Human P2Y11 Expression Level Affects Human P2X7 Receptor-Mediated Cell Death

Adenosine triphosphate (ATP) is known to induce cell death in T lymphocytes at high extracellular concentrations. CD4+ and CD8+ T lymphocytes have a differential response to ATP, which in mice is due to differences in the P2X7 receptor expression levels. By contrast, we observed that the difference in human CD4+ and CD8+ T lymphocyte response toward the synthetic ATP-analog BzATP is not explained by a difference in human P2X7 receptor expression. Rather, the BzATP-induced human P2X7 receptor response in naïve and immune-activated lymphocyte subtypes correlated with the expression of another ATP-binding receptor: the human P2Y11 receptor. In a recombinant expression system, the coexpression of the human P2Y11 receptor counteracted BzATP-induced human P2X7 receptor-driven lactate dehydrogenase release (a marker of cell death) and pore formation independent of calcium signaling. A mutated non-signaling human P2Y11 receptor had a similar human P2X7 receptor-inhibitory effect on pore formation, thus demonstrating that the human P2X7 receptor interference was not caused by human P2Y11 receptor signaling. In conclusion, we demonstrate an important species difference in the ATP-mediated cell death between mice and human cells and show that in human T lymphocytes, the expression of the human P2Y11 receptor correlates with human P2X7 receptor-driven cell death following BzATP stimulation

sj-pdf-1-mrj-10.1177_00222437221112058 - Supplemental material for Role Integration Increases the Fungibility of Mentally Accounted Funds

Supplemental material, sj-pdf-1-mrj-10.1177_00222437221112058 for Role Integration Increases the Fungibility of Mentally Accounted Funds by Iman Paul, Jeffrey R. Parker and Sara Loughran Dommer in Journal of Marketing Research</p

Vorrichtung und Verfahren zum Erwaermen von Bauteilen aus mikrowellenabsorbierendem Kunststoff

EP 991136 A UPAB: 20000508 NOVELTY - A heater for plastics comprises a microwave generator, a waveguide and screened antenna, and has a safe and coaxial wave trap which is completely open for insertion of e.g. a plastic tube end, for concentrated, controlled, efficient local heating. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for the method of heating components made of microwave-absorbing plastic. Preferred features: The screen projection beyond the antenna tip, is a quarter wavelength or more. The zone between the antenna tip and the screen is dimensioned in accordance with the size of the component to be heated. In one region it is especially narrow, to concentrate heating locally. The component inserted in the narrow region is heated there, in accordance with specific requirements. Between the antenna and screen, dielectric is included. The antenna tip is e.g. conical, a mandrel, a rod or a hook. Antenna tip and screen are coaxial. In the method, components are heated, to weld them together. A double version of the heater (illustrated in the disclosure) may be constructed to heat e.g. two tube ends simultaneously, to weld them together. Heating may precede permanent deformation. A conically-tipped antenna may be used for tube end flaring. Heating may induce crosslinking. USE - To heat plastic components, especially tube ends, using microwaves. ADVANTAGE - Despite being very compact for the duty, the head heats particularly uniformly. It is very simple to use. A striking feature is the containment of microwave radiation, a consequence of selecting dimensions in relation to the wavelength. Usefully, the screen is wide open at the end, yet blocks microwave release. The limited heating region ensures efficient use of energy; the microwave field may be concentrated for more intense local heating. Heating and deformation of pipe ends, suggests application as a pipeline joint welding machine

Changing pattern of brain hemorrhage during 12 years of computed axial tomography

We examined whether the pattern of cerebral hemorrhage changed after the introduction of computed tomography

image_2_Human P2Y11 Expression Level Affects Human P2X7 Receptor-Mediated Cell Death.tif

<p>Adenosine triphosphate (ATP) is known to induce cell death in T lymphocytes at high extracellular concentrations. CD4⁺ and CD8⁺ T lymphocytes have a differential response to ATP, which in mice is due to differences in the P2X7 receptor expression levels. By contrast, we observed that the difference in human CD4⁺ and CD8⁺ T lymphocyte response toward the synthetic ATP-analog BzATP is not explained by a difference in human P2X7 receptor expression. Rather, the BzATP-induced human P2X7 receptor response in naïve and immune-activated lymphocyte subtypes correlated with the expression of another ATP-binding receptor: the human P2Y₁₁ receptor. In a recombinant expression system, the coexpression of the human P2Y₁₁ receptor counteracted BzATP-induced human P2X7 receptor-driven lactate dehydrogenase release (a marker of cell death) and pore formation independent of calcium signaling. A mutated non-signaling human P2Y₁₁ receptor had a similar human P2X7 receptor-inhibitory effect on pore formation, thus demonstrating that the human P2X7 receptor interference was not caused by human P2Y₁₁ receptor signaling. In conclusion, we demonstrate an important species difference in the ATP-mediated cell death between mice and human cells and show that in human T lymphocytes, the expression of the human P2Y₁₁ receptor correlates with human P2X7 receptor-driven cell death following BzATP stimulation.</p