8,425 research outputs found
Introduction and Expression of a Rabbit β-globin Gene in Mouse Fibroblasts
The cloned chromosomal rabbit ß-globin gene has been introduced into mouse fibroblasts by DNA-mediated gene transfer (transformation). In this report, we examine the expression of the rabbit gene in six independent transformants that contain from 1 to 20 copies of the cloned globin gene. Rabbit globin transcripts were detected in two of these transformants at steady-state concentrations of 5 and 2 copies per cell. The globin transcripts from one cell line are polyadenylylated and migrate as 9S RNA on methylmercury gels. These transcripts reflect correct processing of the two intervening sequences but lack 48 ± 5 nucleotides present at the 5' terminus of rabbit erythrocyte globin mRNA
Study of resonance light scattering for remote optical probing
Enhanced scattering and fluorescence processes in the visible and UV were investigated which will enable improved remote measurements of gas properties. The theoretical relationship between scattering and fluorescence from an isolated molecule in the approach to resonance is examined through analysis of the time dependence of re-emitted light following excitation of pulsed incident light. Quantitative estimates are developed for the relative and absolute intensities of fluorescence and resonance scattering. New results are obtained for depolarization of scattering excited by light at wavelengths within a dissociative continuum. The experimental work was performed in two separate facilities. One of these utilizes argon and krypton lasers, single moded by a tilted etalon, and a 3/4 meter double monochromator. This facility was used to determine properties of the re-emission from NO2, I2 and O3 excited by visible light. The second facility involves a narrow-line dye laser, and a 3/4 meter single monochromator. The dye laser produces pulsed light with 5 nsec pulse duration and 0.005 nm spectral width
A New Handle on de Sitter Compactifications
We construct a large new class of de Sitter (and anti de Sitter) vacua of
critical string theory from flux compactifications on products of Riemann
surfaces. In the construction, the leading effects stabilizing the moduli are
perturbative. We show that these effects self-consistently dominate over
standard estimates for further and quantum corrections, via
tuning available from large flux and brane quantum numbers.Comment: 26 pages, harvmac big. v2: Correction generalizing specific
ingredients required for tunable negative term; conclusions and structure of
potential unchange
The Scaling of the No Scale Potential and de Sitter Model Building
We propose a variant of the KKLT (A)dS flux vacuum construction which does
not require an antibrane to source the volume modulus. The strategy is to find
nonzero local minima of the no-scale potential in the complex structure and
dilaton directions in moduli space. The corresponding no-scale potential
expanded about this point sources the volume modulus in the same way as does
the antibrane of the KKLT construction. We exhibit explicit examples of such
nonzero local minima of the no-scale potential in a simple toroidal orientifold
model.Comment: 11 pages, harvmac big. v2: trivial typos fixe
4d Conformal Field Theories and Strings on Orbifolds
We propose correspondences between 4d quantum field theories with N=2,1,0
(super)conformal invariance and Type IIB string theory on various orbifolds. We
argue using the spacetime string theory, and check using the beta functions
(exactly for N=2,1 and so far at 1-loop for the gauge couplings in the N=0
case), that these theories have conformal fixed lines. The latter case
potentially gives well-defined non-supersymmetric vacua of string theory, with
a mechanism for making the curvature and cosmological constant small at
nontrivial string coupling. We suggest a correspondence between
nonsupersymmetric conformal fixed lines and nonsupersymmetric string vacua with
vanishing vacuum energy.Comment: 11 pages, harvmac big. Reference adde
On the Matrix Description of Calabi-Yau Compactifications
We point out that the matrix description of M-theory compactified on
Calabi-Yau threefolds is in many respects simpler than the matrix description
of a compactification. This is largely because of the differences between
D6 branes wrapped on Calabi-Yau threefolds and D6 branes wrapped on six-tori.
In particular, if we define the matrix theory following the prescription of Sen
and Seiberg, we find that the remaining degrees of freedom are decoupled from
gravity.Comment: 12 pages, harvmac big; comment on 4d N=1 theories change
Simple de Sitter Solutions
We present a framework for de Sitter model building in type IIA string
theory, illustrated with specific examples. We find metastable dS minima of the
potential for moduli obtained from a compactification on a product of two Nil
three-manifolds (which have negative scalar curvature) combined with
orientifolds, branes, fractional Chern-Simons forms, and fluxes. As a discrete
quantum number is taken large, the curvature, field strengths, inverse volume,
and four dimensional string coupling become parametrically small, and the de
Sitter Hubble scale can be tuned parametrically smaller than the scales of the
moduli, KK, and winding mode masses. A subtle point in the construction is that
although the curvature remains consistently weak, the circle fibers of the
nilmanifolds become very small in this limit (though this is avoided in
illustrative solutions at modest values of the parameters). In the simplest
version of the construction, the heaviest moduli masses are parametrically of
the same order as the lightest KK and winding masses. However, we provide a
method for separating these marginally overlapping scales, and more generally
the underlying supersymmetry of the model protects against large corrections to
the low-energy moduli potential.Comment: 37 pages, harvmac big, 4 figures. v3: small correction
Enhancement of the ferromagnetic order of graphite after sulphuric acid treatment
We have studied the changes in the ferromagnetic behavior of graphite powder
and graphite flakes after treatment with diluted sulphuric acid. We show that
this kind of acid treatment enhances substantially the ferromagnetic
magnetization of virgin graphite micrometer size powder as well as in graphite
flakes. The anisotropic magnetoresistance (AMR) amplitude at 300 K measured in
a micrometer size thin graphite flake after acid treatment reaches values
comparable to polycrystalline cobalt.Comment: 3.2 pages, 4 figure
Activated platelets form protected zones of adhesion on fibrinogen and fibronectin-coated surfaces.
Leukocytes form zones of close apposition when they adhere to ligand-coated surfaces. Because plasma proteins are excluded from these contact zones, we have termed them protected zones of adhesion. To determine whether platelets form similar protected zones of adhesion, gel-filtered platelets stimulated with thrombin or ADP were allowed to adhere to fibrinogen- or fibronectin-coated surfaces. The protein-coated surfaces with platelets attached were stained with either fluorochrome-conjugated goat anti-human fibrinogen or anti-human fibronectin antibodies, or with rhodamine-conjugated polyethylene glycol polymers. Fluorescence microscopy revealed that F(ab')2 anti-fibrinogen (100 kD) did not penetrate into the contact zones between stimulated platelets and the underlying fibrinogen-coated surface, while Fab antifibrinogen (50 kD) and 10 kD polyethylene glycol readily penetrated and stained the substrate beneath the platelets. Thrombin- or ADP-stimulated platelets also formed protected zones of adhesion on fibronectin-coated surfaces. F(ab')2 anti-fibronectin and 10 kD polyethylene glycol were excluded from these adhesion zones, indicating that they are much less permeable than those formed by platelets on fibrinogen-coated surfaces. The permeability properties of protected zones of adhesion formed by stimulated platelets on surfaces coated with both fibrinogen and fibronectin were similar to the zones of adhesion formed on fibronectin alone. mAb 7E3, directed against the alpha IIb beta 3 integrin blocked the formation of protected adhesion zones between thrombin-stimulated platelets and fibrinogen or fibronectin coated surfaces. mAb C13 is directed against the alpha 5 beta 1 integrin on platelets. Stimulated platelets treated with this mAb formed protected zones of adhesion on surfaces coated with fibronectin. These protected zones were impermeable to F(ab')2 antifibronectin but were permeable to 10 kD polyethylene glycol. These results show that activated platelets form protected zones of adhesion and that the size of molecules excluded from these zones depends upon the composition of the matrix proteins to which the platelets adhere. They also show that formation of protected zones of adhesion by platelets requires alpha IIb beta 3 integrins while the permeability properties of these zones of adhesion are regulated by both alpha IIb beta 3 and alpha 5 beta 1 integrins
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