Direct measurements of the energy flux due to chemical reactions at the surface of a silicon sample interacting with a SF6 plasma

Energy exchanges due to chemical reactions between a silicon surface and a SF6 plasma were directly measured using a heat flux microsensor (HFM). The energy flux evolution was compared with those obtained when only few reactions occur at the surface to show the part of chemical reactions. At 800 W, the measured energy flux due to chemical reactions is estimated at about 7 W.cm\^{-2} against 0.4 W.cm\^{-2} for ion bombardment and other contributions. Time evolution of the HFM signal is also studied. The molar enthalpy of the reaction giving SiF4 molecules was evaluated and is consistent with values given in literature.Comment: 3 page

Long term dynamics of two populations of Prosopis caldenia Burkart

Los cambios más importantes registrados en la distribución del caldén (Prosopis caldenia Burkart) en la región semiárida central de Argentina, Incluyen el aumento de la densidad de esta especie y la invasión de pastizales naturales. Los factores causales y la dinámica de estos cambios no han sido detalladamente estudiados. En este trabajo se relaciona la dinámica de dos poblaciones de caldén con factores tales como manejo, fuego y precipitaciones. Se eligieron como áreas de estudios una planicie cuya vegetación original era un pastizal (sitio 1) y un bosque de caldén que originalmente fue una sabana (sitio 2). En cada sitio se determinaron los patrones espaciales en base a transectas y las tasas de Implantación las cuales fueron inferidas por dendroecologia. Las densidades estimadas fueron 586 y 1259 plantas/ha, para el sitio 1 y 2 respectivamente. No se encontraron evidencias de agrupamiento para la escala espacial de este estudio (p=0.52, sitio 1 y p=0.08, sitio 2 para n=112). Las edades de los individuos muestreados comprendieron un rango de 3 a 65 años en el sitio 1 y de 8 a 55 años en el sitio 2 (en este sitio 2 solo fueron muestreadas las plantas con diámetro menor de 30 cm). Primariamente los dos sitios fueron utilizados para erra de ovinos; durante ese periodo, las tasas de establecimiento fueron 0.99 plantas/ha/año (16 años) en el sitio 1, Y 10 plantas/ha/año (15 años) en el sitio 2. Posteriormente luego de diez años desde la introducción del ganado vacuno, las tasas ascendieron a 12.7 y 48.5 plantas/ha/año respectivamente. Un evento de fuego fue registrado en cada sitio (1980 en el sitio 1 y 1964 en el sitio 2) pero este factor no alteró la tendencia ascendente de la densidad. En el sitio 2 el Incendio fue contemporáneo con la introducción del ganado y coincide a su vez con un importante incremento en el establecimiento. No se encontró relación entre la dinámica de la población de caldén y las fluctuaciones en la precipitación anual o estacional. Las variaciones registradas en las tasas de establecimiento coincidieron con cambios en el manejo ganadero, confirmando la Importancia del ganado en la dispersión de las semillas de caldén.Resúmenes de Trabajos presentados en otras publicaciones (por docentes de la U.N.L.Pam.) Publicado en JOURNAL OF RANGE MANAGEMENT, 51 (6), November 199

Genome-based discovery of polyketide-derived secondary metabolism pathways in the barley pathogen <i>Ramularia collo-cygni</i>

Ramularia collo-cygni causes Ramularia leaf spot (RLS) disease of barley. The fungus develops asymptomatically within its host until late in the growing season, when necrotic lesions become visible on upper leaves. Fungal secondary metabolites (SM) have been proposed as important factors in RLS lesion formation but the biosynthetic pathways involved remain largely unknown. Mining the R. collo-cygni genome revealed the presence of 10 polyketide synthases (PKS), 10 nonribosomal peptide synthetases (NRPS), and 3 hybrid PKS-NRPS (HPS) identified within clusters of genes with predicted functions associated with secondary metabolism. SM core genes along with their predicted transcriptional regulators exhibited transcriptional coexpression during infection of barley plants. Moreover, their expression peaked during early stages of host colonization and preceded or overlapped with the appearance of disease symptoms, suggesting that SM may manipulate the host to promote colonization or protect R. collo-cygni from competing organisms. Accordingly, R. collo-cygni inhibited the growth of several fungi in vitro, indicating that it synthesized and excreted antifungal agents. Taken together, these findings demonstrate that the R. collo-cygni genome contains the genetic architecture to synthesize a wide range of SM and suggests that coexpression of PKS and HPS is associated with competitive colonization of the host and early symptom development. [Formula: see text] Copyright © 2018 The Author(s). This is an open access article distributed under the CC BY 4.0 International license . </jats:p

Inventory of molecular markers affecting biological characteristics of avian influenza A viruses

Avian influenza viruses (AIVs) circulate globally, spilling over into domestic poultry and causing zoonotic infections in humans. Fortunately, AIVs are not yet capable of causing sustained human-to-human infection; however, AIVs are still a high risk as future pandemic strains, especially if they acquire further mutations that facilitate human infection and/or increase pathogenesis. Molecular characterization of sequencing data for known genetic markers associated with AIV adaptation, transmission, and antiviral resistance allows for fast, efficient assessment of AIV risk. Here we summarize and update the current knowledge on experimentally verified molecular markers involved in AIV pathogenicity, receptor binding, replicative capacity, and transmission in both poultry and mammals with a broad focus to include data available on other AIV subtypes outside of A/H5N1 and A/H7N9

Ignition and extinction phenomena in helium micro hollow cathode discharges

International audienceMicro hollow cathode discharges (MHCD) were produced using 250 µm thick dielectric layer of alumina sandwiched between two nickel electrodes of 8 µm thickness. A through cavity at the center of the chip was formed by laser drilling technique. MHCD with a diameter of few hundreds of micrometers allowed us to generate direct current discharges in helium at up to atmospheric pressure. A slowly varying ramped voltage generator was used to study the ignition and the extinction periods of the microdischarges. The analysis was performed by using electrical characterisation of the V-I behaviour and the measurement of He*(3S1) metastable atoms density by tunable diode laser spectroscopy. At the ignition of the microdischarges, 2 µs long current peak as high as 24 mA was observed, sometimes followed by low amplitude damped oscillations. At helium pressure above 400 Torr, an oscillatory behaviour of the discharge current was observed just before the extinction of the microdischarges. The same type of instability in the extinction period at high pressure also appeared on the density of He*(3S1) metastable atoms, but delayed by a few µs relative to the current oscillations. Metastable atoms thus cannot be at the origin of the generation of the observed instabilities

Surface electrons at plasma walls

In this chapter we introduce a microscopic modelling of the surplus electrons on the plasma wall which complements the classical description of the plasma sheath. First we introduce a model for the electron surface layer to study the quasistationary electron distribution and the potential at an unbiased plasma wall. Then we calculate sticking coefficients and desorption times for electron trapping in the image states. Finally we study how surplus electrons affect light scattering and how charge signatures offer the possibility of a novel charge measurement for dust grains.Comment: To appear in Complex Plasmas: Scientific Challenges and Technological Opportunities, Editors: M. Bonitz, K. Becker, J. Lopez and H. Thomse

Value of syndromic surveillance within the Armed Forces for early warning during a dengue fever outbreak in French Guiana in 2006

<p>Abstract</p> <p>Background</p> <p>A dengue fever outbreak occured in French Guiana in 2006. The objectives were to study the value of a syndromic surveillance system set up within the armed forces, compared to the traditional clinical surveillance system during this outbreak, to highlight issues involved in comparing military and civilian surveillance systems and to discuss the interest of syndromic surveillance for public health response.</p> <p>Methods</p> <p>Military syndromic surveillance allows the surveillance of suspected dengue fever cases among the 3,000 armed forces personnel. Within the same population, clinical surveillance uses several definition criteria for dengue fever cases, depending on the epidemiological situation. Civilian laboratory surveillance allows the surveillance of biologically confirmed cases, within the 200,000 inhabitants.</p> <p>Results</p> <p>It was shown that syndromic surveillance detected the dengue fever outbreak several weeks before clinical surveillance, allowing quick and effective enhancement of vector control within the armed forces. Syndromic surveillance was also found to have detected the outbreak before civilian laboratory surveillance.</p> <p>Conclusion</p> <p>Military syndromic surveillance allowed an early warning for this outbreak to be issued, enabling a quicker public health response by the armed forces. Civilian surveillance system has since introduced syndromic surveillance as part of its surveillance strategy. This should enable quicker public health responses in the future.</p

A DNA-barcode biodiversity standard analysis method (DNA-BSAM) reveals a large variance in the effect of a range of biological, chemical and physical soil management interventions at different sites, but location is one of the most important aspects determining the nature of agricultural soil microbiology

There are significant knowledge gaps in our understanding of how to sustainably manage agricultural soils to preserve soil biodiversity. Here we evaluate and quantify the effects of agricultural management and location on soil microbiology using nine field trials that have consistently applied different soil management practices in the United Kingdom using DNA barcode sequence data. We tested the basic hypothesis that various agricultural management interventions have a significant and greater effect on soil bacterial and fungal diversity than geographic location. The analyses of soil microbial DNA sequence data to date has lacked standardisation which prevents meaningful comparisons across sites and studies. Therefore, to analyse these data and crucially compare and quantify the size of any effects on soil bacterial and fungal biodiversity between sites, we developed and employed a post-sequencing DNA-barcode biodiversity standard analysis method (DNA-BSAM). The DNA-BSAM comprises a series of standardised bioinformatic steps for processing sequences but more importantly defines a standardised set of ecological indices and statistical tests. Use of the DNA-BSAM reveals the hypothesis was not strongly supported, and this was primarily because: 1) there was a large variance in the effects of various management interventions at different sites, and 2) that location had an equivalent or greater effect size than most management interventions for most metrics. Some dispersed sites imposed the same organic amendments interventions but showed different responses, and this combined with observations of strong differences in soil microbiomes by location tentatively suggests that any effect of management may be contingent on location. This means it could be unreliable to extrapolate the findings of individual trials to others. The widespread use of a standard approach will allow meaningful cross-comparisons between soil microbiome studies and thus a substantial evidence-base of the effects of land-use on soil microbiology to accumulate and inform soil management decisions.Agriculture and Horticulture Development Board (AHDB); British Beet Research Organisation (BBRO