Microbial metagenome-assembled genomes of the Fram Strait from short and long read sequencing platforms

The impacts of climate change on the Arctic Ocean are manifesting throughout the ecosystem at an unprecedented rate. Of global importance are the impacts on heat and freshwater exchange between the Arctic and North Atlantic Oceans. An expanding Atlantic influence in the Arctic has accelerated sea-ice decline, weakened water column stability and supported the northward shift of temperate species. The only deep-water gateway connecting the Arctic and North Atlantic and thus, fundamental for these exchange processes is the Fram Strait. Previous research in this region is extensive, however, data on the ecology of microbial communities is limited, reflecting the wider bias towards temperate and tropical latitudes. Therefore, we present 14 metagenomes, 11 short-read from Illumina and three long-read from PacBio Sequel II, of the 0.2–3 µm fraction to help alleviate such biases and support future analyses on changing ecological patterns. Additionally, we provide 136 species-representative, manually refined metagenome-assembled genomes which can be used for comparative genomics analyses and addressing questions regarding functionality or distribution of taxa

Molecular Gas, Dust and Star Formation in Galaxies: II. Dust properties and scalings in \sim\ 1600 nearby galaxies

We aim to characterize the relationship between dust properties. We also aim to provide equations to estimate accurate dust properties from limited observational datasets. We assemble a sample of 1,630 nearby (z<0.1) galaxies-over a large range of Mstar, SFR - with multi-wavelength observations available from wise, iras, planck and/or SCUBA. The characterization of dust emission comes from SED fitting using Draine & Li dust models, which we parametrize using two components (warm and cold ). The subsample of these galaxies with global measurements of CO and/or HI are used to explore the molecular and/or atomic gas content of the galaxies. The total Lir, Mdust and dust temperature of the cold component (Tc) form a plane that we refer to as the dust plane. A galaxy's sSFR drives its position on the dust plane: starburst galaxies show higher Lir, Mdust and Tc compared to Main Sequence and passive galaxies. Starburst galaxies also show higher specific Mdust (Mdust/Mstar) and specific Mgas (Mgas/Mstar). The Mdust is more closely correlated with the total Mgas (atomic plus molecular) than with the individual components. Our multi wavelength data allows us to define several equations to estimate Lir, Mdust and Tc from one or two monochromatic luminosities in the infrared and/or sub-millimeter. We estimate the dust mass and infrared luminosity from a single monochromatic luminosity within the R-J tail of the dust emission, with errors of 0.12 and 0.20dex, respectively. These errors are reduced to 0.05 and 0.10 dex, respectively, if the Tc is used. The Mdust is correlated with the total Mism (Mism \propto Mdust^0.7). For galaxies with Mstar 8.5<log(Mstar/Msun) < 11.9, the conversion factor \alpha_850mum shows a large scatter (rms=0.29dex). The SF mode of a galaxy shows a correlation with both the Mgass and Mdust: high Mdust/Mstar galaxies are gas-rich and show the highest SFRs.Comment: 24 pages, 28 figures, 6 tables, Accepted for publication in A&

A mesoscopic ring as a XNOR gate: An exact result

We describe XNOR gate response in a mesoscopic ring threaded by a magnetic flux $\phi$. The ring is attached symmetrically to two semi-infinite one-dimensional metallic electrodes and two gate voltages, viz, $V_a$ and $V_b$, are applied in one arm of the ring which are treated as the inputs of the XNOR gate. The calculations are based on the tight-binding model and the Green's function method, which numerically compute the conductance-energy and current-voltage characteristics as functions of the ring-to-electrode coupling strength, magnetic flux and gate voltages. Our theoretical study shows that, for a particular value of $\phi$ ($=\phi_0/2$) ($\phi_0=ch/e$, the elementary flux-quantum), a high output current (1) (in the logical sense) appears if both the two inputs to the gate are the same, while if one but not both inputs are high (1), a low output current (0) results. It clearly exhibits the XNOR gate behavior and this aspect may be utilized in designing an electronic logic gate.Comment: 8 pages, 5 figure

Time Dependent Current Oscillations Through a Quantum Dot

Time dependent phenomena associated to charge transport along a quantum dot in the charge quantization regime is studied. Superimposed to the Coulomb blockade behaviour the current has novel non-linear properties. Together with static multistabilities in the negative resistance region of the I-V characteristic curve, strong correlations at the dot give rise to self-sustained current and charge oscillations. Their properties depend upon the parameters of the quantum dot and the external applied voltages.Comment: 4 pages, 3 figures; to appear in PR

Dynamic instabilities in resonant tunneling induced by a magnetic field

We show that the addition of a magnetic field parallel to the current induces self sustained intrinsic current oscillations in an asymmetric double barrier structure. The oscillations are attributed to the nonlinear dynamic coupling of the current to the charge trapped in the well, and the effect of the external field over the local density of states across the system. Our results show that the system bifurcates as the field is increased, and may transit to chaos at large enough fields.Comment: 4 pages, 3 figures, accepted in Phys. Rev. Letter

Verrucomicrobiota are specialist consumers of sulfated methyl pentoses during diatom blooms

Marine algae annually sequester petagrams of carbon dioxide into polysaccharides, which are a central metabolic fuel for marine carbon cycling. Diatom microalgae produce sulfated polysaccharides containing methyl pentoses that are challenging to degrade for bacteria compared to other monomers, implicating these sugars as a potential carbon sink. Free-living bacteria occurring in phytoplankton blooms that specialise on consuming microalgal sugars, containing fucose and rhamnose remain unknown. Here, genomic and proteomic data indicate that small, coccoid, free-living Verrucomicrobiota specialise in fucose and rhamnose consumption during spring algal blooms in the North Sea. Verrucomicrobiota cell abundance was coupled with the algae bloom onset and accounted for up to 8% of the bacterioplankton. Glycoside hydrolases, sulfatases, and bacterial microcompartments, critical proteins for the consumption of fucosylated and sulfated polysaccharides, were actively expressed during consecutive spring bloom events. These specialised pathways were assigned to novel and discrete candidate species of the Akkermansiaceae and Puniceicoccaceae families, which we here describe as Candidatus Mariakkermansia forsetii and Candidatus Fucivorax forsetii. Moreover, our results suggest specialised metabolic pathways could determine the fate of complex polysaccharides consumed during algae blooms. Thus the sequestration of phytoplankton organic matter via methyl pentose sugars likely depend on the activity of specialised Verrucomicrobiota populations

GS305+04-26:Revisiting the ISM around the CenOB1 stellar association

Massive stars deeply modify their surrounding ISM via their high throughput of ionizing photons and their strong stellar winds. In this way they may create large expanding structures of neutral gas. We study a new large HI shell, labelled GS305+04-26, and its relationship with the OB association CenOB1. To carry out this study we have used a multi-wavelenght approach. We analyze neutral hydrogen (HI) line data retrieved from the Leiden-Argentina-Bonn (LAB) survey, new spectroscopic optical observations obtained at CASLEO, and make use of proper motion databases available via Internet. The analysis of the HI data reveals a large expanding structure GS305+04-26 centered at (l,b)=(305^{\degr}, +4^{\degr}) in the velocity range from -33 to -17 km/s. Based on its central velocity, -26 km/s, and using standard galactic rotation models, a distance of 2.5(+-)0.9 kpc is inferred. This structure, elliptical in shape, has major and minor axis of 440 and 270 pc, respectively. Its expansion velocity, total gaseous mass, and kinetic energy are ~8 km/s, (2.4(+-)0.5)x10^5 Mo, and (1.6(+-)0.4)x10^{50} erg, respectively. Several stars of the OB-association CenOB1 are seen projected onto, and within, the boundaries of GS305+04-26. Based on an analysis of proper motions, new members of CenOB1 are identified. The mechanical energy injected by these stars could have been the origin of this HI structure.Comment: 14 pages, 6 figures, A&A (in press

Niche differentiation among annually recurrent coastal marine group II Euryarchaeota

Since the discovery of archaeoplankton in 1992, the euryarchaeotal Marine Group II (MGII) remains uncultured and less understood than other planktonic archaea. We characterized the seasonal dynamics of MGII populations in the southern North Sea on a genomic and microscopic level over the course of four years. We recovered 34 metagenome-assembled genomes (MAGs) of MGIIa and MGIIb that corroborated proteorhodopsin-based photoheterotrophic lifestyles. However, MGIIa and MGIIb MAG genome sizes differed considerably (~1.9 vs. ~1.4 Mbp), as did their transporter, peptidase, flagella and sulfate assimilation gene repertoires. MGIIb populations were characteristic of winter samples, whereas MGIIa accounted for up to 23% of the community at the beginning of summer. Both clades consisted of annually recurring, sequence-discrete populations with low intra-population sequence diversity. Oligotyping of filtered cell-size fractions and microscopy consistently suggested that MGII cells were predominantly free-living. Cells were coccoid and ~0.7 µm in diameter, likely resulting in grazing avoidance. Based on multiple lines of evidence, we propose distinct niche adaptations of MGIIa and MGIIb Euryarchaeota populations that are characteristic of summer and winter conditions in the coastal North Sea

Aharonov-Bohm Effect for Parallel and T-shaped Double Quantum Dots

We investigate the Aharonov-Bohm (AB) effect for the double quantum dots in the Kondo regime using the slave-boson mean-field approximation. In contrast to the non-interacting case, where the AB oscillation generally has the period of 4$\pi$ when the two-subring structure is formed via the interdot tunneling $t_c$, we find that the AB oscillation has the period of 2$\pi$ in the Kondo regime. Such effects appear for the double quantum dots close to the T-shaped geometry even in the charge-fluctuation regime. These results follow from the fact that the Kondo resonance is always fixed to the Fermi level irrespective of the detailed structure of the bare dot-levels.Comment: 3 pages, 4 figures; minor change