Efficient hepatitis C virus particle formation requires diacylglycerol acyltransferase-1.

Hepatitis C virus (HCV) infection is closely tied to the lipid metabolism of liver cells. Here we identify the triglyceride-synthesizing enzyme diacylglycerol acyltransferase-1 (DGAT1) as a key host factor for HCV infection. DGAT1 interacts with the viral nucleocapsid core and is required for the trafficking of core to lipid droplets. Inhibition of DGAT1 activity or RNAi-mediated knockdown of DGAT1 severely impairs infectious virion production, implicating DGAT1 as a new target for antiviral therapy

Static quantum corrections to the Schwarzschild spacetime

We study static quantum corrections of the Schwarzschild metric in the Boulware vacuum state. Due to the absence of a complete analytic expression for the full semiclassical Einstein equations we approach the problem by considering the s-wave approximation and solve numerically the associated backreaction equations. The solution, including quantum effects due to pure vacuum polarization, is similar to the classical Schwarzschild solution up to the vicinity of the classical horizon. However, the radial function has a minimum at a time-like surface close to the location of the classical event horizon. There the g_{00} component of the metric reaches a very small but non-zero value. The analysis unravels how a curvature singularity emerges beyond this bouncing point. We briefly discuss the physical consequences of these results by extrapolating them to a dynamical collapsing scenario.Comment: 10 pages; Talk given at QG05, Cala Gonone (Italy), September 200

Mapping the CMB Sky: The BOOMERANG experiment

We describe the BOOMERanG experiment, a stratospheric balloon telescope intended to measure the Cosmic Microwave Background anisotropy at angular scales between a few degrees and ten arcminutes. The experiment has been optimized for a long duration (7 to 14 days) flight circumnavigating Antarctica at the end of 1998. A test flight was performed on Aug.30, 1997 in Texas. The level of performance achieved in the test flight was satisfactory and compatible with the requirements for the long duration flight.Comment: 11 pages, 6 figure

Insulin but not phorbol ester treatment increases phosphorylation of vinculin by protein kinase C in BC3H-1 myocytes

AbstractInsulin was found to increase protein kinase C activity in BC3H-1 myocytes as determined by in vitro phosphorylation of both a lysine-rich histone fraction (histone III-S) and vinculin. TPA treatment for 20 min or 18 h provoked an apparent loss of histone-directed but not vinculin-directed phosphorylation by cytosolic C-kinase. Thus, chronic TPA-induced ‘desensitization’ or ‘depletion’ of cellular protein kinase C is more apparent than real, and is not a valid means for evaluating the role of C-kinase in hormone action

Model-Independent Comparisons of Pulsar Timings to Scalar-Tensor Gravity

Observations of pulsar timing provide strong constraints on scalar-tensor theories of gravity, but these constraints are traditionally quoted as limits on the microscopic parameters (like the Brans-Dicke coupling, for example) that govern the strength of scalar-matter couplings at the particle level in particular models. Here we present fits to timing data for several pulsars directly in terms of the phenomenological couplings (masses, scalar charges, moment of inertia sensitivities and so on) of the stars involved, rather than to the more microscopic parameters of a specific model. For instance, for the double pulsar PSR J0737-3039A/B we find at the 68% confidence level that the masses are bounded by 1.28 < m_A/m_sun < 1.34 and 1.19 < m_B/m_sun < 1.25, while the scalar-charge to mass ratios satisfy |a_A| < 0.21, |a_B| < 0.21 and |a_B - a_A| < 0.002$. These constraints are independent of the details of the scalar tensor model involved, and of assumptions about the stellar equations of state. Our fits can be used to constrain a broad class of scalar tensor theories by computing the fit quantities as functions of the microscopic parameters in any particular model. For the Brans-Dicke and quasi-Brans-Dicke models, the constraints obtained in this manner are consistent with those quoted in the literature.Comment: 19 pages, 7 figure

The BOOMERANG North America Instrument: a balloon-borne bolometric radiometer optimized for measurements of cosmic background radiation anisotropies from 0.3 to 4 degrees

We describe the BOOMERANG North America (BNA) instrument, a balloon-borne bolometric radiometer designed to map the Cosmic Microwave Background (CMB) radiation with 0.3 deg resolution over a significant portion of the sky. This receiver employs new technologies in bolometers, readout electronics, millimeter-wave optics and filters, cryogenics, scan and attitude reconstruction. All these subsystems are described in detail in this paper. The system has been fully calibrated in flight using a variety of techniques which are described and compared. It has been able to obtain a measurement of the first peak in the CMB angular power spectrum in a single balloon flight, few hours long, and was a prototype of the BOOMERANG Long Duration Balloon (BLDB) experiment.Comment: 40 pages, 22 figures, submitted to Ap

First Estimations of Cosmological Parameters From BOOMERANG

The anisotropy of the cosmic microwave background radiation contains information about the contents and history of the universe. We report new limits on cosmological parameters derived from the angular power spectrum measured in the first Antarctic flight of the BOOMERANG experiment. Within the framework of inflation-motivated adiabatic cold dark matter models, and using only weakly restrictive prior probabilites on the age of the universe and the Hubble expansion parameter $h$, we find that the curvature is consistent with flat and that the primordial fluctuation spectrum is consistent with scale invariant, in agreement with the basic inflation paradigm. We find that the data prefer a baryon density $\Omega_b h^2$ above, though similar to, the estimates from light element abundances and big bang nucleosynthesis. When combined with large scale structure observations, the BOOMERANG data provide clear detections of both dark matter and dark energy contributions to the total energy density $\Omega_{\rm {tot}}$, independent of data from high redshift supernovae.Comment: As submitted to PRD, revised longer version with an additional figur