‘Mild mannered’? Protest and mobilisation in Portugal under austerity, 2010–2013

Anti-austerity mobilisations in Southern Europe since 2010 have been widely debated in recent times. Commentators have emphasised the emergence of new political subjects such as the ‘precariat’ organised into loose, IT-connected movements. To what extent do these portrayals reflect the underlying dynamics of this protest cycle, and how do these movements interact with traditional political actors? Using Portugal as a case study, this article maps the cycle of anti-austerity contention between 2010 and 2013 to reveal a more complex picture, where traditional actors, including labour unions and left-wing political parties, emerge as key actors, facilitating and sustaining the discontinuous mobilisation of new forms of activism, while seeking to gain access to new constituencies through them.info:eu-repo/semantics/acceptedVersio

Scale-dependent maximum reinforcement percentage in reinforced concrete beams

The Cohesive/Overlapping Crack Model is able to describe the transition between cracking and crushing failures occurring in reinforced concrete beams by increasing beam depth and/or steel percentage. Within this Nonlinear Fracture Mechanics model, the tensile and compressive ultimate behaviors of the concrete matrix are modeled through two different process zones that advance independently one of another. Moreover, this model is able to investigate local mechanical instabilities occurring in the structural behavior of reinforced concrete structures: tensile snap-back and snap-through, which are due to concrete cracking or steel fracture, and the compressive snap-back occurring at the end of the plastic plateau, which is generated by the unstable growth of the crushing zone. In this context, the application of the Cohesive/Overlapping Crack Model highlights that the ductility, which is represented by the plastic rotation capacity of a reinforced concrete element subjected to bending, decreases as reinforcement percentage and/or beam depth increase. Thus, a scale-dependent maximum reinforcement percentage beyond which concrete crushing occurs prior to steel yielding is demonstrated to exist. In particular, the maximum steel percentage results to be inversely proportional to h0.25, h being the beam depth. In this way, a rational and quantitative definition of over-reinforcement is provided as a steel percentage depending on the beam depth

Circulating skeletal muscle related microRNAs profile in Piedmontese cattle during different age

Piedmontese cattle is known for double-muscle phenotype. MicroRNAs (miRNAs) play important role as regulators in skeletal muscle physiological processes, and we hypothesize that plasma miRNAs expression profiles could be affected by skeletal muscle growth status related to age. Plasma samples of cattle were collected during four different ages from first week of life until the time of commercial end of the fattening period before slaughter. Small-RNA sequencing data analysis revealed the presence of 40% of muscle-related miRNAs among the top 25 highly expressed miRNAs and, 19 miRNAs showed differential expression too. Using qRT-PCR, we validated in a larger bovine population, miRNAs involved in skeletal muscle physiology pathways. Comparing new-born with the other age groups, miR-10b, miR-126-5p, miR-143 and miR-146b were significantly up-regulated, whereas miR-21-5p, miR-221, miR-223 and miR-30b-5p were significantly down-regulated. High expression levels of miR-23a in all the groups were found. Myostatin, a negative regulator of skeletal muscle hypertrophy, was predicted as the target gene for miR-23a and miR-126-5p and we demonstrated their direct binding. Correlation analysis revealed association between miRNAs expression profiles and animals’ weights along the age. Circulating miRNAs could be promising for future studies on their biomarker potentialities to beef cattle selection

Circulating skeletal muscle related microRNAs profile in Piedmontese cattle during different age

Abstract Piedmontese cattle is known for double-muscle phenotype. MicroRNAs (miRNAs) play important role as regulators in skeletal muscle physiological processes, and we hypothesize that plasma miRNAs expression profiles could be affected by skeletal muscle growth status related to age. Plasma samples of cattle were collected during four different ages from first week of life until the time of commercial end of the fattening period before slaughter. Small-RNA sequencing data analysis revealed the presence of 40% of muscle-related miRNAs among the top 25 highly expressed miRNAs and, 19 miRNAs showed differential expression too. Using qRT-PCR, we validated in a larger bovine population, miRNAs involved in skeletal muscle physiology pathways. Comparing new-born with the other age groups, miR-10b, miR-126-5p, miR-143 and miR-146b were significantly up-regulated, whereas miR-21-5p, miR-221, miR-223 and miR-30b-5p were significantly down-regulated. High expression levels of miR-23a in all the groups were found. Myostatin, a negative regulator of skeletal muscle hypertrophy, was predicted as the target gene for miR-23a and miR-126-5p and we demonstrated their direct binding. Correlation analysis revealed association between miRNAs expression profiles and animals’ weights along the age. Circulating miRNAs could be promising for future studies on their biomarker potentialities to beef cattle selection

'Mild mannered'? Protest and mobilization in Portugal under austerity, 2010-2013

Em tempos recentes tem-se debatido muito as mobilizações anti-austeridade ocorridas na Europa do Sul desde 2010. Os comentadores frisaram a emergência de novos atores políticos, tais como o «precariado», organizados em movimentos pouco estruturados com base nas tecnologias de informação e comunicação. Até que ponto é que estes movimentos refletem a dinâmica subjacente deste ciclo de protestos e como é que interagem com os atores políticos tradicionais? Utilizando Portugal como um caso-estudo, este artigo traça o mapa das ações políticas reivindicativas anti-austeridade entre os anos de 2010 e 2013, revelando uma panorâmica mais complexa, onde os atores tradicionais, incluindo os sindicatos e os partidos políticos de esquerda, emergem como atores-chave, facilitando e nutrindo a mobilização intermitente de novas formas de ativismo, procurando ao mesmo tempo ganhar com elas acesso a novas clientelas eleitorais.info:eu-repo/semantics/publishedVersio

A low-cost portable vocal analyser for long-term monitoring and clinical investigation

A low-cost portable device has been developed at Politecnico di Torino to provide traceable measurements of vocal parameters during long-term monitoring as well as short ambulatory tests. The device, named Voice Care, is based on a contact microphone that is attached to the jugular notch of the subject under monitoring and on a wearable data acquisition unit that stores the raw samples of the signal generated by the vocal folds’ vibration. Post processing algorithms have been assessed to evaluate the vocal effort and the vocal load that voice professionals are subjected to during their daily activity, estimating the parameters sound pressure level, fundamental frequency and phonation time percentage. Other investigations are related to the length of voiced and unvoiced frames, whose distribution are dependent on the acoustic characteristics of the environment where the voice monitoring takes place. Another application of the Voice Care is related to short-term ambulatory tests, which allows the cooperation with physicians to make the device a reliable diagnostic tool. Processing algorithms have been extended to estimate other parameters, such as jitter, shimmer and voice quality indexes, that allow the phonatory status of the subject under monitoring to be evaluated. An experimental campaign has been performed involving thirty teachers in four primary schools who have been monitored for two to four days across one week of teaching. The effectiveness of the proposed device has been shown by the obtained results, which were in good agreement with the subjective impression and the classroom acoustics. Other specific tests have been performed in very different acoustic environments (anechoic, reverberant and semi- reverberant chambers) to highlight the device capability in evaluating the environment effects on the vocal production. Ambulatory tests for the optimization of the Voice Care as a diagnostic tool are planned to be carried out soon

A biologically inspired neural network controller for ballistic arm movements

<p>Abstract</p> <p>Background</p> <p>In humans, the implementation of multijoint tasks of the arm implies a highly complex integration of sensory information, sensorimotor transformations and motor planning. Computational models can be profitably used to better understand the mechanisms sub-serving motor control, thus providing useful perspectives and investigating different control hypotheses. To this purpose, the use of Artificial Neural Networks has been proposed to represent and interpret the movement of upper limb. In this paper, a neural network approach to the modelling of the motor control of a human arm during planar ballistic movements is presented.</p> <p>Methods</p> <p>The developed system is composed of three main computational blocks: 1) a parallel distributed learning scheme that aims at simulating the internal inverse model in the trajectory formation process; 2) a pulse generator, which is responsible for the creation of muscular synergies; and 3) a limb model based on two joints (two degrees of freedom) and six muscle-like actuators, that can accommodate for the biomechanical parameters of the arm. The learning paradigm of the neural controller is based on a pure exploration of the working space with no feedback signal. Kinematics provided by the system have been compared with those obtained in literature from experimental data of humans.</p> <p>Results</p> <p>The model reproduces kinematics of arm movements, with bell-shaped wrist velocity profiles and approximately straight trajectories, and gives rise to the generation of synergies for the execution of movements. The model allows achieving amplitude and direction errors of respectively 0.52 cm and 0.2 radians.</p> <p>Curvature values are similar to those encountered in experimental measures with humans.</p> <p>The neural controller also manages environmental modifications such as the insertion of different force fields acting on the end-effector.</p> <p>Conclusion</p> <p>The proposed system has been shown to properly simulate the development of internal models and to control the generation and execution of ballistic planar arm movements. Since the neural controller learns to manage movements on the basis of kinematic information and arm characteristics, it could in perspective command a neuroprosthesis instead of a biomechanical model of a human upper limb, and it could thus give rise to novel rehabilitation techniques.</p

Cardiac overexpression of melusin protects from dilated cardiomyopathy due to long-standing pressure overload.

We have previously shown that genetic ablation of melusin, a muscle specific beta 1 integrin interacting protein, accelerates left ventricle (LV) dilation and heart failure in response to pressure overload. Here we show that melusin expression was increased during compensated cardiac hypertrophy in mice subjected to 1 week pressure overload, but returned to basal levels in LV that have undergone dilation after 12 weeks of pressure overload. To better understand the role of melusin in cardiac remodeling, we overexpressed melusin in heart of transgenic mice. Echocardiography analysis indicated that melusin over-expression induced a mild cardiac hypertrophy in basal conditions (30% increase in interventricular septum thickness) with no obvious structural and functional alterations. After prolonged pressure overload (12 weeks), melusin overexpressing hearts underwent further hypertrophy retaining concentric LV remodeling and full contractile function, whereas wild-type LV showed pronounced chamber dilation with an impaired contractility. Analysis of signaling pathways indicated that melusin overexpression induced increased basal phosphorylation of GSK3beta and ERK1/2. Moreover, AKT, GSK3beta and ERK1/2 were hyper-phosphorylated on pressure overload in melusin overexpressing compared with wild-type mice. In addition, after 12 weeks of pressure overload LV of melusin overexpressing mice showed a very low level of cardiomyocyte apoptosis and stromal tissue deposition, as well as increased capillary density compared with wild-type. These results demonstrate that melusin overexpression allows prolonged concentric compensatory hypertrophy and protects against the transition toward cardiac dilation and failure in response to long-standing pressure overload

Union inclusiveness and temporary agency workers: the role of power resources and union ideology

This article investigates the determinants of union inclusiveness towards agency workers in Western Europe, using an index which combines unionization rates with dimensions of collective agreements covering agency workers. Using fuzzy-set Qualitative Comparative Analysis, we identify two combinations of conditions leading to inclusiveness: the ‘Northern path’ includes high union density, high bargaining coverage and high union authority, and is consistent with the power resources approach. The ‘Southern path’ combines high union authority, high bargaining coverage, statutory regulations of agency work and working-class orientation, showing that ideology rather than institutional incentives shapes union strategies towards the marginal workforce