Shaping and Structuring Supramolecular Gels

Supramolecular gels assemble via non-covalent interactions between low-molecular-weight gelators (LMWGs). The gels form a solid-like nanoscale network spanning a liquid-like continuous phase, translating molecular-scale information into materials performance. However, gels based on LMWGs are often difficult to manipulate, easily destroyed and have poor rheological performance. The recurring image of newly-discovered supramolecular gels is that of an inverted vial showing that the gel can support its own weight against gravity. Such images reflect the limitation that these gels simply fill the vessel in which they are made, with limited ability to be shaped. This property prevents supramolecular gels from having the same impact as polymer gels, despite greater synthetic tunability, reversibility and bio/environmental compatibility. In this Review, we evaluate strategies for imposing different shapes onto supramolecular gels and for patterning structures within them. We review fabrication methods including moulding, self-healing, 3D printing, photopatterning, diffusion and surface-mediated patterning. We discuss gelator chemistries amenable to each method, highlighting how a multi-component approach can aid shaping and structuring. Supramolecular gels with defined shapes, or patterned structures with precisely-controlled compositions, have the potential to intervene in applications such as tissue engineering and nanoscale electronics, as well as opening-up new technologies

Effect of Powder Particle Size on Solvent Free Membrane for 3D Hybrid Scaffold Structure

Solvent free membrane fabrication is a powder based method that can generate porous structure without used of organic solvent that can harmful to cell both in vitro and in vivo. In this paper, the effect of powder size on membrane morphology and surface wettability were investigated. Two different Poly (ε-caprolactone), (PCL) particle sizes of powder which are 100 µm and 500 µm were used. Powder was spread on top of water and by heating with laboratory oven in was melted down. After 10-15 minutes, it was fully melted and membrane can be obtained after cooled down. The powder was really affect morphology of membrane both macroscopic level and microscopic level. For macroscopic level, 500 µm membrane for different surface texture between water contact side and air contact side which air contact side gave smoother surface. On the other hand, for microscopic level, 100 µm showed more porous structure. Lastly, by further investigate the surface properties using contact angle, it confirmed that there is no significant between two different sides of 100 mm membrane but for 500 mm membrane it showed different manner because the contact angle results that was around 10 degree different between two sides.Published versio

Modifying the Properties of Thermogelling Poloxamer 407 Solutions through Covalent Modification and the Use of Polymer Additives

Thermoresponsive polymers that undergo sol–gel transition in a physiological temperature range have applications in biomedical science. Poloxamer 407 (P407) is commonly used as thermogelling material and has been approved by the Food and Drug Administration (FDA) in licenced medicines. However, it has significant drawbacks which limit its performance, particularly in drug delivery systems. In order to improve these properties, the chemical structure of P407 has been modified to produce stronger gels by either conjugating P407 with other polymers or introducing inter-micelle linkers to the terminal hydroxyl groups of P407. However, chemical modifications have several undesirable side-effects. The change in the chemical structure makes the polymer a novel excipient, and additional safety risks are possible, requiring expensive and time-consuming toxicity testing prior to regulatory approval. An alternative approach to covalent modification is modifying the P407 formulations with additives including hydrophilic polymers and nanoparticles, in an attempt to improve the properties of these materials. This review investigates the approaches used to modify the properties of P407 thermogelling materials, including the use of polymer additives and covalent modification. Several recommendations are made, based on efficacy and consideration of regulatory risk to guide the development of these materials toward use in real clinical applications.Peer reviewe

Multi-Scale Analysis of the Composition, Structure, and Function of Decellularized Extracellular Matrix for Human Skin and Wound Healing Models

The extracellular matrix (ECM) is a complex mixture of structural proteins, proteoglycans, and signaling molecules that are essential for tissue integrity and homeostasis. While a number of recent studies have explored the use of decellularized ECM (dECM) as a biomaterial for tissue engineering, the complete composition, structure, and mechanics of these materials remain incompletely understood. In this study, we performed an in-depth characterization of skin-derived dECM biomaterials for human skin equivalent (HSE) models. The dECM materials were purified from porcine skin, and through mass spectrometry profiling, we quantified the presence of major ECM molecules, including types I, III, and VI collagen, fibrillin, and lumican. Rheological analysis demonstrated the sol-gel and shear-thinning properties of dECM materials, indicating their physical suitability as a tissue scaffold, while electron microscopy revealed a complex, hierarchical structure of nanofibers in dECM hydrogels. The dECM materials were compatible with advanced biofabrication techniques, including 3D printing within a gelatin microparticle support bath, printing with a sacrificial material, or blending with other ECM molecules to achieve more complex compositions and structures. As a proof of concept, we also demonstrate how dECM materials can be fabricated into a 3D skin wound healing model using 3D printing. Skin-derived dECM therefore represents a complex and versatile biomaterial with advantageous properties for the fabrication of next-generation HSEs.</p