Fluid surface behavior in low gravity. Center discretionary fund no. 83-21

Measurements of rotating equilibrium bubble shapes in the low-gravity environment of a free-falling aircraft are presented. Emphasis is placed on bubbles which intersect the container boundaries. These data are compared with theoretical profiles derived from Laplace's formula and are in good agreement with the measurements. Two types of instability are explored. The first occurs when the baffle spacing is too large for the bubble to intersect both the top and bottom boundaries. The second occurs when the hydrostatic pressure beneath a displaced free surface does not compensate for pressure change due to capillary forces. The interface shape depends on the contact angle, the radius of intersection with container, and the parameter F which is a measure of the relative importance of centrifugal force to surface tension. For isolated bubbles, F has a maximum value of 1/2. A further increase in F causes the bubble to break contact with the axis of rotation. For large values of F, the bubble becomes more cylindrical and the capillary rise occurs over a thinner layer so that the small radius of curvature can generate enough pressure drop to balance the increased hydrostatic contribution

Growth or decline in the Church of England during the decade of Evangelism: did the Churchmanship of the Bishop matter?

The Decade of Evangelism occupied the attention of the Church of England throughout the 1990s. The present study employs the statistics routinely published by the Church of England in order to assess two matters: the extent to which these statistics suggest that the 43 individual dioceses finished the decade in a stronger or weaker position than they had entered it and the extent to which, according to these statistics, the performance of dioceses led by bishops shaped in the Evangelical tradition differed from the performance of dioceses led by bishops shaped in the Catholic tradition. The data demonstrated that the majority of dioceses were performing less effectively at the end of the decade than at the beginning, in terms of a range of membership statistics, and that the rate of decline varied considerably from one diocese to another. The only exception to the trend was provided by the diocese of London, which experienced some growth. The data also demonstrated that little depended on the churchmanship of the diocesan bishop in shaping diocesan outcomes on the performance indicators employed in the study

Social Dilemmas and Cooperation in Complex Networks

In this paper we extend the investigation of cooperation in some classical evolutionary games on populations were the network of interactions among individuals is of the scale-free type. We show that the update rule, the payoff computation and, to some extent the timing of the operations, have a marked influence on the transient dynamics and on the amount of cooperation that can be established at equilibrium. We also study the dynamical behavior of the populations and their evolutionary stability.Comment: 12 pages, 7 figures. to appea

A microfluidic processor for gene expression profiling of single human embryonic stem cells

The gene expression of human embryonic stem cells (hESC) is a critical aspect for understanding the normal and pathological development of human cells and tissues. Current bulk gene expression assays rely on RNA extracted from cell and tissue samples with various degree of cellular heterogeneity. These cell population averaging data are difficult to interpret, especially for the purpose of understanding the regulatory relationship of genes in the earliest phases of development and differentiation of individual cells. Here, we report a microfluidic approach that can extract total mRNA from individual single-cells and synthesize cDNA on the same device with high mRNA-to-cDNA efficiency. This feature makes large-scale single-cell gene expression profiling possible. Using this microfluidic device, we measured the absolute numbers of mRNA molecules of three genes (B2M, Nodal and Fzd4) in a single hESC. Our results indicate that gene expression data measured from cDNA of a cell population is not a good representation of the expression levels in individual single cells. Within the G0/G1 phase pluripotent hESC population, some individual cells did not express all of the 3 interrogated genes in detectable levels. Consequently, the relative expression levels, which are broadly used in gene expression studies, are very different between measurements from population cDNA and single-cell cDNA. The results underscore the importance of discrete single-cell analysis, and the advantages of a microfluidic approach in stem cell gene expression studies

A Flattened Protostellar Envelope in Absorption around L1157

Deep Spitzer IRAC images of L1157 reveal many of the details of the outflow and the circumstellar environment of this Class 0 protostar. In IRAC band 4, 8 microns, there is a flattened structure seen in absorption against the background emission. The structure is perpendicular to the outflow and is extended to a diameter of 2 arcminutes. This structure is the first clear detection of a flattened circumstellar envelope or pseudo-disk around a Class 0 protostar. Such a flattened morphology is an expected outcome for many collapse theories that include magnetic fields or rotation. We construct an extinction model for a power-law density profile, but we do not constrain the density power-law index.Comment: ApJL accepte

Multiwell plates for complementation tests of Fusarium.

Vegetative compatibility group (VCG) is a phenotype used to subdivide and categorize populations of Fusarium species (Correll et al. 1987 Phytopathology 77:1640-1646)

Mapping the cellular electrophysiology of rat sympathetic preganglionic neurones to their roles in cardiorespiratory reflex integration:A whole cell recording study in situ

Sympathetic preganglionic neurones (SPNs) convey sympathetic activity flowing from the CNS to the periphery to reach the target organs. Although previous in vivo and in vitro cell recording studies have explored their electrophysiological characteristics, it has not been possible to relate these characteristics to their roles in cardiorespiratory reflex integration. We used the working heart–brainstem preparation to make whole cell patch clamp recordings from T3–4 SPNs (n = 98). These SPNs were classified by their distinct responses to activation of the peripheral chemoreflex, diving response and arterial baroreflex, allowing the discrimination of muscle vasoconstrictor-like (MVC(like), 39%) from cutaneous vasoconstrictor-like (CVC(like), 28%) SPNs. The MVC(like) SPNs have higher baseline firing frequencies (2.52 ± 0.33 Hz vs. CVC(like) 1.34 ± 0.17 Hz, P = 0.007). The CVC(like) have longer after-hyperpolarisations (314 ± 36 ms vs. MVC(like) 191 ± 13 ms, P < 0.001) and lower input resistance (346 ± 49  MΩ vs. MVC(like) 496 ± 41 MΩ, P < 0.05). MVC(like) firing was respiratory-modulated with peak discharge in the late inspiratory/early expiratory phase and this activity was generated by both a tonic and respiratory-modulated barrage of synaptic events that were blocked by intrathecal kynurenate. In contrast, the activity of CVC(like) SPNs was underpinned by rhythmical membrane potential oscillations suggestive of gap junctional coupling. Thus, we have related the intrinsic electrophysiological properties of two classes of SPNs in situ to their roles in cardiorespiratory reflex integration and have shown that they deploy different cellular mechanisms that are likely to influence how they integrate and shape the distinctive sympathetic outputs

Global Solution to the Three-Dimensional Incompressible Flow of Liquid Crystals

The equations for the three-dimensional incompressible flow of liquid crystals are considered in a smooth bounded domain. The existence and uniqueness of the global strong solution with small initial data are established. It is also proved that when the strong solution exists, all the global weak solutions constructed in [16] must be equal to the unique strong solution

Aspirin desensitization in patients undergoing percutaneous coronary intervention: A survey of current practice

Background: Aspirin remains the mainstay of anti-platelet therapy in cardiac patients.However, if a patient is allergic to aspirin and dual anti-platelet therapy is indicated — suchas with percutaneous coronary intervention (PCI), then there is no clear guidance. Onepossibility is aspirin desensitization. A variety of protocols exist for the rapid desensitization ofpatients with aspirin allergy. The aim of this survey was to assess current knowledge andpractice regarding aspirin desensitization in the UK.Methods and results: We conducted a UK wide survey of all UK 116 PCI centers andobtained complete responses from 40 (35.4%) centers. Of these, just 7 (17.5%) centers hadpreviously desensitised patients; 29 (87.9%) centers suggested a lack of a local protocolprevented them from desensitizing, with 10 (30.3%) unsure of how to conduct desensitization.Only 5 (12.5%) centers had a local policy for aspirin desensitization although 25 (64.1%)units had a clinical strategy for dealing with aspirin allergy; the majority (72%) giving higherdoses of thienopyridine class drugs.Conclusions: In the UK, there appears to be no consistent approach to patients with aspirinallergy. Patients undergoing PCI benefit from dual anti-platelet therapy (including aspirin),and aspirin desensitization in those with known allergy may facilitate this. Sustained effortshould be placed on encouraging UK centers to use desensitization as a treatment modalityprior to PCI rather than avoiding aspirin altogether