Effectiveness of using different types of nuclei and methods of replanting queen bees in bags

In Ukraine, among other industries, beekeeping occupies an important place. This fact is facilitated by the developed agricultural direction of the country and the high percentage of cultivation of entomophilic crops. However, despite the broad prospects for development and transition to an industrial basis, the beekeeping industry of Ukraine is represented, in the vast majority, by relatively small farms. One of the reasons for the low development of industrial honey production in Ukraine is the high costs of organizing an industrial apiary, which include the purchase of modern equipment for quick and efficient opening of frames, special equipment for pumping honey, the construction of separate premises for the honey pumping workshop, as well as the constant updating of equipment, such as new hives and frames, and the purchase of breeding queens. Another significant problem is the death of bee colonies due to a variety of diseases, including Varroa mite infestation. The use of pesticides to combat diseases and pests of agricultural plants and forest lands contributes to its negative share. An effective and cost-effective way to expand and increase the productivity of the apiary is the formation of bee packages using high-quality breeding queens. The use of early fertile queens makes it possible to form a strong full-fledged family and make full use of the honey collection this year. The effectiveness of the use of different types of nuclei and methods of replanting fertile queen bees in the formation of bee packages was checked. The study was carried out on bees of the Ukrainian steppe breed. The best results in terms of the percentage of fertile queens and the cost of queens per fertile were obtained using a full-size nucleus for 3 standard frames in a 16-frame bed. However, the same method required a larger number (10 times) of worker bees compared to the micronucleus. With traditional replanting using the Titov cage, the share of accepted queen bees is 90 %, and the maximum rate of reception of queen bees in packages is recorded when using the fourth method of transplantation. So, when transferring a queen with a frame of printed brood and young worker bees, the relative proportion of accepted queen bees was 98 %. Therefore, the traditional method can be considered optimal in terms of indicators: reception, manufacturability, costs

СЕСТРИНСЬКИЙ ДОГЛЯД ЗА ХВОРИМИ З АСТЕНІЄЮ ПРИ ПАТОЛОГІЇ СИСТЕМИ КРОВІ

The article adduces the main causes of development pathophysiology peculiarities, diagnosis, treatment of the weakness syndrome and nursing care for patients with asthenia at diseases of the blood system.У статті розглянуто основні причини розвитку, особливості патофізіології, діагностика, лікування синдрому слабості та сестринський догляд за хворими з астенією при захворюваннях крові

Аналіз захворюваності на ГРВІ та COVID-19 особового складу окремої військової частини за 2020-2022 рокирр.

Мета – аналіз інфекційної захворюваності актуальними крапельними інфекціями, заходів імунопрофілактики та післявакцинальних реакцій на імунопрепарати проти COVID-19 серед особового складу окремого військового колективу ЗС України. Матеріали та методи. Використані законодавчі та нормативно-правові акти України з питань протиепідемічного забезпечення при виникненні інфекційних хвороб, а також форми обліку та звітності: 1/МЕД, журнали обліку первинних та вторинних звернень особового складу по медичну допомогу, відомості медичних книжок військовослужбовців, що ведуться у частині. Методи дослідження: бібліографічний, епідеміологічний, аналітичний, статистичний, клінічного спостереження. Результати. У період  з 2020 по 2022 роки було зареєстровано 13113 звернень по медичну допомогу з приводу захворювань на ГРВІ та COVІD-19 серед офіцерів, військовослужбовців контрактної служби, військовослужбовців строкової служби та держслужбовців (працівників) окремої військової частини (ОВЧ). Всього було зафіксовано за даний період 1760 випадків захворювання на COVІD-19. По рокам: за 2020 рік виявлено 399 хворих; за 2021 рік виявлено 717 хворих, за 2022 рік (за 9 місяців) виявлено 644 хворих на СOVID-19. В стаціонарних умовах було проліковано у згаданий період 122 особи, в амбулаторних умовах було проліковано 1638 осіб. Крім СOVID-19 у період з 2020 – 2022 рр. серед  усього особового складу ОВЧ було зафіксовано 10353 випадки захворювання на ГРВІ. Найбільша кількість звернень з приводу захворюваності на ГРВІ та COVID-19 була зареєстрована у 2021 році. Співвідношення ГРВІ до COVID-19 cклала 8:1.  Внаслідок відсутності вакцини, щеплення проти COVID-19 медичною службою частини у 2020 році не проводилось. У 2021 році було охоплено імунізацією вакциною різних виробників – 85% особового складу ОВЧ . Щодо ускладнень – спостерігалися загальновизнані реакції у вигляді підвищення температури до 37,5 - 38,00С, головного болю малої інтенсивності, незначні зниження концентрації уваги, які мали місце протягом першої половини наступної доби після ін’єкції. У поодиноких випадках, серед працівників ЗС України спостерігалися ускладнення середньої тяжкості у вигляді астеновегетативного синдрому, запаморочення, тахікардії, втрата апетиту. Реакція у місці введення препарату проявлялася головним чином у вигляді: незначного болю, гіперемією шкіри, ущільненням тканин навколо ін’єкції, незначним набряком та свербіжем. Найбільша кількість скарг щодо післявакцинальних реакцій надходила від осіб, які отримали щеплення вакциною СoronoVac та  Astra Zeneca. Висновки. Загальна кількість звернень по медичну допомогу з приводу захворювань на ГРВІ та COVІD-19 у період з 2020-2022 роки ОВЧ склала 13113 осіб, серед яких на COVID-19  ̶  1760 хворих (це 13,42% від загальної захворюваності, що майже відповідає середньому  показнику захворюваності в  Україні, який становив 12,4%). Кількість звернень з приводу ГРВІ склала 11353 випадків. Захворюваність головним чином було спричинена: не дотриманням масочного режиму, не правильним носінням масок та респіраторів, не дотриманням дистанції у громадських місцях, не своєчасним зверненням до лікарів, не дотриманням правил особистої гігієни. У 2021 році відсоток охоплення щепленням особового складу ОВЧ проти COVID-19 становив 85%. Темпи охоплення імунізацією у 2022 році дещо знизилися у порівнянні із 2021 роком, що може бути пов’язано із дотриманням термінів ревакцинації наступною (бустерною) дозами вакцини, бажанням проводити ревакцинацію імунопрепаратами конкретних виробників (вакциною Pfizer або Astra Zeneca)

Vaccination and Mortality of Patients with a Novel Coronavirus Infection (COVID-19): A Global Approach

Aim. The aim is to study the correlation between the vaccination rate (VR) and mortality rate of patients with COVID-19 (MpCOV).Methods. The countries with gross domestic product per capita corrected for purchasing power parity (GDP PPP) over $-10,000 were selected for an ecologic study. The city-states and countries with a population of 1,000,000 were excluded. The number of patients who died from COVID-19 within a week was divided by the number of patients diagnosed with COVID-19 within a week 20 days earlier to calculate MpCOV.Results. We included 85 countries. VR (r = –0.604; p < 0.001) and GDPpcPPP level (p = 0.202), is an independent determinant of MpCOV. There was no significant difference in MpCOV between groups of countries with VR < 20 % and 20–39 % (1.96 [1.21; 4.67] vs. 1.96 [1.01; 3.36] %; p = 0.464). MpCOV was higher in countries where VR were lower when groups of countries with VR of 20–39 %, 40–59 %, 60–79 %, and ≥80 % were compared (1.96 [1.01; 3.36] vs. 1.11 [0.76; 1.64] vs. 0.50 [0.39; 1.00] vs. 0.16 [0.10; 0.21]; p = 0.003; p = 0.020, and p = 0.008).Conclusions. An increase in VR correlates with a decrease in MpCOV

Biodiversity surveys of grassland and coastal habitats in 2021 as a documentation of pre-war status in southern Ukraine

Background This paper presents two sampling-event datasets with occurrences of vascular plants, bryophytes and lichens collected in May-June 2021 in southern Ukraine. We aimed to collect high-quality biodiversity data in an understudied region and contribute it to international databases and networks. The study was carried out during the 15th Eurasian Dry Grassland Group (EDGG) Field Workshop in southern Ukraine and the Dark Diversity Network (DarkDivNet) sampling in the Kamianska Sich National Nature Park. By chance, these datasets were collected shortly before the major escalation of the Russian invasion in Ukraine. Surveyed areas in Kherson and Mykolaiv Regions, including established monitoring plots, were severely affected by military actions in 2022. Therefore, collected data are of significant value in the context of biodiversity documentation. The knowledge about the biodiversity of this area will help to assess the environmental impact of the war and plan restoration of the damaged or destroyed habitats. The first preliminary analysis of collected data demonstrates the biodiversity richness and conservation value of studied grassland habitats. New information We provide sampling-event datasets with 7467 occurrences, which represent 708 taxa (vascular plants, bryophytes and lichens) collected in 275 vegetation relevés. Amongst them, vascular plants are represented by 6665 occurrences (610 taxa), lichens - 420 (46) and bryophytes - 381 (51). Several new species were reported for the first time at the national or regional level. In particular, one vascular plant species (Torilis pseudonodosa) and two lichen species (Cladonia conista, Endocarpon loscosii) were new to Ukraine. One vascular plant (Stipa tirsa), two species of bryophytes (Rhynchostegium megapolitanum, Ptychostomum torquescens) and three species of lichens (Cladonia cervicornis, C. symphycarpa, Involucropyrenium breussi) were recorded for the first time for the Kherson Region. Additionally, these datasets contain occurrences of taxa with narrow distribution, specialists of rare habitat types and, therefore, represented by a low number of occurrences in relevant biodiversity databases and particularly in GBIF. This publication highlights the diversity of natural vegetation and its flora in southern Ukraine and raises conservation concerns

Experience in genetic testing of hypertrophic cardiomyopathy using nanopore DNA sequencing

Aim. To investigate the application of the Oxford Nanopore Technologies’ third generation sequencing for the genetic testing of hypertrophic cardiomyopathy.Material and methods. The study involved 12 patients with hypertrophic cardiomyopathy aged 18 to 67 years (women, 9; men, 3). Using the PCR barcoding amplicons (SQK-LSK109) protocol, DNA libraries were created which contained long-range PCR fragments of the MYH7, MYBPC3, TNNT2, TNNI3 and TPM1 genes. The sequencing was performed using the MinION system by Oxford Nanopore Technologies (UK). Bioinformatic algorithms for data analysis included Guppy v.5.0.7, Nanopolish and Clairvoyante. The identified genetic variants were confirmed by Sanger sequencing.Results. Data on the complete sequence of the five major sarcomeric genes for hypertrophic cardiomyopathy were obtained. We found eight potentially disease-causing sequence variants in MYH7, MYBPC3 and TNNT2 genes by monomolecular sequencing. However, only three mutations p.Arg243Cys, p.Tyr609Asn, p.Arg870His in the MYH7 gene, and one mutation p.Lys985Asn in the MYBPC3 were confirmed by Sanger sequencing. Cascade screening of pathogenic variant p.Arg870His in the MYH7 gene was performed. We found one asymptomatic carrier.Conclusion. It appears that monomolecular sequencing technology is a feasible approach to identify mutations in patients with hypertrophic cardiomyopathy. Although improvement in accuracy of DNA sequencing, as well as optimization and simplification of bioinformatic algorithms for identification of the genetic variants are needed

Plant species richness records in Ukrainian steppes

We compiled a list of the known maxima of vascular plant species richness in the steppe zone of Ukraine at different sampling scales (0.0001–100 m2) and compared them with the world records. Detailed information on each of the identified species-rich grassland plots is provided, including site descriptions, plot characteristics, coordinates, and species lists. Most of the small-scale records (up to 0.1 m2) were found in mesic grasslands in southern Ukraine (Kherson region) with a high abundance of annual grasses and forbs: 9 and 11 vascular plant species in 0.0001 m2 and 0.001 m2, respectively, in the steppe depression Chorna Dolyna, and 26 species in 0.1 m2 in a mesic psammophytic grassland in the Dzharylhach National Nature Park. Most of the records at larger scales were made in the northern part of the steppe zone, in forb-rich and forb-grass steppes: 15, 39, 73, and 107 species in 0.01, 1, 10, and 100 m2, respectively. All richness records were characterised by neutral to slightly alkaline sub-strates (soil pH 7.0–7.7) and low-intensity management. These characteristics are consistent with the known patterns from world records of species richness. Our inventory provides baseline knowledge for studies on the high species richness in the steppe zone and can stimulate the further investigation of identified species-rich grasslands, as well as the search for new ones. Since we did not specifically search for species-rich sites, and our dataset includes only a relatively small number of plots compared to similar studies in other regions, we expect that more species-rich vegetation will be found with further research. This study highlights the importance of steppe grasslands as global biodiversity hotspots and draws attention to their protection in the context of land-use changes and the consequences of the war in Ukraine

Challenges in QCD matter physics - The Compressed Baryonic Matter experiment at FAIR

Substantial experimental and theoretical efforts worldwide are devoted to explore the phase diagram of strongly interacting matter. At LHC and top RHIC energies, QCD matter is studied at very high temperatures and nearly vanishing net-baryon densities. There is evidence that a Quark-Gluon-Plasma (QGP) was created at experiments at RHIC and LHC. The transition from the QGP back to the hadron gas is found to be a smooth cross over. For larger net-baryon densities and lower temperatures, it is expected that the QCD phase diagram exhibits a rich structure, such as a first-order phase transition between hadronic and partonic matter which terminates in a critical point, or exotic phases like quarkyonic matter. The discovery of these landmarks would be a breakthrough in our understanding of the strong interaction and is therefore in the focus of various high-energy heavy-ion research programs. The Compressed Baryonic Matter (CBM) experiment at FAIR will play a unique role in the exploration of the QCD phase diagram in the region of high net-baryon densities, because it is designed to run at unprecedented interaction rates. High-rate operation is the key prerequisite for high-precision measurements of multi-differential observables and of rare diagnostic probes which are sensitive to the dense phase of the nuclear fireball. The goal of the CBM experiment at SIS100 (sqrt(s_NN) = 2.7 - 4.9 GeV) is to discover fundamental properties of QCD matter: the phase structure at large baryon-chemical potentials (mu_B > 500 MeV), effects of chiral symmetry, and the equation-of-state at high density as it is expected to occur in the core of neutron stars. In this article, we review the motivation for and the physics programme of CBM, including activities before the start of data taking in 2022, in the context of the worldwide efforts to explore high-density QCD matter.Comment: 15 pages, 11 figures. Published in European Physical Journal

Ultrasound-facilitated, catheter-directed thrombolysis vs anticoagulation alone for acute intermediate-high-risk pulmonary embolism: Rationale and design of the HI-PEITHO study

BACKGROUND: Due to the bleeding risk of full-dose systemic thrombolysis and the lack of major trials focusing on the clinical benefits of catheter-directed treatment, heparin antiocoagulation remains the standard of care for patients with intermediate-high-risk pulmonary embolism (PE). METHODS AND RESULTS: The Higher-Risk Pulmonary Embolism Thrombolysis (HI-PEITHO) study (ClinicalTrials.gov Identifier: NCT04790370) is a multinational multicenter randomized controlled parallel-group comparison trial. Patients with: (1) confirmed acute PE; (2) evidence of right ventricular (RV) dysfunction on imaging; (3) a positive cardiac troponin test; and (4) clinical criteria indicating an elevated risk of early death or imminent hemodynamic collapse, will be randomized 1:1 to treatment with a standardized protocol of ultrasound-facilitated catheter-directed thrombolysis plus anticoagulation, vs anticoagulation alone. The primary outcome is a composite of PE-related mortality, cardiorespiratory decompensation or collapse, or non-fatal symptomatic and objectively confirmed PE recurrence, within 7 days of randomization. Further assessments cover, apart from bleeding complications, a broad spectrum of functional and patient-reported outcomes including quality of life indicators, functional status and the utilization of health care resources over a 12-month follow-up period. The trial plans to include 406 patients, but the adaptive design permits a sample size increase depending on the results of the predefined interim analysis. As of May 11, 2022, 27 subjects have been enrolled. The trial is funded by Boston Scientific Corporation and through collaborative research agreements with University of Mainz and The PERT Consortium. CONCLUSIONS: Regardless of the outcome, HI-PEITHO will establish the first-line treatment in intermediate-high risk PE patients with imminent hemodynamic collapse. The trial is expected to inform international guidelines and set the standard for evaluation of catheter-directed reperfusion options in the future