Genetic Detection and a Method to Study the Ecology of Deadly Cubozoan Jellyfish

Cubozoan jellyfish pose a risk of envenomation to humans and a threat to many businesses, yet crucial gaps exist in determining threats to stakeholders and understanding their ecology. Environmental DNA (eDNA) provides a cost-effective method for detection that is less labour intensive and provides a higher probability of detection. The objective of this study was to develop, optimise and trial the use of eDNA to detect the Australian box jellyfish, Chironex fleckeri. This species was the focus of this study as it is known to have the strongest venom of any cubozoan; it is responsible for more than 200 recorded deaths in the Indo-Pacific region. Further, its ecology is poorly known. Herein, a specific and sensitive probe-based assay, multiplexed with an endogenous control assay, was developed, and successfully utilised to detect the deadly jellyfish species and differentiate them from closely related taxa. A rapid eDNA decay rate of greater than 99% within 27 h was found with no detectable influence from temperature. The robustness of the technique indicates that it will be of high utility for detection and to address knowledge gaps in the ecology of C. fleckeri; further, it has broad applicability to other types of zooplankton

Crack Front Waves and the dynamics of a rapidly moving crack

Crack front waves are localized waves that propagate along the leading edge of a crack. They are generated by the interaction of a crack with a localized material inhomogeneity. We show that front waves are nonlinear entities that transport energy, generate surface structure and lead to localized velocity fluctuations. Their existence locally imparts inertia, which is not incorporated in current theories of fracture, to initially "massless" cracks. This, coupled to crack instabilities, yields both inhomogeneity and scaling behavior within fracture surface structure.Comment: Embedded Latex file including 4 figure

Single shot measurement of a silicon single electron transistor

We have fabricated a custom cryogenic Complementary Metal-Oxide-Semiconductor (CMOS) integrated circuit that has a higher measurement bandwidth compared with conventional room temperature electronics. This allowed implementing single shot operations and observe the real-time evolution of the current of a phosphorous-doped silicon single electron transistor that was irradiated with a microwave pulse. Relaxation times up to 90 us are observed, suggesting the presence of well isolated electron excitations within the device. It is expected that these are associated with long decoherence time and the device may be suitable for quantum information processing

Cosmic Strings from Supersymmetric Flat Directions

Flat directions are a generic feature of the scalar potential in supersymmetric gauge field theories. They can arise, for example, from D-terms associated with an extra abelian gauge symmetry. Even when supersymmetry is broken softly, there often remain directions in the scalar field space along which the potential is almost flat. Upon breaking a gauge symmetry along one of these almost flat directions, cosmic strings may form. Relative to the standard cosmic string picture based on the abelian Higgs model, these flat-direction cosmic strings have the extreme Type-I properties of a thin gauge core surrounded by a much wider scalar field profile. We perform a comprehensive study of the microscopic, macroscopic, and observational characteristics of this class of strings. We find many differences from the standard string scenario, including stable higher winding mode strings, the dynamical formation of higher mode strings from lower ones, and a resultant multi-tension scaling string network in the early universe. These strings are only moderately constrained by current observations, and their gravitational wave signatures may be detectable at future gravity wave detectors. Furthermore, there is the interesting but speculative prospect that the decays of cosmic string loops in the early universe could be a source of ultra-high energy cosmic rays or non-thermal dark matter. We also compare the observational signatures of flat-direction cosmic strings with those of ordinary cosmic strings as well as (p,q) cosmic strings motivated by superstring theory.Comment: 58 pages, 16 figures, v2. accepted to PRD, added comments about baryogenesis and boosted decay products from cusp annihilatio

Enhanced Production of Neutron-Rich Rare Isotopes in Peripheral Collisions at Fermi Energies

A large enhancement in the production of neutron-rich projectile residues is observed in the reactions of a 25 MeV/nucleon 86Kr beam with the neutron rich 124Sn and 64Ni targets relative to the predictions of the EPAX parametrization of high-energy fragmentation, as well as relative to the reaction with the less neutron-rich 112Sn target. The data demonstrate the significant effect of the target neutron-to-proton ratio (N/Z) in peripheral collisions at Fermi energies. A hybrid model based on a deep-inelastic transfer code (DIT) followed by a statistical de-excitation code appears to account for part of the observed large cross sections. The DIT simulation indicates that the production of the neutron-rich nuclides in these reactions is associated with peripheral nucleon exchange. In such peripheral encounters, the neutron skins of the neutron-rich 124Sn and 64Ni target nuclei may play an important role. From a practical viewpoint, such reactions between massive neutron-rich nuclei offer a novel and attractive synthetic avenue to access extremely neutron-rich rare isotopes towards the neutron-drip line.Comment: 4 pages, 3 figures, submitted to Phys. Rev. Let

Nuclear rockets based on graphite reactor technology - Their applications and development status

Development and mission applications of nuclear rockets based on graphite reactor technolog

Dynamics and Instabilities of Planar Tensile Cracks in Heterogeneous Media

The dynamics of tensile crack fronts restricted to advance in a plane are studied. In an ideal linear elastic medium, a propagating mode along the crack front with a velocity slightly less than the Rayleigh wave velocity, is found to exist. But the dependence of the effective fracture toughness $\Gamma(v)$ on the crack velocity is shown to destabilize the crack front if $(d\Gamma)/(dv)<0$. Short wavelength radiation due to weak random heterogeneities leads to this instability at low velocities. The implications of these results for the crack dynamics are discussed.Comment: 12 page

Structural, item, and test generalizability of the psychopathology checklist - revised to offenders with intellectual disabilities

The Psychopathy Checklist–Revised (PCL-R) is the most widely used measure of psychopathy in forensic clinical practice, but the generalizability of the measure to offenders with intellectual disabilities (ID) has not been clearly established. This study examined the structural equivalence and scalar equivalence of the PCL-R in a sample of 185 male offenders with ID in forensic mental health settings, as compared with a sample of 1,212 male prisoners without ID. Three models of the PCL-R’s factor structure were evaluated with confirmatory factor analysis. The 3-factor hierarchical model of psychopathy was found to be a good fit to the ID PCL-R data, whereas neither the 4-factor model nor the traditional 2-factor model fitted. There were no cross-group differences in the factor structure, providing evidence of structural equivalence. However, item response theory analyses indicated metric differences in the ratings of psychopathy symptoms between the ID group and the comparison prisoner group. This finding has potential implications for the interpretation of PCL-R scores obtained with people with ID in forensic psychiatric settings

Analysis of the TSC1and TSC2genes in sporadic renal cell carcinomas

The genetic events involved in the aetiology of non-clear-cell renal cell carcinoma (RCC) and a proportion of clear cell RCC remain to be defined. Germline mutations of the TSC1and TSC2genes cause tuberous sclerosis (TSC), a multi-system hamartoma syndrome that is also associated with RCC. We assessed 17 sporadic clear cell RCCs with a previously identified VHLmutation, 15 clear-cell RCCs without an identified VHLmutation and 15 non-clear-cell RCCs for loss of heterozygosity (LOH) at chromosomes 9q34 and 16p13.3, the chromosomal locations of TSC1and TSC2. LOH was detected in 4/9, 1/11 and 3/13 cases informative at both loci. SSCP analysis of the whole coding region of the retained allele did not reveal any cases with a detectable intragenic second somatic mutation. Furthermore, RT-PCR analysis of TSC1and TSC2on total RNA from 8 clear-cell RCC cell lines confirmed expression of both TSC genes. These data indicate that biallelic inactivation of TSC1or TSC2is not frequent in sporadic RCC and suggests that the molecular mechanisms of renal carcinogenesis in TSC are likely to be distinct. © 2001 Cancer Research Campaignhttp://www.bjcancer.com  http://www.bjcancer.co