140 research outputs found

    Detection and Estimation Theory

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    Contains reports on two research projects.Joint Services Electronics Programs (U. S. Army, U. S. Navy, and U.S. Air Force)under Contract DA 28-043-AMC-02536(E

    Processing and Transmission of Information

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    Contains reports on four research projects.National Aeronautics and Space Administration (Grant NGL 22-009-013

    Processing and Transmission of Information

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    Contains research objectives, summary of research and reports on one research project.Joint Services Electronics Programs (U. S. Army, U.S. Navy, and U. S. Air Force) under Contract DA 28-043-AMC-02536(E)National Aeronautics and Space Administration (Grant NGL-22-009-013

    Processing and Transmission of Information

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    Contains research objectives and reports on three research projects.National Aeronautics and Space Administration (Grant NsG-334)Joint Services Electronics Programs (U. S. Army, U.S. Navy, and U.S. Air Force) under Contract DA 28-043-AMC-02536(E

    Interfacial Polymerization for Colorimetric Labeling of Protein Expression in Cells

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    Determining the location of rare proteins in cells typically requires the use of on-sample amplification. Antibody based recognition and enzymatic amplification is used to produce large amounts of visible label at the site of protein expression, but these techniques suffer from the presence of nonspecific reactivity in the biological sample and from poor spatial control over the label. Polymerization based amplification is a recently developed alternative means of creating an on-sample amplification for fluorescence applications, while not suffering from endogenous labels or loss of signal localization. This manuscript builds upon polymerization based amplification by developing a stable, archivable, and colorimetric mode of amplification termed Polymer Dye Labeling. The basic concept involves an interfacial polymer grown at the site of protein expression and subsequent staining of this polymer with an appropriate dye. The dyes Evans Blue and eosin were initially investigated for colorimetric response in a microarray setting, where both specifically stained polymer films on glass. The process was translated to the staining of protein expression in human dermal fibroblast cells, and Polymer Dye Labeling was specific to regions consistent with desired protein expression. The labeling is stable for over 200 days in ambient conditions and is also compatible with modern mounting medium

    Processing and Transmission of Information

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    Contains research objectives and reports on two research projects.National Aeronautics and Space Administration (Grant NsG-334)National Aeronautics and Space Administration (Grant NsG-496)Joint Services Electronics Programs (U. S. Army, U.S. Navy, and U.S. Air Force) under Contract DA 36-039-AMC-03200(E)National Science Foundation (Grant GP-835)Sloan Fund for Basic Research (M.I.T. Grant

    Processing and Transmission of Information

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    Contains research objectives, summary of research and reports on two research projects.National Aeronautics and Space Administration (Grant NGL 22-009-013)Joint Services Electronics Programs (U. S. Army, U. S. Navy, and U. S. Air Force) under Contract DA 28-043-AMC-02536(E)U. S. Army Research Office - Durham (Contract DAHCO4-69-C-0042

    Multi-Wavelength Properties of the Type IIb SN 2008ax

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    We present the UV, optical, X-ray, and radio properties of the Type IIb SN 2008ax discovered in NGC 4490. The observations in the UV are one of the earliest of a Type IIb supernova (SN). On approximately day four after the explosion, a dramatic upturn in the u and uvw1 (lambda_c = 2600 Angstroms) light curves occurred after an initial rapid decline which is attributed to adiabatic cooling after the initial shock breakout. This rapid decline and upturn is reminiscent of the Type IIb SN 1993J on day six after the explosion. Optical/near-IR spectra taken around the peak reveal prominent H-alpha, HeI, and CaII absorption lines. A fading X-ray source is also located at the position of SN 2008ax, implying an interaction of the SN shock with the surrounding circumstellar material and a mass-loss rate of the progenitor of M_dot = (9+/-3)x10^-6 solar masses per year. The unusual time evolution (14 days) of the 6 cm peak radio luminosity provides further evidence that the mass-loss rate is low. Combining the UV, optical, X-ray, and radio data with models of helium exploding stars implies the progenitor of SN 2008ax was an unmixed star in an interacting-binary. Modeling of the SN light curve suggests a kinetic energy (E_k) of 0.5x10^51 ergs, an ejecta mass (M_ej) of 2.9 solar masses, and a nickel mass (M_Ni) of 0.06 solar masses.Comment: Accepted to ApJ Letters, 14 pages, 3 figures, 2 table

    Processing and Transmission of Information

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    Contains research objectives and summary of research.National Aeronautics and Space Administration (Grant NGL 22-009-013)National Aeronautics and Space Administration (Contract NAS8-27733)Joint Services Electronics Programs (U. S. Army, U. S. Navy, and U. S. Air Force) under Contract DAAB07-71-C-0300U.S. Army Research Office - Durham (Contract DAHC04-71-C-0039)U.S. Army Research Office - Durham (Contract DAHC04-69-C-0042

    A Lyman-alpha-only AGN from the Sloan Digital Sky Survey

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    The Sloan Digital Sky Survey has discovered a z=2.4917 radio-loud active galactic nucleus (AGN) with a luminous, variable, low-polarization UV continuum, H I two-photon emission, and a moderately broad Lyman-alpha line (FWHM = 1430 km/s) but without obvious metal-line emission. SDSS J113658.36+024220.1 does have associated metal-line absorption in three distinct, narrow systems spanning a velocity range of 2710 km/s. Despite certain spectral similarities, SDSS J1136+0242 is not a Lyman-break galaxy. Instead, the Ly-alpha and two-photon emission can be attributed to an extended, low-metallicity narrow-line region. The unpolarized continuum argues that we see SDSS J1136+0242 very close to the axis of any ionization cone present. We can conceive of two plausible explanations for why we see a strong UV continuum but no broad-line emission in this `face-on radio galaxy' model for SDSS J1136+0242: the continuum could be relativistically beamed synchrotron emission which swamps the broad-line emission; or, more likely, SDSS J1136+0242 could be similar to PG 1407+265, a quasar in which for some unknown reason the high-ionization emission lines are very broad, very weak, and highly blueshifted.Comment: AJ, in press, 10 pages emulateapj forma
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