin et al. 2001; Walter, Fluhrer et al. 2001), a pathological hal1~nark of Alzheimer's disease. Abnormal phosphoryla~ion of synapsin I has been linked to alterations in synaptic vesicle trafficking leading to defective neurotransmission in Huntington's disease (Lievens, Woodman et al. 2002). Altered phosphorylation of the TAU protein involved in microtubule function has been linked to a number of neurodegenative diseases such as Alzheimer's disease (Billingsley and Kincaid 1997; Sanchez, Alvarez-T~llada et a1. 2001). Aberrant siaIyation of cell/I surface antigens has been detected in a number of different tumor cell types and has been linked to the acquisition of a neoplastic phenotype (Sell 1990), while improper' sia1yation of sodium channels in cardiac tissue has been linked to heart failure (Ufret-Vincenty, Baro et al. 2001; Fozzard and Kyle 2002)

Differential expression of exosomal microRNAs in prefrontal cortices of schizophrenia and bipolar disorder patients

Exosomes are cellular secretory vesicles containing microRNAs (miRNAs). Once secreted, exosomes are able to attach to recipient cells and release miRNAs potentially modulating the function of the recipient cell. We hypothesized that exosomal miRNA expression in brains of patients diagnosed with schizophrenia (SZ) and bipolar disorder (BD) might differ from controls, reflecting either disease-specific or common aberrations in SZ and BD patients. The sources of the analyzed samples included McLean 66 Cohort Collection (Harvard Brain Tissue Resource Center), BrainNet Europe II (BNE, a consortium of 18 brain banks across Europe) and Boston Medical Center (BMC). Exosomal miRNAs from frozen postmortem prefrontal cortices with well-preserved RNA were isolated and submitted to profiling by Luminex FLEXMAP 3D microfluidic device. Multiple statistical analyses of microarray data suggested that certain exosomal miRNAs were differentially expressed in SZ and BD subjects in comparison to controls. RT-PCR validation confirmed that two miRNAs, miR-497 in SZ samples and miR-29c in BD samples, have significantly increased expression when compared to control samples. These results warrant future studies to evaluate the potential of exosome-derived miRNAs to serve as biomarkers of SZ and BD

Application of weight-height ratios and body indices to juvenile populations--the national health examination survey data

Properties of four weight-height ratios were studed in data from the U.S. Health Examination Survey (Cycles II and III) of a national probability sample of youths aged 6-17 yr (N = 13,867). These ratios (weight/height, weight/height2, weight/height3, weight/body surface area) were examined for their correlation with adiposity (infrascapular skinfold thickness) and muscle mass (estimated muscle circumference) and for their relationship to selected physiologic and biochemical measurements. Weight/height2 (Quetelet's index) correlates best with skinfold thickness for all age-race-sex groups. However, weight-height ratios may be a better indicator of muscle mass than of adiposity because the ratios generally show higher correlations with muscle circumference than with skinfold thickness. Weight/body surface area (BSA index) is the ratio which shows the highest overall correlation with muscle circumference. The relationships of the ratios are different for various biochemical and physiologic parameters, and these data can be used for selection of an index appropriate to the investigative aims of the study.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/22752/1/0000307.pd

Demonstration of an automated CFD system for three-dimensional flow simulations

In this paper the capabilities of an automated CFD system which is currently available at NLR are demonstrated. Transonic flow around the AS28G wing/body configuration and hypersonic flow through a generic three-dimensional mixed-compression airbreathing inlet are simulated. An assessment of the level of automation of the current CFD-system is made. The problem-turnaround time lies within the order of a week for both applications