46 research outputs found
Biotransformation patterns of 2,4,6-trinitrotoluene by aerobic bacteria
2,4,6-Trinitrotoluene (TNT), a toxic nitroaromatic explosive, accumulates in the environment, making necessary the remediation of contaminated areas and unused materials. Although bioremediation has been utilized to detoxify TNT, the metabolic process involved in the metabolism of TNT have proven to be complex. The three aerobic bacterial strains reported here (Pseudomonas aeruginosa, Bacillus sp., and Staphylococcus sp.) differ in their ability to biotransform TNT and in their growth characteristics in the presence of TNT. In addition, enzymatic activities have been identified that differ in the reduction of nitro groups, cofactor preferences, and the ability to eliminate-NO2 from the ring. The Bacillus sp. has the most diverse bioremediation potential owing to its growth in the presence of TNT, high level of reductive ability, and capability of removing-NO2 from the nitroaromatic ring
Amino-nitrile cleavage in the electrochemical reduction of hydeazones of aromatic aldehydes
1. Factors which determine the possibility of amino-nitrile cleavage of hydrazones on electrochemical reduction (ECR) include the basicity of the anionic product formed in the course of the ECR and the mobility of the aldehyde hydrogen which depends on the character of the electron polarization of the hydrazone fragment and the polarity of the N-N bond. 2. The primary action in amino-nitrile cleavage under conditions of ECR is the deprotonation of the azomethine fragment in the unreduced molecule by electrochemically generated strong base (anion or dianion). Β© 1988 Plenum Publishing Corporation
The transition problems from traditional physical trainings to the trainings based on interests
Π ΡΡΠ°ΡΡΠ΅ ΡΠ°ΡΡΠΌΠΎΡΡΠ΅Π½Ρ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΡΠ΅ ΠΈ ΡΡΡΠ΅ΡΡΠ²ΡΡΡΠΈΠ΅ ΠΏΡΠΎΠ±Π»Π΅ΠΌΡ ΠΏΠ΅ΡΠ΅Ρ
ΠΎΠ΄Π° ΠΎΡ ΡΡΠ°Π΄ΠΈΡΠΈΠΎΠ½Π½ΠΎΠΉ ΡΠΎΡΠΌΡ Π·Π°Π½ΡΡΠΈΠΉ ΡΠΈΠ·ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΊΡΠ»ΡΡΡΡΠΎΠΉ ΠΊ Π·Π°Π½ΡΡΠΈΡΠΌ ΡΠΈΠ·ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΊΡΠ»ΡΡΡΡΠΎΠΉ ΠΏΠΎ ΠΈΠ½ΡΠ΅ΡΠ΅ΡΠ°ΠΌThis article describes possible and existing transition problems from traditional physical trainings to the trainings based on interest
Increasing productivity of arylsulfatase B-producing cell line by coexpression of formylglycine-generating enzyme
Mucopolysaccharidosis type VI (MaroteauxβLamy syndrome) is an orphan genetic disease caused by deficiency of the lysosomal enzyme arylsulfatase B (ASB). The need to develop a highly productive cell line for the production of recombinant ASB, is behind the concept and relevance of this study. The most promising approach seems to be the development of CHO producer cell lines coexpressing the target ASB enzyme and an auxiliary formylglycine-generating enzyme (FGE). At the same time, it is important from a practical perspective to have the possibility of cultivating producer cell lines as suspensions free of serum or other components of animal origin. The aim of the study was to develop highly productive cell lines for the production of recombinant ASB by coexpression of the auxiliary FGE. Materials and methods: a suspension CHO cell line was used in the study. CHO cells were transfected by electroporation using the MaxCyte STX system. Monoclonal cell lines were obtained with the help of the Cell Metric system. Enzyme-linked immunosorbent assay was used for determination of ASB concentration in the culture fluid. Culture fluid samples were analysed using polyacrylamide gel electrophoresis and Western blotting. The mRNA level was measured by real-time polymerase chain reaction. Results: producer cell lines coexpressing the target ASB enzyme and auxiliary FGE were obtained. An increase in the yield of the active target ASB enzyme from 2 to 100 mg/L was achieved by selecting the optimal ratio of plasmids during transfection. The highest yield of the target ASB enzyme was achieved at the 90:10 ratio (%) of plasmids encoding the ASB and FGE genes, respectively. Conclusions: the authors developed highly productive cell lines for the production of recombinant ASB, which coexpress the target and auxiliary enzymes. The coexpression of ASB and FGE improves the growth and production characteristics of the cell line, probably due to the modification of the ASB active site. The obtained results will help resolve the problem of low enzyme yield, which is typical of this class of medicines
Π£Π²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΉ Π»ΠΈΠ½ΠΈΠΈ-ΠΏΡΠΎΠ΄ΡΡΠ΅Π½ΡΠ° Π°ΡΠΈΠ»ΡΡΠ»ΡΡΠ°ΡΠ°Π·Ρ B Π·Π° ΡΡΠ΅Ρ ΠΊΠΎΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ ΡΠΎΡΠΌΠΈΠ»Π³Π»ΠΈΡΠΈΠ½-Π³Π΅Π½Π΅ΡΠΈΡΡΡΡΠ΅Π³ΠΎ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°
Mucopolysaccharidosis type VI (MaroteauxβLamy syndrome) is an orphan genetic disease caused by deficiency of the lysosomal enzyme arylsulfatase B (ASB). The need to develop a highly productive cell line for the production of recombinant ASB, is behind the concept and relevance of this study. The most promising approach seems to be the development of CHO producer cell lines coexpressing the target ASB enzyme and an auxiliary formylglycine-generating enzyme (FGE). At the same time, it is important from a practical perspective to have the possibility of cultivating producer cell lines as suspensions free of serum or other components of animal origin. The aim of the study was to develop highly productive cell lines for the production of recombinant ASB by coexpression of the auxiliary FGE. Materials and methods: a suspension CHO cell line was used in the study. CHO cells were transfected by electroporation using the MaxCyte STX system. Monoclonal cell lines were obtained with the help of the Cell Metric system. Enzyme-linked immunosorbent assay was used for determination of ASB concentration in the culture fluid. Culture fluid samples were analysed using polyacrylamide gel electrophoresis and Western blotting. The mRNA level was measured by real-time polymerase chain reaction. Results: producer cell lines coexpressing the target ASB enzyme and auxiliary FGE were obtained. An increase in the yield of the active target ASB enzyme from 2 to 100 mg/L was achieved by selecting the optimal ratio of plasmids during transfection. The highest yield of the target ASB enzyme was achieved at the 90:10 ratio (%) of plasmids encoding the ASB and FGE genes, respectively. Conclusions: the authors developed highly productive cell lines for the production of recombinant ASB, which coexpress the target and auxiliary enzymes. The coexpression of ASB and FGE improves the growth and production characteristics of the cell line, probably due to the modification of the ASB active site. The obtained results will help resolve the problem of low enzyme yield, which is typical of this class of medicines.ΠΡΠΊΠΎΠΏΠΎΠ»ΠΈΡΠ°Ρ
Π°ΡΠΈΠ΄ΠΎΠ· VI ΡΠΈΠΏΠ° (ΡΠΈΠ½Π΄ΡΠΎΠΌ ΠΠ°ΡΠΎΡΠΎβΠΠ°ΠΌΠΈ) β ΠΎΡΡΠ°Π½Π½ΠΎΠ΅ Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠ΅ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠ΅, ΠΊΠΎΡΠΎΡΠΎΠ΅ ΡΠ²ΡΠ·Π°Π½ΠΎ Ρ Π΄Π΅ΡΠΈΡΠΈΡΠΎΠΌ Π»ΠΈΠ·ΠΎΡΠΎΠΌΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ° Π°ΡΠΈΠ»ΡΡΠ»ΡΡΠ°ΡΠ°Π·Ρ Π. ΠΠΊΡΡΠ°Π»ΡΠ½ΠΎΡΡΡ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΡΠ²ΡΠ·Π°Π½Π° Ρ Π½Π΅ΠΎΠ±Ρ
ΠΎΠ΄ΠΈΠΌΠΎΡΡΡΡ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠΊΠΈ Π²ΡΡΠΎΠΊΠΎΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠ²Π½ΠΎΠΉ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΉ Π»ΠΈΠ½ΠΈΠΈ-ΠΏΡΠΎΠ΄ΡΡΠ΅Π½ΡΠ° ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½ΡΠ½ΠΎΠ³ΠΎ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ° Π°ΡΠΈΠ»ΡΡΠ»ΡΡΠ°ΡΠ°Π·Ρ Π. ΠΠ°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΠΏΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²Π½ΡΠΌ ΠΏΠΎΠ΄Ρ
ΠΎΠ΄ΠΎΠΌ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΠ΅ΡΡΡ ΡΠΎΠ·Π΄Π°Π½ΠΈΠ΅ ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΡ
Π»ΠΈΠ½ΠΈΠΉ-ΠΏΡΠΎΠ΄ΡΡΠ΅Π½ΡΠΎΠ², ΠΊΠΎΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡΡΡΡΠΈΡ
ΡΠ΅Π»Π΅Π²ΠΎΠΉ ΡΠ΅ΡΠΌΠ΅Π½Ρ Π°ΡΠΈΠ»ΡΡΠ»ΡΡΠ°ΡΠ°Π·Ρ Π ΠΈ Π²ΡΠΏΠΎΠΌΠΎΠ³Π°ΡΠ΅Π»ΡΠ½ΡΠΉ ΡΠΎΡΠΌΠΈΠ»Π³Π»ΠΈΡΠΈΠ½-Π³Π΅Π½Π΅ΡΠΈΡΡΡΡΠΈΠΉ ΡΠ΅ΡΠΌΠ΅Π½Ρ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΉ Π»ΠΈΠ½ΠΈΠΈ Π‘ΠΠ. ΠΡΠΈ ΡΡΠΎΠΌ Π±ΠΎΠ»ΡΡΠΎΠ΅ ΠΏΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΎΠ΅ Π·Π½Π°ΡΠ΅Π½ΠΈΠ΅ ΠΈΠΌΠ΅Π΅Ρ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΡ ΠΊΡΠ»ΡΡΠΈΠ²ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΡ
Π»ΠΈΠ½ΠΈΠΉ-ΠΏΡΠΎΠ΄ΡΡΠ΅Π½ΡΠΎΠ² Π² Π²ΠΈΠ΄Π΅ ΡΡΡΠΏΠ΅Π½Π·ΠΈΠΈ, Π±Π΅Π· ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΡ ΡΡΠ²ΠΎΡΠΎΡΠΊΠΈ ΠΈΠ»ΠΈ Π΄ΡΡΠ³ΠΈΡ
ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠΎΠ² ΠΆΠΈΠ²ΠΎΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΎΠΈΡΡ
ΠΎΠΆΠ΄Π΅Π½ΠΈΡ. Π¦Π΅Π»Ρ ΡΠ°Π±ΠΎΡΡ: ΡΠ°Π·ΡΠ°Π±ΠΎΡΠΊΠ° Π²ΡΡΠΎΠΊΠΎΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠ²Π½ΡΡ
ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΡ
Π»ΠΈΠ½ΠΈΠΉ-ΠΏΡΠΎΠ΄ΡΡΠ΅Π½ΡΠΎΠ² ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½ΡΠ½ΠΎΠ³ΠΎ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ° Π°ΡΠΈΠ»ΡΡΠ»ΡΡΠ°ΡΠ°Π·Ρ Π Π·Π° ΡΡΠ΅Ρ ΠΊΠΎΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π²ΡΠΏΠΎΠΌΠΎΠ³Π°ΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠΎΡΠΌΠΈΠ»Π³Π»ΠΈΡΠΈΠ½-Π³Π΅Π½Π΅ΡΠΈΡΡΡΡΠ΅Π³ΠΎ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°. ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ: ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π»ΠΈ ΡΡΡΠΏΠ΅Π½Π·ΠΈΠΎΠ½Π½ΡΡ ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΡ Π»ΠΈΠ½ΠΈΡ Π‘ΠΠ. Π’ΡΠ°Π½ΡΡΠ΅ΠΊΡΠΈΡ ΠΊΠ»Π΅ΡΠΎΠΊ Π‘ΠΠ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΠ»Π΅ΠΊΡΡΠΎΠΏΠΎΡΠ°ΡΠΈΠΈ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΡΠΈΡΡΠ΅ΠΌΡ MaxCyte STX. ΠΠΎΠ½ΠΎΠΊΠ»ΠΎΠ½Π°Π»ΡΠ½ΡΠ΅ ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΠ΅ Π»ΠΈΠ½ΠΈΠΈ ΠΏΠΎΠ»ΡΡΠ°Π»ΠΈ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΡΠΈΡΡΠ΅ΠΌΡ Cell Metric. ΠΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΡ Π°ΡΠΈΠ»ΡΡΠ»ΡΡΠ°ΡΠ°Π·Ρ Π Π² ΠΊΡΠ»ΡΡΡΡΠ°Π»ΡΠ½ΠΎΠΉ ΠΆΠΈΠ΄ΠΊΠΎΡΡΠΈ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΈΠΌΠΌΡΠ½ΠΎΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π°. ΠΠ±ΡΠ°Π·ΡΡ ΠΊΡΠ»ΡΡΡΡΠ°Π»ΡΠ½ΠΎΠΉ ΠΆΠΈΠ΄ΠΊΠΎΡΡΠΈ Π°Π½Π°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π»ΠΈ Ρ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ ΡΠ»Π΅ΠΊΡΡΠΎΡΠΎΡΠ΅Π·Π° Π² ΠΏΠΎΠ»ΠΈΠ°ΠΊΡΠΈΠ»Π°ΠΌΠΈΠ΄Π½ΠΎΠΌ Π³Π΅Π»Π΅ ΠΈ Π²Π΅ΡΡΠ΅ΡΠ½-Π±Π»ΠΎΡΠ°. Π£ΡΠΎΠ²Π΅Π½Ρ ΠΌΠ ΠΠ ΠΈΠ·ΠΌΠ΅ΡΡΠ»ΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠ°Π·Π½ΠΎΠΉ ΡΠ΅ΠΏΠ½ΠΎΠΉ ΡΠ΅Π°ΠΊΡΠΈΠΈ Π² ΡΠ΅ΠΆΠΈΠΌΠ΅ ΡΠ΅Π°Π»ΡΠ½ΠΎΠ³ΠΎ Π²ΡΠ΅ΠΌΠ΅Π½ΠΈ. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ: ΠΏΠΎΠ»ΡΡΠ΅Π½Ρ ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΠ΅ Π»ΠΈΠ½ΠΈΠΈ-ΠΏΡΠΎΠ΄ΡΡΠ΅Π½ΡΡ, ΠΊΠΎΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡΡΡΡΠΈΠ΅ ΡΠ΅Π»Π΅Π²ΠΎΠΉ ΡΠ΅ΡΠΌΠ΅Π½Ρ Π°ΡΠΈΠ»ΡΡΠ»ΡΡΠ°ΡΠ°Π·Ρ Π ΠΈ Π²ΡΠΏΠΎΠΌΠΎΠ³Π°ΡΠ΅Π»ΡΠ½ΡΠΉ ΡΠΎΡΠΌΠΈΠ»Π³Π»ΠΈΡΠΈΠ½-Π³Π΅Π½Π΅ΡΠΈΡΡΡΡΠΈΠΉ ΡΠ΅ΡΠΌΠ΅Π½Ρ. ΠΠΎΡΡΠΈΠ³Π½ΡΡΠΎ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ Π²ΡΡ
ΠΎΠ΄Π° Π°ΠΊΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΡΠ΅Π»Π΅Π²ΠΎΠ³ΠΎ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ° Π°ΡΠΈΠ»ΡΡΠ»ΡΡΠ°ΡΠ°Π·Ρ Π Ρ 2 Π΄ΠΎ 100 ΠΌΠ³/Π» Π·Π° ΡΡΠ΅Ρ ΠΏΠΎΠ΄Π±ΠΎΡΠ° ΠΎΠΏΡΠΈΠΌΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠΎΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΡ ΠΏΠ»Π°Π·ΠΌΠΈΠ΄ Π²ΠΎ Π²ΡΠ΅ΠΌΡ ΡΡΠ°Π½ΡΡΠ΅ΠΊΡΠΈΠΈ. ΠΠ°ΠΈΠ±ΠΎΠ»ΡΡΠΈΠΉ Π²ΡΡ
ΠΎΠ΄ ΡΠ΅Π»Π΅Π²ΠΎΠ³ΠΎ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ° Π°ΡΠΈΠ»ΡΡΠ»ΡΡΠ°ΡΠ°Π·Ρ Π Π½Π°Π±Π»ΡΠ΄Π°Π»ΡΡ ΠΏΡΠΈ ΡΠΎΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΠΈ ΠΏΠ»Π°Π·ΠΌΠΈΠ΄, ΠΊΠΎΠ΄ΠΈΡΡΡΡΠΈΡ
Π³Π΅Π½Ρ Π°ΡΠΈΠ»ΡΡΠ»ΡΡΠ°ΡΠ°Π·Ρ Π ΠΈ ΡΠΎΡΠΌΠΈΠ»Π³Π»ΠΈΡΠΈΠ½-Π³Π΅Π½Π΅ΡΠΈΡΡΡΡΠ΅Π³ΠΎ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°, ΡΠ°Π²Π½ΠΎΠΌ 90:10 (%). ΠΡΠ²ΠΎΠ΄Ρ: ΡΠ°Π·ΡΠ°Π±ΠΎΡΠ°Π½Ρ Π²ΡΡΠΎΠΊΠΎΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠ²Π½ΡΠ΅ ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΠ΅ Π»ΠΈΠ½ΠΈΠΈ-ΠΏΡΠΎΠ΄ΡΡΠ΅Π½ΡΡ ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½ΡΠ½ΠΎΠ³ΠΎ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ° Π°ΡΠΈΠ»ΡΡΠ»ΡΡΠ°ΡΠ°Π·Ρ Π, ΠΊΠΎΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡΡΡΡΠΈΠ΅ ΡΠ΅Π»Π΅Π²ΠΎΠΉ ΠΈ Π²ΡΠΏΠΎΠΌΠΎΠ³Π°ΡΠ΅Π»ΡΠ½ΡΠΉ ΡΠ΅ΡΠΌΠ΅Π½ΡΡ. ΠΠΎΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ Π°ΡΠΈΠ»ΡΡΠ»ΡΡΠ°ΡΠ°Π·Ρ Π ΠΈ ΡΠΎΡΠΌΠΈΠ»Π³Π»ΠΈΡΠΈΠ½-Π³Π΅Π½Π΅ΡΠΈΡΡΡΡΠ΅Π³ΠΎ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ° ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΡ ΠΊ ΡΠ»ΡΡΡΠ΅Π½ΠΈΡ ΡΠΎΡΡΠΎΠ²ΡΡ
ΠΈ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠΎΠ½Π½ΡΡ
Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΉ Π»ΠΈΠ½ΠΈΠΈ, ΡΡΠΎ, ΠΏΠΎ-Π²ΠΈΠ΄ΠΈΠΌΠΎΠΌΡ, ΠΎΠ±ΡΡΠ»ΠΎΠ²Π»Π΅Π½ΠΎ ΠΌΠΎΠ΄ΠΈΡΠΈΠΊΠ°ΡΠΈΠ΅ΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΡΠ΅Π½ΡΡΠ° ΡΠ΅Π»Π΅Π²ΠΎΠ³ΠΎ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ° Π°ΡΠΈΠ»ΡΡΠ»ΡΡΠ°ΡΠ°Π·Ρ Π. ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡ ΡΠ΅ΡΠΈΡΡ ΠΏΡΠΎΠ±Π»Π΅ΠΌΡ Π½ΠΈΠ·ΠΊΠΎΠ³ΠΎ Π²ΡΡ
ΠΎΠ΄Π° ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°, Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠ½ΡΡ Π΄Π»Ρ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ² ΠΏΠΎΠ΄ΠΎΠ±Π½ΠΎΠ³ΠΎ ΠΊΠ»Π°ΡΡΠ°
Martensite-to-austenite reversion and recrystallization in cryogenically-rolled type 321 metastable austenitic steel
The annealing behavior of cryogenically-rolled type 321 metastable austenitic steel was established. Cryogenic deformation gave rise to martensitic transformation which developed preferentially within deformation bands. Subsequent annealing in the range of 600 C to 700 C resulted in reversion of the strain-induced martensite to austenite. At 800 C, the reversion was followed by static recrystallization. At relatively-low temperatures, the reversion was characterized by a very strong variant selection, which led to the restoration of the crystallographic orientation of the coarse parent austenite grains. An increase in the annealing temperature relaxed the variant-selection tendency and provided subsequent recrystallization thus leading to significant grain refinement. Nevertheless, a significant portion of the original coarse grains was found to be untransformed and therefore the fine-grain structure was fairly heterogeneous
EBSD characterization of cryogenically rolled type 321 austenitic stainless steel
Electron backscatter diffraction was applied to investigate microstructure evolution during cryogenic rolling of type 321 metastable austenitic stainless steel. As expected, rolling promoted deformation-induced martensitic transformation which developed preferentially in deformation bands. Because a large fraction of the imposed strain was accommodated by deformation banding, grain refinement in the parent austenite phase was minimal. The martensitic transformation was found to follow a general orientation relationship, {111}Ξ³||{0001}Ξ΅||{110}Ξ±β² and γ110γΞ³||γ11-20γΞ΅||γ111γΞ±β², and was characterized by noticeable variant selection